Free-living, psychrotrophic bacteria of the genus Psychrobacter are descendents of pathobionts
peer reviewed Host-adapted microorganisms are generally assumed to have evolved from free-living, environmental microorganisms, as examples of the reverse process are rare. In the phylum Gammaproteobacteria, family Moraxellaceae, the genus Psychrobacter includes strains from a broad ecological distr...
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American Society for Microbiology
2021
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Online Access: | https://orbi.uliege.be/handle/2268/261759 https://orbi.uliege.be/bitstream/2268/261759/1/welter%20michaux%20psychotropic%20bacterias.pdf https://doi.org/10.1128/mSystems.00258-21 |
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ftorbi:oai:orbi.ulg.ac.be:2268/261759 2024-04-21T08:10:37+00:00 Free-living, psychrotrophic bacteria of the genus Psychrobacter are descendents of pathobionts Welter, D Ruaud, A Henzeler, Z De jong, H Van Coeverden de Groot, Peter Michaux, Johan Gormezano, L Waters, J. Youngblut, L. Ley, R 2021 https://orbi.uliege.be/handle/2268/261759 https://orbi.uliege.be/bitstream/2268/261759/1/welter%20michaux%20psychotropic%20bacterias.pdf https://doi.org/10.1128/mSystems.00258-21 en eng American Society for Microbiology urn:issn:2379-5077 https://orbi.uliege.be/handle/2268/261759 info:hdl:2268/261759 https://orbi.uliege.be/bitstream/2268/261759/1/welter%20michaux%20psychotropic%20bacterias.pdf doi:10.1128/mSystems.00258-21 scopus-id:2-s2.0-85104731528 open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess mSystems (2021) Life sciences Zoology Sciences du vivant Zoologie journal article http://purl.org/coar/resource_type/c_6501 info:eu-repo/semantics/article peer reviewed 2021 ftorbi https://doi.org/10.1128/mSystems.00258-21 2024-03-27T14:55:22Z peer reviewed Host-adapted microorganisms are generally assumed to have evolved from free-living, environmental microorganisms, as examples of the reverse process are rare. In the phylum Gammaproteobacteria, family Moraxellaceae, the genus Psychrobacter includes strains from a broad ecological distribution including animal bodies as well as sea ice and other nonhost environments. To elucidate the relationship between these ecological niches and Psychrobacter’s evolutionary history, we performed tandem genomic analyses with phenotyping of 85 Psychrobacter accessions. Phylogenomic analysis of the family Moraxellaceae reveals that basal members of the Psychrobacter clade are Moraxella spp., a group of often-pathogenic organisms. Psychrobacter exhibited two broad growth patterns in our phenotypic screen: one group that we called the “flexible ecotype” (FE) had the ability to grow between 4 and 37°C, and the other, which we called the “restricted ecotype” (RE), could grow between 4 and 25°C. The FE group includes phylogenetically basal strains, and FE strains exhibit increased transposon copy numbers, smaller genomes, and a higher likelihood to be bile salt resistant. The RE group contains only phylogenetically derived strains and has increased proportions of lipid metabolism and biofilm formation genes, functions that are adaptive to cold stress. In a 16S rRNA gene survey of polar bear fecal samples, we detect both FE and RE strains, but in in vivo colonizations of gnotobiotic mice, only FE strains persist. Our results indicate the ability to grow at 37°C, seemingly necessary for mammalian gut colonization, is an ancestral trait for Psychrobacter, which likely evolved from a pathobiont. Article in Journal/Newspaper polar bear Sea ice University of Liège: ORBi (Open Repository and Bibliography) mSystems 6 2 |
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Open Polar |
collection |
University of Liège: ORBi (Open Repository and Bibliography) |
op_collection_id |
ftorbi |
language |
English |
topic |
Life sciences Zoology Sciences du vivant Zoologie |
spellingShingle |
