Cyanobacteria inhabiting biological soil crusts of a polar desert: Sør Rondane Mountains, Antarctica
peer reviewed Molecular and morphological methods were applied to study cyanobacterial community composition in biological soil crusts (BSCs) from four areas (two nunataks and two ridges) in the Sør Rondane Mountains, Antarctica. The sampling sites serve as control areas for open top chambers (OTCs)...
Published in: | Systematic and Applied Microbiology |
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Online Access: | https://orbi.uliege.be/handle/2268/218320 https://doi.org/10.1016/j.syapm.2018.01.006 |
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ftorbi:oai:orbi.ulg.ac.be:2268/218320 2024-10-13T14:02:43+00:00 Cyanobacteria inhabiting biological soil crusts of a polar desert: Sør Rondane Mountains, Antarctica Les cyanobactéries habitant les croutes biotiques d'un désert polaire Pushkareva, Ekaterina Pessi, Igor S Namsaraev, Zorigto Mano, Marie-José Elster, Josef Wilmotte, Annick CIP - Centre d'Ingénierie des Protéines - ULiège 2018 https://orbi.uliege.be/handle/2268/218320 https://doi.org/10.1016/j.syapm.2018.01.006 en eng Elsevier https://www.sciencedirect.com/science/article/pii/S0723202018300274 urn:issn:0723-2020 urn:issn:1618-0984 https://orbi.uliege.be/handle/2268/218320 info:hdl:2268/218320 doi:10.1016/j.syapm.2018.01.006 info:pmid:29452715 restricted access http://purl.org/coar/access_right/c_16ec info:eu-repo/semantics/restrictedAccess Systematic and Applied Microbiology, 41, 363-373 (2018) cyanobacteria Biological Soil Crusts Antarctica Biodiversity Open Top Chambers Life sciences Microbiology Sciences du vivant Microbiologie journal article http://purl.org/coar/resource_type/c_6501 info:eu-repo/semantics/article peer reviewed 2018 ftorbi https://doi.org/10.1016/j.syapm.2018.01.006 2024-09-27T07:01:46Z peer reviewed Molecular and morphological methods were applied to study cyanobacterial community composition in biological soil crusts (BSCs) from four areas (two nunataks and two ridges) in the Sør Rondane Mountains, Antarctica. The sampling sites serve as control areas for open top chambers (OTCs) which were placed in 2010 at the time of sample collection and will be compared with BSC samples taken from the OTCs in future. Cyanobacterial cell biovolume was estimated using epifluorescence microscopy, which revealed the dominance of filamentous cyanobacteria in all studied sites except the Utsteinen ridge, where unicellular cyanobacteria were the most abundant. Cyanobacterial diversity was studied by a combination of molecular fingerprinting methods based on the 16S rRNA gene (denaturing gradient gel electrophoresis (DGGE) and 454 pyrosequencing) using cyanobacteria specific primers. The number of DGGE sequences obtained per site was variable and, therefore, a high-throughput method was later employed to improve the diversity coverage. Consistent with previous surveys in Antarctica, both methods showed that filamentous cyanobacteria such as Leptolyngbya sp., Phormidium sp. and Microcoleus sp. were dominant in the studied sites. In addition, the studied localities differed in substrate type, climatic conditions and soil parameters, which likely resulted in differences in cyanobacterial community composition. Furthermore, the BSC growing on gneiss pebbles had lower cyanobacterial abundances than BSCs associated to granitic substrates. BELDIVA Article in Journal/Newspaper Antarc* Antarctica polar desert University of Liège: ORBi (Open Repository and Bibliography) Sør Rondane Mountains ENVELOPE(25.000,25.000,-72.000,-72.000) Sør-Rondane ENVELOPE(25.000,25.000,-72.000,-72.000) Utsteinen ENVELOPE(23.342,23.342,-71.955,-71.955) Systematic and Applied Microbiology 41 4 363 373 |
institution |
Open Polar |
collection |
University of Liège: ORBi (Open Repository and Bibliography) |
op_collection_id |
ftorbi |
language |
English |
topic |
cyanobacteria Biological Soil Crusts Antarctica Biodiversity Open Top Chambers Life sciences Microbiology Sciences du vivant Microbiologie |
spellingShingle |
