Cortisol and epinephrine alter the adipose functions and the mobilization of PCBs in adipose tissue slices from elephant seal
peer reviewed Marine mammals are exposed to increasing intensities of anthropogenic stressors such as acoustic disturbance and contaminants. Correlative studies have suggested concerning shifts in behavioral and physiological status of stress-exposed individuals, which could alter the health and sur...
Published in: | Frontiers in Marine Science |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Frontiers Media SA
2023
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Online Access: | https://orbi.uliege.be/handle/2268/313681 https://orbi.uliege.be/bitstream/2268/313681/1/fmars-10-1290472.pdf https://doi.org/10.3389/fmars.2023.1290472 |
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ftorbi:oai:orbi.ulg.ac.be:2268/313681 2024-04-21T08:01:25+00:00 Cortisol and epinephrine alter the adipose functions and the mobilization of PCBs in adipose tissue slices from elephant seal Pirard, Laura Khudyakov, Jane I. Crocker, Daniel E. Van Hassel, Liesbeth Scholl, Georges Eppe, Gauthier Page, Melissa M. Rees, Jean-François Smith, Donald R. Debier, Cathy CART - Centre Interfacultaire d'Analyse des Résidus en Traces - ULiège BE 2023-11-17 https://orbi.uliege.be/handle/2268/313681 https://orbi.uliege.be/bitstream/2268/313681/1/fmars-10-1290472.pdf https://doi.org/10.3389/fmars.2023.1290472 en eng Frontiers Media SA https://www.frontiersin.org/articles/10.3389/fmars.2023.1290472/full urn:issn:2296-7745 https://orbi.uliege.be/handle/2268/313681 info:hdl:2268/313681 https://orbi.uliege.be/bitstream/2268/313681/1/fmars-10-1290472.pdf doi:10.3389/fmars.2023.1290472 scopus-id:2-s2.0-85178947227 open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess Frontiers in Marine Science, 10 (2023-11-17) adipose tissue cortisol elephant seal epinephrine leptin lipolysis PCB mobilization precision-cut slices Oceanography Global and Planetary Change Aquatic Science Water Science and Technology Environmental Science (miscellaneous) Ocean Engineering Physical chemical mathematical & earth Sciences Chemistry Life sciences Aquatic sciences & oceanology Physique chimie mathématiques & sciences de la terre Sciences du vivant Sciences aquatiques & océanologie journal article http://purl.org/coar/resource_type/c_6501 info:eu-repo/semantics/article peer reviewed 2023 ftorbi https://doi.org/10.3389/fmars.2023.1290472 2024-03-27T15:00:04Z peer reviewed Marine mammals are exposed to increasing intensities of anthropogenic stressors such as acoustic disturbance and contaminants. Correlative studies have suggested concerning shifts in behavioral and physiological status of stress-exposed individuals, which could alter the health and survival of marine mammal populations. However, functional studies of the effects of multiple stressors on marine mammals are lacking. To fill in this gap, we recently developed an ex vivo approach of precision-cut adipose tissue slices (PCATS) to study the impact of stressors on the function of an essential endocrine organ: the adipose tissue. In the present study, we investigated the impact of hormones associated with the stress response on adipose tissue from northern elephant seals (Mirounga angustirostris). Blubber biopsies were collected from 17 weaned northern elephant seal pups, separated into inner and outer layers, dissected into PCATS, and cultured for 48 hours. To mimic prolonged and short-term exposure to physiological stress, PCATS were treated with 2 µM cortisol (CORT) for the entire 48 hours or 100 nM epinephrine (EPI) for the last 12 hours of culture, respectively. Hormones were applied individually or in combination. CORT and EPI exhibited an interacting, blubber layer-dependent, effect on adipose tissue biology, as quantified by gene expression in PCATS, and release of glycerol, free fatty acids, leptin and polychlorinated biphenyls into culture media. EPI stimulated a higher rate of lipolysis than CORT in PCATS from both blubber layers. The combination of CORT and EPI upregulated the expression of adipose triglyceride lipase in inner blubber PCATS and downregulated hormone-sensitive lipase in outer blubber-derived PCATS. Expression of the leptin gene and secretion of the leptin adipokine were both decreased by EPI, while addition of CORT attenuated this effect in inner blubber PCATS only. CORT also increased the expression of the antioxidant enzyme glutathione peroxidase 3 in PCATS from both blubber ... Article in Journal/Newspaper Elephant Seal Elephant Seals University of Liège: ORBi (Open Repository and Bibliography) Frontiers in Marine Science 10 |
institution |
Open Polar |
collection |
University of Liège: ORBi (Open Repository and Bibliography) |
op_collection_id |
ftorbi |
language |
English |
topic |
adipose tissue cortisol elephant seal epinephrine leptin lipolysis PCB mobilization precision-cut slices Oceanography Global and Planetary Change Aquatic Science Water Science and Technology Environmental Science (miscellaneous) Ocean Engineering Physical chemical mathematical & earth Sciences Chemistry Life sciences Aquatic sciences & oceanology Physique chimie mathématiques & sciences de la terre Sciences du vivant Sciences aquatiques & océanologie |
spellingShingle |
