Cloning of the prepro C-RFa gene and brain localization of the active peptide in Salmo salar
peer reviewed In all vertebrates, the synthesis and release of prolactin (Prl) from pituitary lactotroph cells is tightly controlled by hypothalamic factors. We have cloned and characterized a hypothalamic cDNA from Atlantic salmon (Salmo salar) encoding C-RFa, a peptide structurally related to mamm...
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ftorbi:oai:orbi.ulg.ac.be:2268/23649 2024-10-13T14:06:08+00:00 Cloning of the prepro C-RFa gene and brain localization of the active peptide in Salmo salar Montefusco-Siegmund, R. A. Romero, A. Kausel, Gudrun Muller, Marc Fujimoto, M. Figueroa, J. AFFISH-RC - Applied and Fundamental FISH Research Center - ULiège Giga-Development and Stem Cells - ULiège 2006-08 https://orbi.uliege.be/handle/2268/23649 https://doi.org/10.1007/s00441-006-0168-6 en eng Springer urn:issn:0302-766X urn:issn:1432-0878 https://orbi.uliege.be/handle/2268/23649 info:hdl:2268/23649 info:pmid:16557384 restricted access http://purl.org/coar/access_right/c_16ec info:eu-repo/semantics/restrictedAccess Cell and Tissue Research, 325 (2), 277-285 (2006-08) hypothalamus pituitary rostral pars distalis C-RFa peptide immunohistochemistry reverse transcription/polymerase chain reaction Salmo salar (Teleostei) Life sciences Genetics & genetic processes Zoology Anatomy (cytology histology embryology.) & physiology Sciences du vivant Génétique & processus génétiques Zoologie Anatomie (cytologie histologie embryologie.) & physiologie journal article http://purl.org/coar/resource_type/c_6501 info:eu-repo/semantics/article peer reviewed 2006 ftorbi https://doi.org/10.1007/s00441-006-0168-6 2024-09-27T07:01:47Z peer reviewed In all vertebrates, the synthesis and release of prolactin (Prl) from pituitary lactotroph cells is tightly controlled by hypothalamic factors. We have cloned and characterized a hypothalamic cDNA from Atlantic salmon (Salmo salar) encoding C-RFa, a peptide structurally related to mammalian Prl-releasing peptide (PrRP). The deduced preprohormone precursor is composed of 155 amino acid residues presenting a 87.1% similarity to chum salmon C-RFa and a 100% similarity to all fish C-RFa in the bioactive precursor motifs. C-RFa-immunoreactive perikarya and fibres were located in the brain of S. salar, especially in the hypothalamus, olfactory tract, optic tectum and cerebellum. In contrast, immunolabelled fibres were not observed in the pituitary stalk or in the hypophysis. However, interestingly, we detected immunolabelled cells in the rostral pars distalis of the pituitary in the basolateral region in which Prl is synthesized. These results were confirmed by obtaining a strong signal by using reverse transcription/polymerase chain reaction (RTPCR) on mRNA from both hypothalamus and pituitary. These data show, for the first time, by immunohistochemistry and RT-PCR, that C-RFa is produced in pituitary cells. Finally, based on these results, a possible function for CRFa as a locally produced PrRP in this teleost is discussed. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Liège: ORBi (Open Repository and Bibliography) Cell and Tissue Research 325 2 277 285 |
institution |
Open Polar |
collection |
University of Liège: ORBi (Open Repository and Bibliography) |
op_collection_id |
ftorbi |
language |
English |
topic |
hypothalamus pituitary rostral pars distalis C-RFa peptide immunohistochemistry reverse transcription/polymerase chain reaction Salmo salar (Teleostei) Life sciences Genetics & genetic processes Zoology Anatomy (cytology histology embryology.) & physiology Sciences du vivant Génétique & processus génétiques Zoologie Anatomie (cytologie histologie embryologie.) & physiologie |
spellingShingle |
