Effects of Methylmercury on Harbour Seal Peripheral Blood Leucocytes In Vitro Studied by Electron Microscopy
peer reviewed Methylmercury MeHg is highly immunotoxic and can alter the health status of the harbour seal, Phoca vitulina, from the North Sea. To investigate the mechanism of MeHg-induced toxicity in harbour seal lymphocytes, ConA-stimulated peripheral blood leucocytes were exposed in vitro to sub-...
Published in: | Archives of Environmental Contamination and Toxicology |
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Springer Science & Business Media B.V.
2016
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Online Access: | https://orbi.uliege.be/handle/2268/184665 https://doi.org/10.1007/s00244-015-0207-y |
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ftorbi:oai:orbi.ulg.ac.be:2268/184665 2024-04-21T08:04:13+00:00 Effects of Methylmercury on Harbour Seal Peripheral Blood Leucocytes In Vitro Studied by Electron Microscopy Dupont, Aurélie De Pauw-Gillet, Marie-Claire Schnitzler, Joseph Siebert, Ursula Das, Krishna MARE - Centre Interfacultaire de Recherches en Océanologie - ULiège 2016-01 https://orbi.uliege.be/handle/2268/184665 https://doi.org/10.1007/s00244-015-0207-y en eng Springer Science & Business Media B.V. http://reflexions.ulg.ac.be/Phoques http://reflexions.ulg.ac.be/en/Seals urn:issn:0090-4341 urn:issn:1432-0703 https://orbi.uliege.be/handle/2268/184665 info:hdl:2268/184665 doi:10.1007/s00244-015-0207-y scopus-id:2-s2.0-84958116453 info:pmid:26264045 restricted access http://purl.org/coar/access_right/c_16ec info:eu-repo/semantics/restrictedAccess Archives of Environmental Contamination and Toxicology, 70 (1), 133-142 (2016-01) marine mammals harbour seal Phoca vitulina methylmercury blood lymphocytes in vitro exposure Life sciences Environmental sciences & ecology Aquatic sciences & oceanology Sciences du vivant Sciences de l’environnement & écologie Sciences aquatiques & océanologie journal article http://purl.org/coar/resource_type/c_6501 info:eu-repo/semantics/article peer reviewed 2016 ftorbi https://doi.org/10.1007/s00244-015-0207-y 2024-03-27T14:52:59Z peer reviewed Methylmercury MeHg is highly immunotoxic and can alter the health status of the harbour seal, Phoca vitulina, from the North Sea. To investigate the mechanism of MeHg-induced toxicity in harbour seal lymphocytes, ConA-stimulated peripheral blood leucocytes were exposed in vitro to sub-lethal concentrations of MeHgCl (0.2, 1 and 2 µM) for 72h, and then analysed for their viability and ultrastructure. After 72h of incubation, cells were counted with a propidium iodide staining technique, a metabolic MTS assay was performed, and cells exposed to 1 µM MeHgCl were observed by transmission electron microscopy (TEM). Alive cell numbers decreased with increased MeHgCl concentrations. In presence of ConA and 1µM of MeHgCl, TEM images revealed a higher frequency of apoptotic cells. Exposed cells displayed condensation of the chromatin at the nuclear membrane and mitochondrial damages. The results suggest that in vitro MeHgCl induced apoptosis in harbour seal lymphocytes through a mitochondrial pathway. Article in Journal/Newspaper harbour seal Phoca vitulina University of Liège: ORBi (Open Repository and Bibliography) Archives of Environmental Contamination and Toxicology 70 1 133 142 |
institution |
Open Polar |
collection |
University of Liège: ORBi (Open Repository and Bibliography) |
op_collection_id |
ftorbi |
language |
English |
topic |
marine mammals harbour seal Phoca vitulina methylmercury blood lymphocytes in vitro exposure Life sciences Environmental sciences & ecology Aquatic sciences & oceanology Sciences du vivant Sciences de l’environnement & écologie Sciences aquatiques & océanologie |
spellingShingle |
