Methylmercury and selenium in vitro effects on harbor seal (Phoca vitulina) lymphocytes : a multidisciplinary approach
Methylmercury (MeHg) bioaccumulates along the food web, leading to the highest levels in tissues of predatory species. It constitutes the predominant form present in the blood of marine mammals. The blood cells, including the immune cells, are therefore exposed to the toxic properties of that chemic...
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ftorbi:oai:orbi.ulg.ac.be:2268/66134 2024-04-21T08:04:10+00:00 Methylmercury and selenium in vitro effects on harbor seal (Phoca vitulina) lymphocytes : a multidisciplinary approach Dupont, Aurélie Bouquegneau, Jean-Marie Das, Krishna De Pauw-Gillet, Marie-Claire Siebert, Ursula Rosenberger, Tanja Laboratoire d'Océanologie, Université de Liège, Liège, Belgique 2010-05-26 A0 https://orbi.uliege.be/handle/2268/66134 https://orbi.uliege.be/bitstream/2268/66134/1/Poster%20SETAC%20-%20Mai%202010%20%5bMode%20de%20compatibilit%c3%a9%5d.pdf en eng https://orbi.uliege.be/handle/2268/66134 info:hdl:2268/66134 https://orbi.uliege.be/bitstream/2268/66134/1/Poster%20SETAC%20-%20Mai%202010%20%5bMode%20de%20compatibilit%c3%a9%5d.pdf open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess 20th SETAC Europe Annual Meeting, Séville, Spain [ES], du 23 au 27 mai 2010 methylmercury selenium in vitro lymphocyte harbor seal phoca vitulina Life sciences Zoology Sciences du vivant Zoologie conference poster not in proceedings http://purl.org/coar/resource_type/c_18co info:eu-repo/semantics/conferencePoster 2010 ftorbi 2024-03-27T14:43:11Z Methylmercury (MeHg) bioaccumulates along the food web, leading to the highest levels in tissues of predatory species. It constitutes the predominant form present in the blood of marine mammals. The blood cells, including the immune cells, are therefore exposed to the toxic properties of that chemical. Nevertheless, selenium (Se) is an essential element absorbed concomitantly to MeHg which seems to modulate this toxicity. The goal of this study is to evaluate the immunotoxicity of MeHg on the harbor seal (Phoca vitulina) T lymphocytes, highly important in the adaptive immune response, and to investigate the modulating effect of Se on that toxicity. In parallel, the concentrations of MeHg, total mercury (T-Hg) and Se are determined in free-ranging harbour seal blood in order to follow their contamination levels. The T lymphocytes were isolated from the whole blood, exposed to various MeHg and Se concentrations and the exposure effects were estimated by functional tests including the evaluation of viability, proliferation, metabolic activity, DNA and protein synthesis, and by morphological analysis by transmission electron microscopy. The mean T-Hg concentration was 172 ± 143 µg/l of whole blood. The T lymphocytes cultures in vitro displayed a decreasing number of viable cells with increasing concentrations of MeHg, and numerous ultrastructural defects. The cells exposed to MeHg notably displayed distortion of the plasmic membrane, nucleus fragmentations, chromatin compaction, swelling mitochondrias and cytoplasmic vacuolisations. Those results highlighted various immunotoxic effects of MeHg, both at the functional and ultrastructural levels. The antagonistic role of Se on MeHg immunotoxicity is discussed. Conference Object harbor seal harbour seal Phoca vitulina University of Liège: ORBi (Open Repository and Bibliography) |
institution |
Open Polar |
collection |
University of Liège: ORBi (Open Repository and Bibliography) |
op_collection_id |
ftorbi |
language |
English |
topic |
methylmercury selenium in vitro lymphocyte harbor seal phoca vitulina Life sciences Zoology Sciences du vivant Zoologie |
spellingShingle |