Life sciences Zoology Sciences du vivant Zoologie Welter, D Ruaud, A Henzeler, Z De jong, H Van Coeverden de Groot, Peter Michaux, Johan Gormezano, L Waters, J. Youngblut, L. Ley, R Free-living, psychrotrophic bacteria of the genus Psychrobacter are descendents of pathobionts |
topic_facet |
Life sciences Zoology Sciences du vivant Zoologie |
description |
peer reviewed Host-adapted microorganisms are generally assumed to have evolved from free-living, environmental microorganisms, as examples of the reverse process are rare. In the phylum Gammaproteobacteria, family Moraxellaceae, the genus Psychrobacter includes strains from a broad ecological distribution including animal bodies as well as sea ice and other nonhost environments. To elucidate the relationship between these ecological niches and Psychrobacter’s evolutionary history, we performed tandem genomic analyses with phenotyping of 85 Psychrobacter accessions. Phylogenomic analysis of the family Moraxellaceae reveals that basal members of the Psychrobacter clade are Moraxella spp., a group of often-pathogenic organisms. Psychrobacter exhibited two broad growth patterns in our phenotypic screen: one group that we called the “flexible ecotype” (FE) had the ability to grow between 4 and 37°C, and the other, which we called the “restricted ecotype” (RE), could grow between 4 and 25°C. The FE group includes phylogenetically basal strains, and FE strains exhibit increased transposon copy numbers, smaller genomes, and a higher likelihood to be bile salt resistant. The RE group contains only phylogenetically derived strains and has increased proportions of lipid metabolism and biofilm formation genes, functions that are adaptive to cold stress. In a 16S rRNA gene survey of polar bear fecal samples, we detect both FE and RE strains, but in in vivo colonizations of gnotobiotic mice, only FE strains persist. Our results indicate the ability to grow at 37°C, seemingly necessary for mammalian gut colonization, is an ancestral trait for Psychrobacter, which likely evolved from a pathobiont. |
format |
Article in Journal/Newspaper |
author |
Welter, D Ruaud, A Henzeler, Z De jong, H Van Coeverden de Groot, Peter Michaux, Johan Gormezano, L Waters, J. Youngblut, L. Ley, R |
author_facet |
Welter, D Ruaud, A Henzeler, Z De jong, H Van Coeverden de Groot, Peter Michaux, Johan Gormezano, L Waters, J. Youngblut, L. Ley, R |
author_sort |
Welter, D |
title |
Free-living, psychrotrophic bacteria of the genus Psychrobacter are descendents of pathobionts |
title_short |
Free-living, psychrotrophic bacteria of the genus Psychrobacter are descendents of pathobionts |
title_full |
Free-living, psychrotrophic bacteria of the genus Psychrobacter are descendents of pathobionts |
title_fullStr |
Free-living, psychrotrophic bacteria of the genus Psychrobacter are descendents of pathobionts |
title_full_unstemmed |
Free-living, psychrotrophic bacteria of the genus Psychrobacter are descendents of pathobionts |
title_sort |
free-living, psychrotrophic bacteria of the genus psychrobacter are descendents of pathobionts |
publisher |
American Society for Microbiology |
publishDate |
2021 |
url |
https://orbi.uliege.be/handle/2268/261759 https://orbi.uliege.be/bitstream/2268/261759/1/welter%20michaux%20psychotropic%20bacterias.pdf https://doi.org/10.1128/mSystems.00258-21 |
genre |
polar bear Sea ice |
genre_facet |
polar bear Sea ice |
op_source |
mSystems (2021) |
op_relation |
urn:issn:2379-5077 https://orbi.uliege.be/handle/2268/261759 info:hdl:2268/261759 https://orbi.uliege.be/bitstream/2268/261759/1/welter%20michaux%20psychotropic%20bacterias.pdf doi:10.1128/mSystems.00258-21 scopus-id:2-s2.0-85104731528 |
op_rights |
open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1128/mSystems.00258-21 |
container_title |
mSystems |
container_volume |
6 |
container_issue |
2 |
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1796952138564239360 |