BIOGEOGRAPHIC PATTERNS IN ANTARCTIC LACUSTRINE PROKARYOTES
Amplified climate change, increased human activity and the introduction of alien species likely form the biggest threat to Antarctic terrestrial ecosystems through range size expansions and contractions, regional extirpation and impacts on ecosystem functions. Despite their crucial role in the funct...
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ftorbi:oai:orbi.ulg.ac.be:2268/190976 2024-04-21T07:52:39+00:00 BIOGEOGRAPHIC PATTERNS IN ANTARCTIC LACUSTRINE PROKARYOTES Tytgat, Bjorn Verleyen, Elie Sweetlove, Maxime Obbels, Dagmar D'hondt, Sofie Wilmotte, Annick Vyverman, Wilm Willems, Anne CIP - Centre d'Ingénierie des Protéines - ULiège 2015-09-07 A0 https://orbi.uliege.be/handle/2268/190976 en eng http://polaralpinemicrobiology2015.prf.jcu.cz/data/uploads/pam_2015_boa_small.pdf https://orbi.uliege.be/handle/2268/190976 info:hdl:2268/190976 6th Symposium on Polar and Alpine Microbiology, Ceske Budejovice, Czechia [CZ], 6 septembre au 9 septembre 2015 Antarctica microbial mats biogeography Next Generation Sequencing Life sciences Environmental sciences & ecology Microbiology Sciences du vivant Sciences de l’environnement & écologie Microbiologie conference poster not in proceedings http://purl.org/coar/resource_type/c_18co info:eu-repo/semantics/conferencePoster 2015 ftorbi 2024-03-27T14:47:03Z Amplified climate change, increased human activity and the introduction of alien species likely form the biggest threat to Antarctic terrestrial ecosystems through range size expansions and contractions, regional extirpation and impacts on ecosystem functions. Despite their crucial role in the functioning of Antarctic terrestrial ecosystems , little is known about the present -day diversity and biogeography of microorganisms such as prokaryotes and microeukaryotes in the Antarctic Biogeographic Realm. Furthermore, identification of the key processes underlying microbial biodiversity dynamics is essential to understand and predict the consequences of global change on Antarctic lacustrine ecosystems. We analysed bacterial biodiversity in a total of 152 lacustrine microbial mat samples, distributed over the three main Biogeographic regions in the Antarctic Realm, including continental Antarctica, Maritime Antarctica and the Sub-Antarctic Islands comprising the southern Indian Ocean Province (SIOP) and the southern Pacific Ocean Province (SPOP). We targeted the V1-V3 variable regions of the 16S rRNA gene. Amplicon sequencing was done on an Illumina PE300 MiSeq. Sequences were processed using Usearch and Uparse, Mothur and custom scripts for basic parsing. An OTU cut-off was defined at 97 % sequence similarity, and sequences were mapped against a local GreenGenes database. Downstream analyses were performed using several R packages. We obtained about three million high quality sequences, with an average length of 500 bp. Sequences belonged to 8237 OTUs, and were distributed over 51 phyla and 366 genera. In addition, 649 OTUs remained unclassified at the phylum level and 6263 at the genus level. Mean OTU richness differed strongly between the four biogeographic regions. The lakes from Maritime Antarctica had a higher richness than those from Continental Antarctica. Interestingly, in sub-Antarctica OTU richness was strongly variable, with Marion Island (SIOP) having the lowest and Macquarie Island (SPOP) having on ... Conference Object Antarc* Antarctic Antarctica Macquarie Island Marion Island University of Liège: ORBi (Open Repository and Bibliography) |
institution |
Open Polar |
collection |
University of Liège: ORBi (Open Repository and Bibliography) |
op_collection_id |
ftorbi |
language |
English |
topic |
Antarctica microbial mats biogeography Next Generation Sequencing Life sciences Environmental sciences & ecology Microbiology Sciences du vivant Sciences de l’environnement & écologie Microbiologie |
spellingShingle |