hypothalamus pituitary rostral pars distalis C-RFa peptide immunohistochemistry reverse transcription/polymerase chain reaction Salmo salar (Teleostei) Life sciences Genetics & genetic processes Zoology Anatomy (cytology histology embryology.) & physiology Sciences du vivant Génétique & processus génétiques Zoologie Anatomie (cytologie histologie embryologie.) & physiologie Montefusco-Siegmund, R. A. Romero, A. Kausel, Gudrun Muller, Marc Fujimoto, M. Figueroa, J. Cloning of the prepro C-RFa gene and brain localization of the active peptide in Salmo salar |
topic_facet |
hypothalamus pituitary rostral pars distalis C-RFa peptide immunohistochemistry reverse transcription/polymerase chain reaction Salmo salar (Teleostei) Life sciences Genetics & genetic processes Zoology Anatomy (cytology histology embryology.) & physiology Sciences du vivant Génétique & processus génétiques Zoologie Anatomie (cytologie histologie embryologie.) & physiologie |
description |
peer reviewed In all vertebrates, the synthesis and release of prolactin (Prl) from pituitary lactotroph cells is tightly controlled by hypothalamic factors. We have cloned and characterized a hypothalamic cDNA from Atlantic salmon (Salmo salar) encoding C-RFa, a peptide structurally related to mammalian Prl-releasing peptide (PrRP). The deduced preprohormone precursor is composed of 155 amino acid residues presenting a 87.1% similarity to chum salmon C-RFa and a 100% similarity to all fish C-RFa in the bioactive precursor motifs. C-RFa-immunoreactive perikarya and fibres were located in the brain of S. salar, especially in the hypothalamus, olfactory tract, optic tectum and cerebellum. In contrast, immunolabelled fibres were not observed in the pituitary stalk or in the hypophysis. However, interestingly, we detected immunolabelled cells in the rostral pars distalis of the pituitary in the basolateral region in which Prl is synthesized. These results were confirmed by obtaining a strong signal by using reverse transcription/polymerase chain reaction (RTPCR) on mRNA from both hypothalamus and pituitary. These data show, for the first time, by immunohistochemistry and RT-PCR, that C-RFa is produced in pituitary cells. Finally, based on these results, a possible function for CRFa as a locally produced PrRP in this teleost is discussed. |
author2 |
AFFISH-RC - Applied and Fundamental FISH Research Center - ULiège Giga-Development and Stem Cells - ULiège |
format |
Article in Journal/Newspaper |
author |
Montefusco-Siegmund, R. A. Romero, A. Kausel, Gudrun Muller, Marc Fujimoto, M. Figueroa, J. |
author_facet |
Montefusco-Siegmund, R. A. Romero, A. Kausel, Gudrun Muller, Marc Fujimoto, M. Figueroa, J. |
author_sort |
Montefusco-Siegmund, R. A. |
title |
Cloning of the prepro C-RFa gene and brain localization of the active peptide in Salmo salar |
title_short |
Cloning of the prepro C-RFa gene and brain localization of the active peptide in Salmo salar |
title_full |
Cloning of the prepro C-RFa gene and brain localization of the active peptide in Salmo salar |
title_fullStr |
Cloning of the prepro C-RFa gene and brain localization of the active peptide in Salmo salar |
title_full_unstemmed |
Cloning of the prepro C-RFa gene and brain localization of the active peptide in Salmo salar |
title_sort |
cloning of the prepro c-rfa gene and brain localization of the active peptide in salmo salar |
publisher |
Springer |
publishDate |
2006 |
url |
https://orbi.uliege.be/handle/2268/23649 https://doi.org/10.1007/s00441-006-0168-6 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Cell and Tissue Research, 325 (2), 277-285 (2006-08) |
op_relation |
urn:issn:0302-766X urn:issn:1432-0878 https://orbi.uliege.be/handle/2268/23649 info:hdl:2268/23649 info:pmid:16557384 |
op_rights |
restricted access http://purl.org/coar/access_right/c_16ec info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1007/s00441-006-0168-6 |
container_title |
Cell and Tissue Research |
container_volume |
325 |
container_issue |
2 |
container_start_page |
277 |
op_container_end_page |
285 |
_version_ |
1812812189927473152 |