methylmercury selenium in vitro lymphocyte harbor seal phoca vitulina Life sciences Zoology Sciences du vivant Zoologie Dupont, Aurélie Bouquegneau, Jean-Marie Das, Krishna Methylmercury and selenium in vitro effects on harbor seal (Phoca vitulina) lymphocytes : a multidisciplinary approach |
topic_facet |
methylmercury selenium in vitro lymphocyte harbor seal phoca vitulina Life sciences Zoology Sciences du vivant Zoologie |
description |
Methylmercury (MeHg) bioaccumulates along the food web, leading to the highest levels in tissues of predatory species. It constitutes the predominant form present in the blood of marine mammals. The blood cells, including the immune cells, are therefore exposed to the toxic properties of that chemical. Nevertheless, selenium (Se) is an essential element absorbed concomitantly to MeHg which seems to modulate this toxicity. The goal of this study is to evaluate the immunotoxicity of MeHg on the harbor seal (Phoca vitulina) T lymphocytes, highly important in the adaptive immune response, and to investigate the modulating effect of Se on that toxicity. In parallel, the concentrations of MeHg, total mercury (T-Hg) and Se are determined in free-ranging harbour seal blood in order to follow their contamination levels. The T lymphocytes were isolated from the whole blood, exposed to various MeHg and Se concentrations and the exposure effects were estimated by functional tests including the evaluation of viability, proliferation, metabolic activity, DNA and protein synthesis, and by morphological analysis by transmission electron microscopy. The mean T-Hg concentration was 172 ± 143 µg/l of whole blood. The T lymphocytes cultures in vitro displayed a decreasing number of viable cells with increasing concentrations of MeHg, and numerous ultrastructural defects. The cells exposed to MeHg notably displayed distortion of the plasmic membrane, nucleus fragmentations, chromatin compaction, swelling mitochondrias and cytoplasmic vacuolisations. Those results highlighted various immunotoxic effects of MeHg, both at the functional and ultrastructural levels. The antagonistic role of Se on MeHg immunotoxicity is discussed. |
author2 |
De Pauw-Gillet, Marie-Claire Siebert, Ursula Rosenberger, Tanja Laboratoire d'Océanologie, Université de Liège, Liège, Belgique |
format |
Conference Object |
author |
Dupont, Aurélie Bouquegneau, Jean-Marie Das, Krishna |
author_facet |
Dupont, Aurélie Bouquegneau, Jean-Marie Das, Krishna |
author_sort |
Dupont, Aurélie |
title |
Methylmercury and selenium in vitro effects on harbor seal (Phoca vitulina) lymphocytes : a multidisciplinary approach |
title_short |
Methylmercury and selenium in vitro effects on harbor seal (Phoca vitulina) lymphocytes : a multidisciplinary approach |
title_full |
Methylmercury and selenium in vitro effects on harbor seal (Phoca vitulina) lymphocytes : a multidisciplinary approach |
title_fullStr |
Methylmercury and selenium in vitro effects on harbor seal (Phoca vitulina) lymphocytes : a multidisciplinary approach |
title_full_unstemmed |
Methylmercury and selenium in vitro effects on harbor seal (Phoca vitulina) lymphocytes : a multidisciplinary approach |
title_sort |
methylmercury and selenium in vitro effects on harbor seal (phoca vitulina) lymphocytes : a multidisciplinary approach |
publishDate |
2010 |
url |
https://orbi.uliege.be/handle/2268/66134 https://orbi.uliege.be/bitstream/2268/66134/1/Poster%20SETAC%20-%20Mai%202010%20%5bMode%20de%20compatibilit%c3%a9%5d.pdf |
genre |
harbor seal harbour seal Phoca vitulina |
genre_facet |
harbor seal harbour seal Phoca vitulina |
op_source |
20th SETAC Europe Annual Meeting, Séville, Spain [ES], du 23 au 27 mai 2010 |
op_relation |
https://orbi.uliege.be/handle/2268/66134 info:hdl:2268/66134 https://orbi.uliege.be/bitstream/2268/66134/1/Poster%20SETAC%20-%20Mai%202010%20%5bMode%20de%20compatibilit%c3%a9%5d.pdf |
op_rights |
open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
_version_ |
1796943811206709248 |