Antarctica microbial mats biogeography Next Generation Sequencing Life sciences Environmental sciences & ecology Microbiology Sciences du vivant Sciences de l’environnement & écologie Microbiologie Tytgat, Bjorn Verleyen, Elie Sweetlove, Maxime Obbels, Dagmar D'hondt, Sofie Wilmotte, Annick Vyverman, Wilm Willems, Anne BIOGEOGRAPHIC PATTERNS IN ANTARCTIC LACUSTRINE PROKARYOTES |
topic_facet |
Antarctica microbial mats biogeography Next Generation Sequencing Life sciences Environmental sciences & ecology Microbiology Sciences du vivant Sciences de l’environnement & écologie Microbiologie |
description |
Amplified climate change, increased human activity and the introduction of alien species likely form the biggest threat to Antarctic terrestrial ecosystems through range size expansions and contractions, regional extirpation and impacts on ecosystem functions. Despite their crucial role in the functioning of Antarctic terrestrial ecosystems , little is known about the present -day diversity and biogeography of microorganisms such as prokaryotes and microeukaryotes in the Antarctic Biogeographic Realm. Furthermore, identification of the key processes underlying microbial biodiversity dynamics is essential to understand and predict the consequences of global change on Antarctic lacustrine ecosystems. We analysed bacterial biodiversity in a total of 152 lacustrine microbial mat samples, distributed over the three main Biogeographic regions in the Antarctic Realm, including continental Antarctica, Maritime Antarctica and the Sub-Antarctic Islands comprising the southern Indian Ocean Province (SIOP) and the southern Pacific Ocean Province (SPOP). We targeted the V1-V3 variable regions of the 16S rRNA gene. Amplicon sequencing was done on an Illumina PE300 MiSeq. Sequences were processed using Usearch and Uparse, Mothur and custom scripts for basic parsing. An OTU cut-off was defined at 97 % sequence similarity, and sequences were mapped against a local GreenGenes database. Downstream analyses were performed using several R packages. We obtained about three million high quality sequences, with an average length of 500 bp. Sequences belonged to 8237 OTUs, and were distributed over 51 phyla and 366 genera. In addition, 649 OTUs remained unclassified at the phylum level and 6263 at the genus level. Mean OTU richness differed strongly between the four biogeographic regions. The lakes from Maritime Antarctica had a higher richness than those from Continental Antarctica. Interestingly, in sub-Antarctica OTU richness was strongly variable, with Marion Island (SIOP) having the lowest and Macquarie Island (SPOP) having on ... |
author2 |
CIP - Centre d'Ingénierie des Protéines - ULiège |
format |
Conference Object |
author |
Tytgat, Bjorn Verleyen, Elie Sweetlove, Maxime Obbels, Dagmar D'hondt, Sofie Wilmotte, Annick Vyverman, Wilm Willems, Anne |
author_facet |
Tytgat, Bjorn Verleyen, Elie Sweetlove, Maxime Obbels, Dagmar D'hondt, Sofie Wilmotte, Annick Vyverman, Wilm Willems, Anne |
author_sort |
Tytgat, Bjorn |
title |
BIOGEOGRAPHIC PATTERNS IN ANTARCTIC LACUSTRINE PROKARYOTES |
title_short |
BIOGEOGRAPHIC PATTERNS IN ANTARCTIC LACUSTRINE PROKARYOTES |
title_full |
BIOGEOGRAPHIC PATTERNS IN ANTARCTIC LACUSTRINE PROKARYOTES |
title_fullStr |
BIOGEOGRAPHIC PATTERNS IN ANTARCTIC LACUSTRINE PROKARYOTES |
title_full_unstemmed |
BIOGEOGRAPHIC PATTERNS IN ANTARCTIC LACUSTRINE PROKARYOTES |
title_sort |
biogeographic patterns in antarctic lacustrine prokaryotes |
publishDate |
2015 |
url |
https://orbi.uliege.be/handle/2268/190976 |
genre |
Antarc* Antarctic Antarctica Macquarie Island Marion Island |
genre_facet |
Antarc* Antarctic Antarctica Macquarie Island Marion Island |
op_source |
6th Symposium on Polar and Alpine Microbiology, Ceske Budejovice, Czechia [CZ], 6 septembre au 9 septembre 2015 |
op_relation |
http://polaralpinemicrobiology2015.prf.jcu.cz/data/uploads/pam_2015_boa_small.pdf https://orbi.uliege.be/handle/2268/190976 info:hdl:2268/190976 |
_version_ |
1796935855446687744 |