Individual Monitoring of Immune Response in Atlantic Salmon Salmo salar following Experimental Infection with Infectious Salmon Anaemia Virus (ISAV).
peer reviewed Monitoring the immune response in fish over the progression of a disease is traditionally carried out by experimental infection whereby animals are killed at regular intervals and samples taken. We describe here a novel approach to infectiology for salmonid fish where blood samples are...
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Online Access: | https://orbi.uliege.be/handle/2268/205288 https://orbi.uliege.be/bitstream/2268/205288/1/journal.pone.0137767.pdf https://doi.org/10.1371/journal.pone.0137767 |
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ftorbi:oai:orbi.ulg.ac.be:2268/205288 2024-04-21T07:57:19+00:00 Individual Monitoring of Immune Response in Atlantic Salmon Salmo salar following Experimental Infection with Infectious Salmon Anaemia Virus (ISAV). Collet, Bertrand Urquhart, Katy Mira Monte, Milena Collins, Catherine Garcia Perez, Sandro Secombes, Chris J. Hall, Malcolm 2015 https://orbi.uliege.be/handle/2268/205288 https://orbi.uliege.be/bitstream/2268/205288/1/journal.pone.0137767.pdf https://doi.org/10.1371/journal.pone.0137767 en eng Public Library of Science urn:issn:1932-6203 https://orbi.uliege.be/handle/2268/205288 info:hdl:2268/205288 https://orbi.uliege.be/bitstream/2268/205288/1/journal.pone.0137767.pdf doi:10.1371/journal.pone.0137767 scopus-id:2-s2.0-84946949492 info:pmid:26397117 open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess PLoS ONE, 10 (9), e0137767 (2015) Animals Biomarkers Fish Diseases/immunology/virology Hematocrit Immunity Isavirus/physiology Orthomyxoviridae Infections/immunology/veterinary/virology Salmo salar/immunology/virology Life sciences Veterinary medicine & animal health Sciences du vivant Médecine vétérinaire & santé animale journal article http://purl.org/coar/resource_type/c_6501 info:eu-repo/semantics/article peer reviewed 2015 ftorbi https://doi.org/10.1371/journal.pone.0137767 2024-03-27T14:52:59Z peer reviewed Monitoring the immune response in fish over the progression of a disease is traditionally carried out by experimental infection whereby animals are killed at regular intervals and samples taken. We describe here a novel approach to infectiology for salmonid fish where blood samples are collected repeatedly in a small group of PIT-tagged animals. This approach contributes to the reduction of animals used in research and to improved data quality. Two groups of 12 PIT-tagged Atlantic salmon (Salmo salar) were i.p infected with Infectious Salmon Anaemia Virus (ISAV) or culture medium and placed in 1 m3 tanks. Blood samples were collected at 0, 4, 8, 12, 16, 21 and 25 days post infection. The viral load, immune and stress response were determined in individual fish by real-time quantitative PCR (QPCR) on the blood cells, as well as the haematocrit used as an indicator of haemolysis, a clinical consequence of ISAV infection. "In-tank" anaesthesia was used in order to reduce the stress related to chase and netting prior to sampling. The data were analysed using a statistical approach which is novel with respect to its use in fish immunology. The repeated blood collection procedure did not induce stress response as measured by HSP70 and HSP90 gene expression in the un-infected animals. A strong increase in viraemia as well as a significant induction of Mx and gammaIP gene expression were observed in the infected group. Interleukin 10 was found induced at the later stage of the infection whereas no induction of CD8 or gamma IFN could be detected. These results and the advantages of this approach are discussed. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Liège: ORBi (Open Repository and Bibliography) PLOS ONE 10 9 e0137767 |
institution |
Open Polar |
collection |
University of Liège: ORBi (Open Repository and Bibliography) |
op_collection_id |
ftorbi |
language |
English |
topic |
Animals Biomarkers Fish Diseases/immunology/virology Hematocrit Immunity Isavirus/physiology Orthomyxoviridae Infections/immunology/veterinary/virology Salmo salar/immunology/virology Life sciences Veterinary medicine & animal health Sciences du vivant Médecine vétérinaire & santé animale |
spellingShingle |
