Highest diversity of cyanobacterial on granite substrates in the Sør Rondane Mountains
In the Sør Rondane Mountains (Dronning Maud Land, East Antarctica), cyanobacteria are mainly present in sheltered spots in rocky areas, supporting the importance of micro-topographic and -climatic conditions. Biofilms, crusts and gravels on granite and gneiss substrates were sampled in 2009-2010 nea...
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ftorbi:oai:orbi.ulg.ac.be:2268/227175 2024-10-13T14:01:45+00:00 Highest diversity of cyanobacterial on granite substrates in the Sør Rondane Mountains Savaglia, Valentina Namsraev, Zorigto Mano, Marie-José Wilmotte, Annick CIP - Centre d'Ingénierie des Protéines - ULiège 2018 https://orbi.uliege.be/handle/2268/227175 https://orbi.uliege.be/bitstream/2268/227175/1/poster%20Davos2%20%283%29.pdf en eng https://orbi.uliege.be/handle/2268/227175 info:hdl:2268/227175 https://orbi.uliege.be/bitstream/2268/227175/1/poster%20Davos2%20%283%29.pdf open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess POLAR 2018 Conference - Where the Poles come together, from 19-06-2018 to 23-06-2018 Cyanobacteria Ice-free regions DGGE Antarctica Life sciences Microbiology Sciences du vivant Microbiologie conference poster not in proceedings http://purl.org/coar/resource_type/c_18co info:eu-repo/semantics/conferencePoster 2018 ftorbi 2024-09-27T07:01:34Z In the Sør Rondane Mountains (Dronning Maud Land, East Antarctica), cyanobacteria are mainly present in sheltered spots in rocky areas, supporting the importance of micro-topographic and -climatic conditions. Biofilms, crusts and gravels on granite and gneiss substrates were sampled in 2009-2010 near the Belgian Princess Elisabeth Station and their morphological (126 samples) and molecular diversity (26 samples) was assessed. A DNA extraction protocol was designed for the taxa with large polysaccharidic sheaths. Based on microscopy, crusts were the richest samples followed by gravels. The most diverse communities were found on the granites. Based on DGGE of 16S rRNA gene, 28 OTUs shared at least 97.5% of 16S rRNA similarity. OTUs’ richness varied between 1 and 5 per sample. A comparison of morphological and DGGE analyses showed that for most samples, the number of morphotypes was higher than the number of OTUs. However, both methods were congruent in defining the richest sites. The most frequently observed OTU was affiliated to Phormidium/Microcoleus sp. No OTU was common to all the 10 sites. The higher diversity on granite substrates (mainly big boulders) could be explained by higher stability of the underlaying rock and its ability to keep water on the surface, thus creating favorable conditions for development of organisms. Further insights will be given by the analysis of more samples with High-Throughput amplicon sequencing. Conference Object Antarc* Antarctica Dronning Maud Land East Antarctica University of Liège: ORBi (Open Repository and Bibliography) Dronning Maud Land East Antarctica Sør Rondane Mountains ENVELOPE(25.000,25.000,-72.000,-72.000) Sør-Rondane ENVELOPE(25.000,25.000,-72.000,-72.000) |
institution |
Open Polar |
collection |
University of Liège: ORBi (Open Repository and Bibliography) |
op_collection_id |
ftorbi |
language |
English |
topic |
Cyanobacteria Ice-free regions DGGE Antarctica Life sciences Microbiology Sciences du vivant Microbiologie |
spellingShingle |
Cyanobacteria Ice-free regions DGGE Antarctica Life sciences Microbiology Sciences du vivant Microbiologie Savaglia, Valentina Namsraev, Zorigto Mano, Marie-José Wilmotte, Annick Highest diversity of cyanobacterial on granite substrates in the Sør Rondane Mountains |
topic_facet |
Cyanobacteria Ice-free regions DGGE Antarctica Life sciences Microbiology Sciences du vivant Microbiologie |
description |
In the Sør Rondane Mountains (Dronning Maud Land, East Antarctica), cyanobacteria are mainly present in sheltered spots in rocky areas, supporting the importance of micro-topographic and -climatic conditions. Biofilms, crusts and gravels on granite and gneiss substrates were sampled in 2009-2010 near the Belgian Princess Elisabeth Station and their morphological (126 samples) and molecular diversity (26 samples) was assessed. A DNA extraction protocol was designed for the taxa with large polysaccharidic sheaths. Based on microscopy, crusts were the richest samples followed by gravels. The most diverse communities were found on the granites. Based on DGGE of 16S rRNA gene, 28 OTUs shared at least 97.5% of 16S rRNA similarity. OTUs’ richness varied between 1 and 5 per sample. A comparison of morphological and DGGE analyses showed that for most samples, the number of morphotypes was higher than the number of OTUs. However, both methods were congruent in defining the richest sites. The most frequently observed OTU was affiliated to Phormidium/Microcoleus sp. No OTU was common to all the 10 sites. The higher diversity on granite substrates (mainly big boulders) could be explained by higher stability of the underlaying rock and its ability to keep water on the surface, thus creating favorable conditions for development of organisms. Further insights will be given by the analysis of more samples with High-Throughput amplicon sequencing. |
author2 |
CIP - Centre d'Ingénierie des Protéines - ULiège |
format |
Conference Object |
author |
Savaglia, Valentina Namsraev, Zorigto Mano, Marie-José Wilmotte, Annick |
author_facet |
Savaglia, Valentina Namsraev, Zorigto Mano, Marie-José Wilmotte, Annick |
author_sort |
Savaglia, Valentina |
title |
Highest diversity of cyanobacterial on granite substrates in the Sør Rondane Mountains |
title_short |
Highest diversity of cyanobacterial on granite substrates in the Sør Rondane Mountains |
title_full |
Highest diversity of cyanobacterial on granite substrates in the Sør Rondane Mountains |
title_fullStr |
Highest diversity of cyanobacterial on granite substrates in the Sør Rondane Mountains |
title_full_unstemmed |
Highest diversity of cyanobacterial on granite substrates in the Sør Rondane Mountains |
title_sort |
highest diversity of cyanobacterial on granite substrates in the sør rondane mountains |
publishDate |
2018 |
url |
https://orbi.uliege.be/handle/2268/227175 https://orbi.uliege.be/bitstream/2268/227175/1/poster%20Davos2%20%283%29.pdf |
long_lat |
ENVELOPE(25.000,25.000,-72.000,-72.000) ENVELOPE(25.000,25.000,-72.000,-72.000) |
geographic |
Dronning Maud Land East Antarctica Sør Rondane Mountains Sør-Rondane |
geographic_facet |
Dronning Maud Land East Antarctica Sør Rondane Mountains Sør-Rondane |
genre |
Antarc* Antarctica Dronning Maud Land East Antarctica |
genre_facet |
Antarc* Antarctica Dronning Maud Land East Antarctica |
op_source |
POLAR 2018 Conference - Where the Poles come together, from 19-06-2018 to 23-06-2018 |
op_relation |
https://orbi.uliege.be/handle/2268/227175 info:hdl:2268/227175 https://orbi.uliege.be/bitstream/2268/227175/1/poster%20Davos2%20%283%29.pdf |
op_rights |
open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
_version_ |
1812812684703301632 |