STUDY OF SELENITE AND SELENOMETHIONINE EFFECT ON METHYLMERCURY IN VITRO TOXICITY
Methylmercury (MeHg) and selenium (Se) can be found at elevated concentrations in blood of marine mammals and both display modulatory effects on the immune system. Whereas mercury (Hg)-Se antagonism in liver of marine mammals is well known, the protective role of Se against Hg immunotoxicity in mari...
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ftorbi:oai:orbi.ulg.ac.be:2268/89158 2024-04-21T08:04:09+00:00 STUDY OF SELENITE AND SELENOMETHIONINE EFFECT ON METHYLMERCURY IN VITRO TOXICITY Dupont, Aurélie Siebert, Ursula Rosenberger, Tanja De Pauw-Gillet, Marie-Claire Das, Krishna Laboratoire d'Océanologie 2011-05-16 https://orbi.uliege.be/handle/2268/89158 en eng https://orbi.uliege.be/handle/2268/89158 info:hdl:2268/89158 Pollutant Responses in Marine Organisms, Long Beach, United States [US], du 15 mai 2011 au 18 mai 2011 methylmercury selenium harbor seal Life sciences Zoology Sciences du vivant Zoologie conference paper not in proceedings http://purl.org/coar/resource_type/c_18cp info:eu-repo/semantics/conferencePaper 2011 ftorbi 2024-03-27T14:43:45Z Methylmercury (MeHg) and selenium (Se) can be found at elevated concentrations in blood of marine mammals and both display modulatory effects on the immune system. Whereas mercury (Hg)-Se antagonism in liver of marine mammals is well known, the protective role of Se against Hg immunotoxicity in marine mammals has been poorly described. We propose here an in vitro approach using combined Hg and Se in vitro exposure of peripheral blood mononuclear cells (PBMCs) of the harbor seal (Phoca vitulina). PBMCs were isolated from the blood of 10 harbor seals and exposed to environmental concentrations of MeHg (1µM) and selenite or selenomethionine (5µM), respectively inorganic and organic forms of Se. MeHg leaded to a decrease of lymphocyte proliferation, to an increase of cells with compromised mitochondrial membrane potentials and cell death. Preliminary results evidenced that none of the two Se forms had a protective effect against MeHg toxicity, although cells were slightly stimulated by Se alone. Therefore MeHg expresses its toxicity among blood circulating lymphocytes in presence or absence of selenite or selenomethionine. Effects of methylmercury and selenium on harbor seal (Phoca vitulina) lymphocytes by functionnal, morphologic and proteomic analysis Conference Object harbor seal Phoca vitulina University of Liège: ORBi (Open Repository and Bibliography) |
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Open Polar |
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University of Liège: ORBi (Open Repository and Bibliography) |
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ftorbi |
language |
English |
topic |
methylmercury selenium harbor seal Life sciences Zoology Sciences du vivant Zoologie |
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methylmercury selenium harbor seal Life sciences Zoology Sciences du vivant Zoologie Dupont, Aurélie Siebert, Ursula Rosenberger, Tanja De Pauw-Gillet, Marie-Claire Das, Krishna STUDY OF SELENITE AND SELENOMETHIONINE EFFECT ON METHYLMERCURY IN VITRO TOXICITY |
topic_facet |
methylmercury selenium harbor seal Life sciences Zoology Sciences du vivant Zoologie |
description |
Methylmercury (MeHg) and selenium (Se) can be found at elevated concentrations in blood of marine mammals and both display modulatory effects on the immune system. Whereas mercury (Hg)-Se antagonism in liver of marine mammals is well known, the protective role of Se against Hg immunotoxicity in marine mammals has been poorly described. We propose here an in vitro approach using combined Hg and Se in vitro exposure of peripheral blood mononuclear cells (PBMCs) of the harbor seal (Phoca vitulina). PBMCs were isolated from the blood of 10 harbor seals and exposed to environmental concentrations of MeHg (1µM) and selenite or selenomethionine (5µM), respectively inorganic and organic forms of Se. MeHg leaded to a decrease of lymphocyte proliferation, to an increase of cells with compromised mitochondrial membrane potentials and cell death. Preliminary results evidenced that none of the two Se forms had a protective effect against MeHg toxicity, although cells were slightly stimulated by Se alone. Therefore MeHg expresses its toxicity among blood circulating lymphocytes in presence or absence of selenite or selenomethionine. Effects of methylmercury and selenium on harbor seal (Phoca vitulina) lymphocytes by functionnal, morphologic and proteomic analysis |
author2 |
Laboratoire d'Océanologie |
format |
Conference Object |
author |
Dupont, Aurélie Siebert, Ursula Rosenberger, Tanja De Pauw-Gillet, Marie-Claire Das, Krishna |
author_facet |
Dupont, Aurélie Siebert, Ursula Rosenberger, Tanja De Pauw-Gillet, Marie-Claire Das, Krishna |
author_sort |
Dupont, Aurélie |
title |
STUDY OF SELENITE AND SELENOMETHIONINE EFFECT ON METHYLMERCURY IN VITRO TOXICITY |
title_short |
STUDY OF SELENITE AND SELENOMETHIONINE EFFECT ON METHYLMERCURY IN VITRO TOXICITY |
title_full |
STUDY OF SELENITE AND SELENOMETHIONINE EFFECT ON METHYLMERCURY IN VITRO TOXICITY |
title_fullStr |
STUDY OF SELENITE AND SELENOMETHIONINE EFFECT ON METHYLMERCURY IN VITRO TOXICITY |
title_full_unstemmed |
STUDY OF SELENITE AND SELENOMETHIONINE EFFECT ON METHYLMERCURY IN VITRO TOXICITY |
title_sort |
study of selenite and selenomethionine effect on methylmercury in vitro toxicity |
publishDate |
2011 |
url |
https://orbi.uliege.be/handle/2268/89158 |
genre |
harbor seal Phoca vitulina |
genre_facet |
harbor seal Phoca vitulina |
op_source |
Pollutant Responses in Marine Organisms, Long Beach, United States [US], du 15 mai 2011 au 18 mai 2011 |
op_relation |
https://orbi.uliege.be/handle/2268/89158 info:hdl:2268/89158 |
_version_ |
1796943808077758464 |