adipose tissue cortisol elephant seal epinephrine leptin lipolysis PCB mobilization precision-cut slices Oceanography Global and Planetary Change Aquatic Science Water Science and Technology Environmental Science (miscellaneous) Ocean Engineering Physical chemical mathematical & earth Sciences Chemistry Life sciences Aquatic sciences & oceanology Physique chimie mathématiques & sciences de la terre Sciences du vivant Sciences aquatiques & océanologie Pirard, Laura Khudyakov, Jane I. Crocker, Daniel E. Van Hassel, Liesbeth Scholl, Georges Eppe, Gauthier Page, Melissa M. Rees, Jean-François Smith, Donald R. Debier, Cathy Cortisol and epinephrine alter the adipose functions and the mobilization of PCBs in adipose tissue slices from elephant seal |
topic_facet |
adipose tissue cortisol elephant seal epinephrine leptin lipolysis PCB mobilization precision-cut slices Oceanography Global and Planetary Change Aquatic Science Water Science and Technology Environmental Science (miscellaneous) Ocean Engineering Physical chemical mathematical & earth Sciences Chemistry Life sciences Aquatic sciences & oceanology Physique chimie mathématiques & sciences de la terre Sciences du vivant Sciences aquatiques & océanologie |
description |
peer reviewed Marine mammals are exposed to increasing intensities of anthropogenic stressors such as acoustic disturbance and contaminants. Correlative studies have suggested concerning shifts in behavioral and physiological status of stress-exposed individuals, which could alter the health and survival of marine mammal populations. However, functional studies of the effects of multiple stressors on marine mammals are lacking. To fill in this gap, we recently developed an ex vivo approach of precision-cut adipose tissue slices (PCATS) to study the impact of stressors on the function of an essential endocrine organ: the adipose tissue. In the present study, we investigated the impact of hormones associated with the stress response on adipose tissue from northern elephant seals (Mirounga angustirostris). Blubber biopsies were collected from 17 weaned northern elephant seal pups, separated into inner and outer layers, dissected into PCATS, and cultured for 48 hours. To mimic prolonged and short-term exposure to physiological stress, PCATS were treated with 2 µM cortisol (CORT) for the entire 48 hours or 100 nM epinephrine (EPI) for the last 12 hours of culture, respectively. Hormones were applied individually or in combination. CORT and EPI exhibited an interacting, blubber layer-dependent, effect on adipose tissue biology, as quantified by gene expression in PCATS, and release of glycerol, free fatty acids, leptin and polychlorinated biphenyls into culture media. EPI stimulated a higher rate of lipolysis than CORT in PCATS from both blubber layers. The combination of CORT and EPI upregulated the expression of adipose triglyceride lipase in inner blubber PCATS and downregulated hormone-sensitive lipase in outer blubber-derived PCATS. Expression of the leptin gene and secretion of the leptin adipokine were both decreased by EPI, while addition of CORT attenuated this effect in inner blubber PCATS only. CORT also increased the expression of the antioxidant enzyme glutathione peroxidase 3 in PCATS from both blubber ... |
author2 |
CART - Centre Interfacultaire d'Analyse des Résidus en Traces - ULiège BE |
format |
Article in Journal/Newspaper |
author |
Pirard, Laura Khudyakov, Jane I. Crocker, Daniel E. Van Hassel, Liesbeth Scholl, Georges Eppe, Gauthier Page, Melissa M. Rees, Jean-François Smith, Donald R. Debier, Cathy |
author_facet |
Pirard, Laura Khudyakov, Jane I. Crocker, Daniel E. Van Hassel, Liesbeth Scholl, Georges Eppe, Gauthier Page, Melissa M. Rees, Jean-François Smith, Donald R. Debier, Cathy |
author_sort |
Pirard, Laura |
title |
Cortisol and epinephrine alter the adipose functions and the mobilization of PCBs in adipose tissue slices from elephant seal |
title_short |
Cortisol and epinephrine alter the adipose functions and the mobilization of PCBs in adipose tissue slices from elephant seal |
title_full |
Cortisol and epinephrine alter the adipose functions and the mobilization of PCBs in adipose tissue slices from elephant seal |
title_fullStr |
Cortisol and epinephrine alter the adipose functions and the mobilization of PCBs in adipose tissue slices from elephant seal |
title_full_unstemmed |
Cortisol and epinephrine alter the adipose functions and the mobilization of PCBs in adipose tissue slices from elephant seal |
title_sort |
cortisol and epinephrine alter the adipose functions and the mobilization of pcbs in adipose tissue slices from elephant seal |
publisher |
Frontiers Media SA |
publishDate |
2023 |
url |
https://orbi.uliege.be/handle/2268/313681 https://orbi.uliege.be/bitstream/2268/313681/1/fmars-10-1290472.pdf https://doi.org/10.3389/fmars.2023.1290472 |
genre |
Elephant Seal Elephant Seals |
genre_facet |
Elephant Seal Elephant Seals |
op_source |
Frontiers in Marine Science, 10 (2023-11-17) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fmars.2023.1290472/full urn:issn:2296-7745 https://orbi.uliege.be/handle/2268/313681 info:hdl:2268/313681 https://orbi.uliege.be/bitstream/2268/313681/1/fmars-10-1290472.pdf doi:10.3389/fmars.2023.1290472 scopus-id:2-s2.0-85178947227 |
op_rights |
open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3389/fmars.2023.1290472 |
container_title |
Frontiers in Marine Science |
container_volume |
10 |
_version_ |
1796941601839251456 |