Absence of selenium protection against methylmercury toxicity in harbour seal leucocytes in vitro
peer reviewed Previous studies described high concentrations of mercury (Hg) and selenium (Se) in the blood of harbour seals, Phoca vitulina from the North Sea. In the present study, we evaluated the in vitro potential protective effects of sodium selenite (Na2SeO3) and selenomethionine (SeMet) on c...
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Online Access: | https://orbi.uliege.be/handle/2268/196892 https://doi.org/10.1016/j.marpolbul.2016.04.060 |
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ftorbi:oai:orbi.ulg.ac.be:2268/196892 2024-04-21T08:04:13+00:00 Absence of selenium protection against methylmercury toxicity in harbour seal leucocytes in vitro Das, Krishna Dupont, Aurélie De Pauw-Gillet, Marie-Claire Debier, Cathy Siebert, Ursula MARE - Centre Interfacultaire de Recherches en Océanologie - ULiège 2016 https://orbi.uliege.be/handle/2268/196892 https://doi.org/10.1016/j.marpolbul.2016.04.060 en eng Pergamon http://dx.doi.org/10.1016/j.marpolbul.2016.04.060 urn:issn:0025-326X https://orbi.uliege.be/handle/2268/196892 info:hdl:2268/196892 doi:10.1016/j.marpolbul.2016.04.060 scopus-id:2-s2.0-84966800352 info:pmid:27197766 restricted access http://purl.org/coar/access_right/c_16ec info:eu-repo/semantics/restrictedAccess Marine Pollution Bulletin, 108, 70-76 (2016) marine mammals pollution mercury selenium immune system Life sciences Aquatic sciences & oceanology Environmental sciences & ecology Sciences du vivant Sciences aquatiques & océanologie Sciences de l’environnement & écologie journal article http://purl.org/coar/resource_type/c_6501 info:eu-repo/semantics/article peer reviewed 2016 ftorbi https://doi.org/10.1016/j.marpolbul.2016.04.060 2024-03-27T14:52:54Z peer reviewed Previous studies described high concentrations of mercury (Hg) and selenium (Se) in the blood of harbour seals, Phoca vitulina from the North Sea. In the present study, we evaluated the in vitro potential protective effects of sodium selenite (Na2SeO3) and selenomethionine (SeMet) on cell proliferation of harbour seal lymphocytes exposed to MeHgCl 0.75 µM. In vitro exposure of ConA-stimulated T lymphocytes resulted in severe inhibition of DNA synthesis, likely linked to severe loss of mitochondrial membrane potential at 0.75 µM. Neither selenite nor SeMet showed a protective effect against MeHg toxicity expressed at the T lymphocyte proliferation level for harbour seals. Selenite and SeMet did not show negative effects regarding lymphocyte proliferation and mitochondrial membrane potential. To conclude, our results clearly demonstrated that MeHg affected in vitro immune cells exposure with no protective effects of selenium at a molar ratio Hg:Se of 1:10 in harbour seals from the North Sea. Article in Journal/Newspaper harbour seal Phoca vitulina University of Liège: ORBi (Open Repository and Bibliography) Marine Pollution Bulletin 108 1-2 70 76 |
institution |
Open Polar |
collection |
University of Liège: ORBi (Open Repository and Bibliography) |
op_collection_id |
ftorbi |
language |
English |
topic |
marine mammals pollution mercury selenium immune system Life sciences Aquatic sciences & oceanology Environmental sciences & ecology Sciences du vivant Sciences aquatiques & océanologie Sciences de l’environnement & écologie |
spellingShingle |
marine mammals pollution mercury selenium immune system Life sciences Aquatic sciences & oceanology Environmental sciences & ecology Sciences du vivant Sciences aquatiques & océanologie Sciences de l’environnement & écologie Das, Krishna Dupont, Aurélie De Pauw-Gillet, Marie-Claire Debier, Cathy Siebert, Ursula Absence of selenium protection against methylmercury toxicity in harbour seal leucocytes in vitro |
topic_facet |
marine mammals pollution mercury selenium immune system Life sciences Aquatic sciences & oceanology Environmental sciences & ecology Sciences du vivant Sciences aquatiques & océanologie Sciences de l’environnement & écologie |
description |
peer reviewed Previous studies described high concentrations of mercury (Hg) and selenium (Se) in the blood of harbour seals, Phoca vitulina from the North Sea. In the present study, we evaluated the in vitro potential protective effects of sodium selenite (Na2SeO3) and selenomethionine (SeMet) on cell proliferation of harbour seal lymphocytes exposed to MeHgCl 0.75 µM. In vitro exposure of ConA-stimulated T lymphocytes resulted in severe inhibition of DNA synthesis, likely linked to severe loss of mitochondrial membrane potential at 0.75 µM. Neither selenite nor SeMet showed a protective effect against MeHg toxicity expressed at the T lymphocyte proliferation level for harbour seals. Selenite and SeMet did not show negative effects regarding lymphocyte proliferation and mitochondrial membrane potential. To conclude, our results clearly demonstrated that MeHg affected in vitro immune cells exposure with no protective effects of selenium at a molar ratio Hg:Se of 1:10 in harbour seals from the North Sea. |
author2 |
MARE - Centre Interfacultaire de Recherches en Océanologie - ULiège |
format |
Article in Journal/Newspaper |
author |
Das, Krishna Dupont, Aurélie De Pauw-Gillet, Marie-Claire Debier, Cathy Siebert, Ursula |
author_facet |
Das, Krishna Dupont, Aurélie De Pauw-Gillet, Marie-Claire Debier, Cathy Siebert, Ursula |
author_sort |
Das, Krishna |
title |
Absence of selenium protection against methylmercury toxicity in harbour seal leucocytes in vitro |
title_short |
Absence of selenium protection against methylmercury toxicity in harbour seal leucocytes in vitro |
title_full |
Absence of selenium protection against methylmercury toxicity in harbour seal leucocytes in vitro |
title_fullStr |
Absence of selenium protection against methylmercury toxicity in harbour seal leucocytes in vitro |
title_full_unstemmed |
Absence of selenium protection against methylmercury toxicity in harbour seal leucocytes in vitro |
title_sort |
absence of selenium protection against methylmercury toxicity in harbour seal leucocytes in vitro |
publisher |
Pergamon |
publishDate |
2016 |
url |
https://orbi.uliege.be/handle/2268/196892 https://doi.org/10.1016/j.marpolbul.2016.04.060 |
genre |
harbour seal Phoca vitulina |
genre_facet |
harbour seal Phoca vitulina |
op_source |
Marine Pollution Bulletin, 108, 70-76 (2016) |
op_relation |
http://dx.doi.org/10.1016/j.marpolbul.2016.04.060 urn:issn:0025-326X https://orbi.uliege.be/handle/2268/196892 info:hdl:2268/196892 doi:10.1016/j.marpolbul.2016.04.060 scopus-id:2-s2.0-84966800352 info:pmid:27197766 |
op_rights |
restricted access http://purl.org/coar/access_right/c_16ec info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.marpolbul.2016.04.060 |
container_title |
Marine Pollution Bulletin |
container_volume |
108 |
container_issue |
1-2 |
container_start_page |
70 |
op_container_end_page |
76 |
_version_ |
1796943865315328000 |