Animals Biomarkers Fish Diseases/immunology/virology Hematocrit Immunity Isavirus/physiology Orthomyxoviridae Infections/immunology/veterinary/virology Salmo salar/immunology/virology Life sciences Veterinary medicine & animal health Sciences du vivant Médecine vétérinaire & santé animale Collet, Bertrand Urquhart, Katy Mira Monte, Milena Collins, Catherine Garcia Perez, Sandro Secombes, Chris J. Hall, Malcolm Individual Monitoring of Immune Response in Atlantic Salmon Salmo salar following Experimental Infection with Infectious Salmon Anaemia Virus (ISAV). |
topic_facet |
Animals Biomarkers Fish Diseases/immunology/virology Hematocrit Immunity Isavirus/physiology Orthomyxoviridae Infections/immunology/veterinary/virology Salmo salar/immunology/virology Life sciences Veterinary medicine & animal health Sciences du vivant Médecine vétérinaire & santé animale |
description |
peer reviewed Monitoring the immune response in fish over the progression of a disease is traditionally carried out by experimental infection whereby animals are killed at regular intervals and samples taken. We describe here a novel approach to infectiology for salmonid fish where blood samples are collected repeatedly in a small group of PIT-tagged animals. This approach contributes to the reduction of animals used in research and to improved data quality. Two groups of 12 PIT-tagged Atlantic salmon (Salmo salar) were i.p infected with Infectious Salmon Anaemia Virus (ISAV) or culture medium and placed in 1 m3 tanks. Blood samples were collected at 0, 4, 8, 12, 16, 21 and 25 days post infection. The viral load, immune and stress response were determined in individual fish by real-time quantitative PCR (QPCR) on the blood cells, as well as the haematocrit used as an indicator of haemolysis, a clinical consequence of ISAV infection. "In-tank" anaesthesia was used in order to reduce the stress related to chase and netting prior to sampling. The data were analysed using a statistical approach which is novel with respect to its use in fish immunology. The repeated blood collection procedure did not induce stress response as measured by HSP70 and HSP90 gene expression in the un-infected animals. A strong increase in viraemia as well as a significant induction of Mx and gammaIP gene expression were observed in the infected group. Interleukin 10 was found induced at the later stage of the infection whereas no induction of CD8 or gamma IFN could be detected. These results and the advantages of this approach are discussed. |
format |
Article in Journal/Newspaper |
author |
Collet, Bertrand Urquhart, Katy Mira Monte, Milena Collins, Catherine Garcia Perez, Sandro Secombes, Chris J. Hall, Malcolm |
author_facet |
Collet, Bertrand Urquhart, Katy Mira Monte, Milena Collins, Catherine Garcia Perez, Sandro Secombes, Chris J. Hall, Malcolm |
author_sort |
Collet, Bertrand |
title |
Individual Monitoring of Immune Response in Atlantic Salmon Salmo salar following Experimental Infection with Infectious Salmon Anaemia Virus (ISAV). |
title_short |
Individual Monitoring of Immune Response in Atlantic Salmon Salmo salar following Experimental Infection with Infectious Salmon Anaemia Virus (ISAV). |
title_full |
Individual Monitoring of Immune Response in Atlantic Salmon Salmo salar following Experimental Infection with Infectious Salmon Anaemia Virus (ISAV). |
title_fullStr |
Individual Monitoring of Immune Response in Atlantic Salmon Salmo salar following Experimental Infection with Infectious Salmon Anaemia Virus (ISAV). |
title_full_unstemmed |
Individual Monitoring of Immune Response in Atlantic Salmon Salmo salar following Experimental Infection with Infectious Salmon Anaemia Virus (ISAV). |
title_sort |
individual monitoring of immune response in atlantic salmon salmo salar following experimental infection with infectious salmon anaemia virus (isav). |
publisher |
Public Library of Science |
publishDate |
2015 |
url |
https://orbi.uliege.be/handle/2268/205288 https://orbi.uliege.be/bitstream/2268/205288/1/journal.pone.0137767.pdf https://doi.org/10.1371/journal.pone.0137767 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
PLoS ONE, 10 (9), e0137767 (2015) |
op_relation |
urn:issn:1932-6203 https://orbi.uliege.be/handle/2268/205288 info:hdl:2268/205288 https://orbi.uliege.be/bitstream/2268/205288/1/journal.pone.0137767.pdf doi:10.1371/journal.pone.0137767 scopus-id:2-s2.0-84946949492 info:pmid:26397117 |
op_rights |
open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0137767 |
container_title |
PLOS ONE |
container_volume |
10 |
container_issue |
9 |
container_start_page |
e0137767 |
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1796939006419664896 |