Development, evaluation and application of a mixed-family selective breeding method for the Pacific oyster (Crassostrea gigas)
This dissertation develops mixed family selection for Pacific oysters using marker-based pedigree reconstruction. It focuses on improving the efficiency of parentage assignment, determining the optimum life stage to mix oyster families for rearing and selection, comparing mixed-family and separate-f...
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Format: | Doctoral or Postdoctoral Thesis |
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Oregon State University
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ftoregonstate:ir.library.oregonstate.edu:00000413w 2024-09-15T18:03:18+00:00 Development, evaluation and application of a mixed-family selective breeding method for the Pacific oyster (Crassostrea gigas) Matson, Sean Erik Meyer, Howard H. Langdon, Chris Banks, Michael Hohenboken, William Boudry, Pierre Animal Sciences Oregon State University. Graduate School https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/00000413w English [eng] eng unknown Oregon State University https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/00000413w All rights reserved Pacific oyster -- Breeding Pacific oyster -- Breeding -- Selection indexes Dissertation ftoregonstate 2024-07-22T18:06:04Z This dissertation develops mixed family selection for Pacific oysters using marker-based pedigree reconstruction. It focuses on improving the efficiency of parentage assignment, determining the optimum life stage to mix oyster families for rearing and selection, comparing mixed-family and separate-family selective breeding in the field, and applying the mixed method to estimate the heritability of shell shape. We developed novel computer software, P-LOCI (available at http://marineresearch.oregonstate.edu/genetics/PLOCI.html), which identifies the most efficient set of codominant markers for assigning parentage, accounting for marker linkage, mating design, null alleles and genotyping error, and found that the most efficient group of loci for assignment is not necessarily comprised of the top individually ranked loci, or best for all populations. We determined the optimum time to mix oyster families for rearing and selection in the field; overall, planting size is the most prudent time to mix families for MFS, due to high variability in family representation produced during larval and nursery stages. Mixing families at stages earlier than this for selection on field traits would require pre-planting genotyping of large samples for estimation of initial family representation, which would add substantial cost, or other special considerations. Rearing mixed family groups of oysters in the field yielded very similar results to rearing the same families separately, (r = 0.817 for two-site average individual weight at harvest), demonstrating it is unlikely associative effects are of great importance in the Pacific oyster. Our results show that the mixed method was well-suited for individual traits and walk-back selection, but would incur higher costs than the separate method to estimate survival with lower precision. Finally, we utilized the mixed method to estimate the heritability of shell shape using midparent-offspring regression; we estimated shell depth heritability as 0.404 ± 0.14 and shell width as 0.287 ± ... Doctoral or Postdoctoral Thesis Crassostrea gigas Pacific oyster ScholarsArchive@OSU (Oregon State University) |
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English unknown |
topic |
Pacific oyster -- Breeding Pacific oyster -- Breeding -- Selection indexes |
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Pacific oyster -- Breeding Pacific oyster -- Breeding -- Selection indexes Matson, Sean Erik Development, evaluation and application of a mixed-family selective breeding method for the Pacific oyster (Crassostrea gigas) |
topic_facet |
Pacific oyster -- Breeding Pacific oyster -- Breeding -- Selection indexes |
description |
This dissertation develops mixed family selection for Pacific oysters using marker-based pedigree reconstruction. It focuses on improving the efficiency of parentage assignment, determining the optimum life stage to mix oyster families for rearing and selection, comparing mixed-family and separate-family selective breeding in the field, and applying the mixed method to estimate the heritability of shell shape. We developed novel computer software, P-LOCI (available at http://marineresearch.oregonstate.edu/genetics/PLOCI.html), which identifies the most efficient set of codominant markers for assigning parentage, accounting for marker linkage, mating design, null alleles and genotyping error, and found that the most efficient group of loci for assignment is not necessarily comprised of the top individually ranked loci, or best for all populations. We determined the optimum time to mix oyster families for rearing and selection in the field; overall, planting size is the most prudent time to mix families for MFS, due to high variability in family representation produced during larval and nursery stages. Mixing families at stages earlier than this for selection on field traits would require pre-planting genotyping of large samples for estimation of initial family representation, which would add substantial cost, or other special considerations. Rearing mixed family groups of oysters in the field yielded very similar results to rearing the same families separately, (r = 0.817 for two-site average individual weight at harvest), demonstrating it is unlikely associative effects are of great importance in the Pacific oyster. Our results show that the mixed method was well-suited for individual traits and walk-back selection, but would incur higher costs than the separate method to estimate survival with lower precision. Finally, we utilized the mixed method to estimate the heritability of shell shape using midparent-offspring regression; we estimated shell depth heritability as 0.404 ± 0.14 and shell width as 0.287 ± ... |
author2 |
Meyer, Howard H. Langdon, Chris Banks, Michael Hohenboken, William Boudry, Pierre Animal Sciences Oregon State University. Graduate School |
format |
Doctoral or Postdoctoral Thesis |
author |
Matson, Sean Erik |
author_facet |
Matson, Sean Erik |
author_sort |
Matson, Sean Erik |
title |
Development, evaluation and application of a mixed-family selective breeding method for the Pacific oyster (Crassostrea gigas) |
title_short |
Development, evaluation and application of a mixed-family selective breeding method for the Pacific oyster (Crassostrea gigas) |
title_full |
Development, evaluation and application of a mixed-family selective breeding method for the Pacific oyster (Crassostrea gigas) |
title_fullStr |
Development, evaluation and application of a mixed-family selective breeding method for the Pacific oyster (Crassostrea gigas) |
title_full_unstemmed |
Development, evaluation and application of a mixed-family selective breeding method for the Pacific oyster (Crassostrea gigas) |
title_sort |
development, evaluation and application of a mixed-family selective breeding method for the pacific oyster (crassostrea gigas) |
publisher |
Oregon State University |
url |
https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/00000413w |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/00000413w |
op_rights |
All rights reserved |
_version_ |
1810440819839074304 |