marine mammals harbour seal Phoca vitulina methylmercury blood lymphocytes in vitro exposure Life sciences Environmental sciences & ecology Aquatic sciences & oceanology Sciences du vivant Sciences de l’environnement & écologie Sciences aquatiques & océanologie Dupont, Aurélie De Pauw-Gillet, Marie-Claire Schnitzler, Joseph Siebert, Ursula Das, Krishna Effects of Methylmercury on Harbour Seal Peripheral Blood Leucocytes In Vitro Studied by Electron Microscopy |
topic_facet |
marine mammals harbour seal Phoca vitulina methylmercury blood lymphocytes in vitro exposure Life sciences Environmental sciences & ecology Aquatic sciences & oceanology Sciences du vivant Sciences de l’environnement & écologie Sciences aquatiques & océanologie |
description |
peer reviewed Methylmercury MeHg is highly immunotoxic and can alter the health status of the harbour seal, Phoca vitulina, from the North Sea. To investigate the mechanism of MeHg-induced toxicity in harbour seal lymphocytes, ConA-stimulated peripheral blood leucocytes were exposed in vitro to sub-lethal concentrations of MeHgCl (0.2, 1 and 2 µM) for 72h, and then analysed for their viability and ultrastructure. After 72h of incubation, cells were counted with a propidium iodide staining technique, a metabolic MTS assay was performed, and cells exposed to 1 µM MeHgCl were observed by transmission electron microscopy (TEM). Alive cell numbers decreased with increased MeHgCl concentrations. In presence of ConA and 1µM of MeHgCl, TEM images revealed a higher frequency of apoptotic cells. Exposed cells displayed condensation of the chromatin at the nuclear membrane and mitochondrial damages. The results suggest that in vitro MeHgCl induced apoptosis in harbour seal lymphocytes through a mitochondrial pathway. |
author2 |
MARE - Centre Interfacultaire de Recherches en Océanologie - ULiège |
format |
Article in Journal/Newspaper |
author |
Dupont, Aurélie De Pauw-Gillet, Marie-Claire Schnitzler, Joseph Siebert, Ursula Das, Krishna |
author_facet |
Dupont, Aurélie De Pauw-Gillet, Marie-Claire Schnitzler, Joseph Siebert, Ursula Das, Krishna |
author_sort |
Dupont, Aurélie |
title |
Effects of Methylmercury on Harbour Seal Peripheral Blood Leucocytes In Vitro Studied by Electron Microscopy |
title_short |
Effects of Methylmercury on Harbour Seal Peripheral Blood Leucocytes In Vitro Studied by Electron Microscopy |
title_full |
Effects of Methylmercury on Harbour Seal Peripheral Blood Leucocytes In Vitro Studied by Electron Microscopy |
title_fullStr |
Effects of Methylmercury on Harbour Seal Peripheral Blood Leucocytes In Vitro Studied by Electron Microscopy |
title_full_unstemmed |
Effects of Methylmercury on Harbour Seal Peripheral Blood Leucocytes In Vitro Studied by Electron Microscopy |
title_sort |
effects of methylmercury on harbour seal peripheral blood leucocytes in vitro studied by electron microscopy |
publisher |
Springer Science & Business Media B.V. |
publishDate |
2016 |
url |
https://orbi.uliege.be/handle/2268/184665 https://doi.org/10.1007/s00244-015-0207-y |
genre |
harbour seal Phoca vitulina |
genre_facet |
harbour seal Phoca vitulina |
op_source |
Archives of Environmental Contamination and Toxicology, 70 (1), 133-142 (2016-01) |
op_relation |
http://reflexions.ulg.ac.be/Phoques http://reflexions.ulg.ac.be/en/Seals urn:issn:0090-4341 urn:issn:1432-0703 https://orbi.uliege.be/handle/2268/184665 info:hdl:2268/184665 doi:10.1007/s00244-015-0207-y scopus-id:2-s2.0-84958116453 info:pmid:26264045 |
op_rights |
restricted access http://purl.org/coar/access_right/c_16ec info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1007/s00244-015-0207-y |
container_title |
Archives of Environmental Contamination and Toxicology |
container_volume |
70 |
container_issue |
1 |
container_start_page |
133 |
op_container_end_page |
142 |
_version_ |
1796943857758240768 |