cyanobacteria Biological Soil Crusts Antarctica Biodiversity Open Top Chambers Life sciences Microbiology Sciences du vivant Microbiologie Pushkareva, Ekaterina Pessi, Igor S Namsaraev, Zorigto Mano, Marie-José Elster, Josef Wilmotte, Annick Cyanobacteria inhabiting biological soil crusts of a polar desert: Sør Rondane Mountains, Antarctica |
topic_facet |
cyanobacteria Biological Soil Crusts Antarctica Biodiversity Open Top Chambers Life sciences Microbiology Sciences du vivant Microbiologie |
description |
peer reviewed Molecular and morphological methods were applied to study cyanobacterial community composition in biological soil crusts (BSCs) from four areas (two nunataks and two ridges) in the Sør Rondane Mountains, Antarctica. The sampling sites serve as control areas for open top chambers (OTCs) which were placed in 2010 at the time of sample collection and will be compared with BSC samples taken from the OTCs in future. Cyanobacterial cell biovolume was estimated using epifluorescence microscopy, which revealed the dominance of filamentous cyanobacteria in all studied sites except the Utsteinen ridge, where unicellular cyanobacteria were the most abundant. Cyanobacterial diversity was studied by a combination of molecular fingerprinting methods based on the 16S rRNA gene (denaturing gradient gel electrophoresis (DGGE) and 454 pyrosequencing) using cyanobacteria specific primers. The number of DGGE sequences obtained per site was variable and, therefore, a high-throughput method was later employed to improve the diversity coverage. Consistent with previous surveys in Antarctica, both methods showed that filamentous cyanobacteria such as Leptolyngbya sp., Phormidium sp. and Microcoleus sp. were dominant in the studied sites. In addition, the studied localities differed in substrate type, climatic conditions and soil parameters, which likely resulted in differences in cyanobacterial community composition. Furthermore, the BSC growing on gneiss pebbles had lower cyanobacterial abundances than BSCs associated to granitic substrates. BELDIVA |
author2 |
CIP - Centre d'Ingénierie des Protéines - ULiège |
format |
Article in Journal/Newspaper |
author |
Pushkareva, Ekaterina Pessi, Igor S Namsaraev, Zorigto Mano, Marie-José Elster, Josef Wilmotte, Annick |
author_facet |
Pushkareva, Ekaterina Pessi, Igor S Namsaraev, Zorigto Mano, Marie-José Elster, Josef Wilmotte, Annick |
author_sort |
Pushkareva, Ekaterina |
title |
Cyanobacteria inhabiting biological soil crusts of a polar desert: Sør Rondane Mountains, Antarctica |
title_short |
Cyanobacteria inhabiting biological soil crusts of a polar desert: Sør Rondane Mountains, Antarctica |
title_full |
Cyanobacteria inhabiting biological soil crusts of a polar desert: Sør Rondane Mountains, Antarctica |
title_fullStr |
Cyanobacteria inhabiting biological soil crusts of a polar desert: Sør Rondane Mountains, Antarctica |
title_full_unstemmed |
Cyanobacteria inhabiting biological soil crusts of a polar desert: Sør Rondane Mountains, Antarctica |
title_sort |
cyanobacteria inhabiting biological soil crusts of a polar desert: sør rondane mountains, antarctica |
publisher |
Elsevier |
publishDate |
2018 |
url |
https://orbi.uliege.be/handle/2268/218320 https://doi.org/10.1016/j.syapm.2018.01.006 |
long_lat |
ENVELOPE(25.000,25.000,-72.000,-72.000) ENVELOPE(25.000,25.000,-72.000,-72.000) ENVELOPE(23.342,23.342,-71.955,-71.955) |
geographic |
Sør Rondane Mountains Sør-Rondane Utsteinen |
geographic_facet |
Sør Rondane Mountains Sør-Rondane Utsteinen |
genre |
Antarc* Antarctica polar desert |
genre_facet |
Antarc* Antarctica polar desert |
op_source |
Systematic and Applied Microbiology, 41, 363-373 (2018) |
op_relation |
https://www.sciencedirect.com/science/article/pii/S0723202018300274 urn:issn:0723-2020 urn:issn:1618-0984 https://orbi.uliege.be/handle/2268/218320 info:hdl:2268/218320 doi:10.1016/j.syapm.2018.01.006 info:pmid:29452715 |
op_rights |
restricted access http://purl.org/coar/access_right/c_16ec info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.syapm.2018.01.006 |
container_title |
Systematic and Applied Microbiology |
container_volume |
41 |
container_issue |
4 |
container_start_page |
363 |
op_container_end_page |
373 |
_version_ |
1812819200738066432 |