Decalcifying odontocete ears following a routine protocol with RDO (R)
peer reviewed The study of the organ of Corti is essential to assess the impact of underwater noise on cetaceans. While classical histology techniques (including EDTA decalcification) have been previously considered, the process is time consuming. Independently from the histological technique, one o...
Published in: | Journal of Experimental Marine Biology and Ecology |
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Language: | English |
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2009
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Online Access: | https://orbi.uliege.be/handle/2268/76332 https://orbi.uliege.be/bitstream/2268/76332/1/Morell2009.pdf https://doi.org/10.1016/j.jembe.2009.05.005 |
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ftorbi:oai:orbi.ulg.ac.be:2268/76332 2024-04-21T08:07:43+00:00 Decalcifying odontocete ears following a routine protocol with RDO (R) Morell, M. Degollada, E. Alonso, J. M. Jauniaux, Thierry Andre, Marie-Elizabeth 2009 https://orbi.uliege.be/handle/2268/76332 https://orbi.uliege.be/bitstream/2268/76332/1/Morell2009.pdf https://doi.org/10.1016/j.jembe.2009.05.005 en eng Elsevier Science urn:issn:0022-0981 https://orbi.uliege.be/handle/2268/76332 info:hdl:2268/76332 https://orbi.uliege.be/bitstream/2268/76332/1/Morell2009.pdf doi:10.1016/j.jembe.2009.05.005 scopus-id:2-s2.0-67650700206 open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess Journal of Experimental Marine Biology and Ecology, 376 (2), 55-58 (2009) Life sciences Aquatic sciences & oceanology Sciences du vivant Sciences aquatiques & océanologie journal article http://purl.org/coar/resource_type/c_6501 info:eu-repo/semantics/article peer reviewed 2009 ftorbi https://doi.org/10.1016/j.jembe.2009.05.005 2024-03-27T14:56:04Z peer reviewed The study of the organ of Corti is essential to assess the impact of underwater noise on cetaceans. While classical histology techniques (including EDTA decalcification) have been previously considered, the process is time consuming. Independently from the histological technique, one of the challenging steps after extraction and fixation of the samples is to decalcify the bone envelope to access the cochlea without damaging the soft tissues. Here, we propose to use a fast commercial decalcifier (RDO (R)). 93 ears from 11 different odontocetes species stranded in the Mediterranean, Spanish North Atlantic and North Sea were used to precisely determine the decalcification time. Depending on the tympanic-periotic volume of the species, the decalcification time ranged from several hours to a few days, allowing a subsequently faster observation of the cochlear structures through routine microscope techniques. (C) 2009 Elsevier B.V. All rights reserved. Article in Journal/Newspaper North Atlantic University of Liège: ORBi (Open Repository and Bibliography) Journal of Experimental Marine Biology and Ecology 376 2 55 58 |
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Open Polar |
collection |
University of Liège: ORBi (Open Repository and Bibliography) |
op_collection_id |
ftorbi |
language |
English |
topic |
Life sciences Aquatic sciences & oceanology Sciences du vivant Sciences aquatiques & océanologie |
spellingShingle |
Life sciences Aquatic sciences & oceanology Sciences du vivant Sciences aquatiques & océanologie Morell, M. Degollada, E. Alonso, J. M. Jauniaux, Thierry Andre, Marie-Elizabeth Decalcifying odontocete ears following a routine protocol with RDO (R) |
topic_facet |
Life sciences Aquatic sciences & oceanology Sciences du vivant Sciences aquatiques & océanologie |
description |
peer reviewed The study of the organ of Corti is essential to assess the impact of underwater noise on cetaceans. While classical histology techniques (including EDTA decalcification) have been previously considered, the process is time consuming. Independently from the histological technique, one of the challenging steps after extraction and fixation of the samples is to decalcify the bone envelope to access the cochlea without damaging the soft tissues. Here, we propose to use a fast commercial decalcifier (RDO (R)). 93 ears from 11 different odontocetes species stranded in the Mediterranean, Spanish North Atlantic and North Sea were used to precisely determine the decalcification time. Depending on the tympanic-periotic volume of the species, the decalcification time ranged from several hours to a few days, allowing a subsequently faster observation of the cochlear structures through routine microscope techniques. (C) 2009 Elsevier B.V. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Morell, M. Degollada, E. Alonso, J. M. Jauniaux, Thierry Andre, Marie-Elizabeth |
author_facet |
Morell, M. Degollada, E. Alonso, J. M. Jauniaux, Thierry Andre, Marie-Elizabeth |
author_sort |
Morell, M. |
title |
Decalcifying odontocete ears following a routine protocol with RDO (R) |
title_short |
Decalcifying odontocete ears following a routine protocol with RDO (R) |
title_full |
Decalcifying odontocete ears following a routine protocol with RDO (R) |
title_fullStr |
Decalcifying odontocete ears following a routine protocol with RDO (R) |
title_full_unstemmed |
Decalcifying odontocete ears following a routine protocol with RDO (R) |
title_sort |
decalcifying odontocete ears following a routine protocol with rdo (r) |
publisher |
Elsevier Science |
publishDate |
2009 |
url |
https://orbi.uliege.be/handle/2268/76332 https://orbi.uliege.be/bitstream/2268/76332/1/Morell2009.pdf https://doi.org/10.1016/j.jembe.2009.05.005 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Journal of Experimental Marine Biology and Ecology, 376 (2), 55-58 (2009) |
op_relation |
urn:issn:0022-0981 https://orbi.uliege.be/handle/2268/76332 info:hdl:2268/76332 https://orbi.uliege.be/bitstream/2268/76332/1/Morell2009.pdf doi:10.1016/j.jembe.2009.05.005 scopus-id:2-s2.0-67650700206 |
op_rights |
open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.jembe.2009.05.005 |
container_title |
Journal of Experimental Marine Biology and Ecology |
container_volume |
376 |
container_issue |
2 |
container_start_page |
55 |
op_container_end_page |
58 |
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1796947735323082752 |