Molecular diversity of microorganisms in Antarctic lacustrine microbial mats
The BeSPO project CCAMBIO aims to study the biogeographical distribution of microorganisms in lacustrine microbial mats using a combination of techniques including microscopical observations (light and electronic), strain isolation, and molecular diversity assessment using Next Generation Sequencing...
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ftorbi:oai:orbi.ulg.ac.be:2268/179919 2024-04-21T07:48:54+00:00 Molecular diversity of microorganisms in Antarctic lacustrine microbial mats Wilmotte, Annick Stelmach Pessi, Igor Sweetlove, Maxime Obbels, Dagmar Vanormelingen, Pieter Tytgat, Bjorn Willems, Anne Verleyen, Elie Vyverman, Wim Van De Vijver, Bart Lara, Yannick Van De Putte, Anton CIP - Centre d'Ingénierie des Protéines - ULiège 2015-02 1 https://orbi.uliege.be/handle/2268/179919 en eng Program Book of 2015 Aquatic Sciences Meeting of ASLO https://orbi.uliege.be/handle/2268/179919 info:hdl:2268/179919 2015 Aquatic Sciences Meeting 'Aquatic Sciences: global and regional perspectives - North meets South', Grenada, Spain [ES], 22-27 February 2015 Biogeography Antarctica benthic mats Life sciences Environmental sciences & ecology Microbiology Sciences du vivant Sciences de l’environnement & écologie Microbiologie conference paper not in proceedings http://purl.org/coar/resource_type/c_18cp info:eu-repo/semantics/conferencePaper 2015 ftorbi 2024-03-27T14:46:29Z The BeSPO project CCAMBIO aims to study the biogeographical distribution of microorganisms in lacustrine microbial mats using a combination of techniques including microscopical observations (light and electronic), strain isolation, and molecular diversity assessment using Next Generation Sequencing. The samples were collected in different Antarctic and sub-Antarctic biogeographical regions. A multivariate analysis of diatoms showed that these regions hosted different diatom flora. Endemic diatom taxa were also observed, and a multigene molecular phylogeny of Pinnularia borealis showed a high genetic diversity. A new Scenedesmacean species was described from Antarctica, Chodatodesmus australis. A comparison of the bacterial diversity retrieved by cultivation or NGS showed a complementarity of both approaches and differences when different variable regions of the 16S rRNA gene were used. Novel and unclassified sequences, also observed by other authors, were obtained. Pilot studies were conducted for the microeukaryotes and cyanobacteria to select NGS protocols and bioinformatic pipelines. The purpose is to deposit the diversity data in the “Microbial Antarctic Resource System (MARS)” presently developed into the webportal ‘biodiversity.aq’. CCAMBIO Conference Object Antarc* Antarctic Antarctica University of Liège: ORBi (Open Repository and Bibliography) |
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Open Polar |
collection |
University of Liège: ORBi (Open Repository and Bibliography) |
op_collection_id |
ftorbi |
language |
English |
topic |
Biogeography Antarctica benthic mats Life sciences Environmental sciences & ecology Microbiology Sciences du vivant Sciences de l’environnement & écologie Microbiologie |
spellingShingle |
Biogeography Antarctica benthic mats Life sciences Environmental sciences & ecology Microbiology Sciences du vivant Sciences de l’environnement & écologie Microbiologie Wilmotte, Annick Stelmach Pessi, Igor Sweetlove, Maxime Obbels, Dagmar Vanormelingen, Pieter Tytgat, Bjorn Willems, Anne Verleyen, Elie Vyverman, Wim Van De Vijver, Bart Lara, Yannick Van De Putte, Anton Molecular diversity of microorganisms in Antarctic lacustrine microbial mats |
topic_facet |
Biogeography Antarctica benthic mats Life sciences Environmental sciences & ecology Microbiology Sciences du vivant Sciences de l’environnement & écologie Microbiologie |
description |
The BeSPO project CCAMBIO aims to study the biogeographical distribution of microorganisms in lacustrine microbial mats using a combination of techniques including microscopical observations (light and electronic), strain isolation, and molecular diversity assessment using Next Generation Sequencing. The samples were collected in different Antarctic and sub-Antarctic biogeographical regions. A multivariate analysis of diatoms showed that these regions hosted different diatom flora. Endemic diatom taxa were also observed, and a multigene molecular phylogeny of Pinnularia borealis showed a high genetic diversity. A new Scenedesmacean species was described from Antarctica, Chodatodesmus australis. A comparison of the bacterial diversity retrieved by cultivation or NGS showed a complementarity of both approaches and differences when different variable regions of the 16S rRNA gene were used. Novel and unclassified sequences, also observed by other authors, were obtained. Pilot studies were conducted for the microeukaryotes and cyanobacteria to select NGS protocols and bioinformatic pipelines. The purpose is to deposit the diversity data in the “Microbial Antarctic Resource System (MARS)” presently developed into the webportal ‘biodiversity.aq’. CCAMBIO |
author2 |
CIP - Centre d'Ingénierie des Protéines - ULiège |
format |
Conference Object |
author |
Wilmotte, Annick Stelmach Pessi, Igor Sweetlove, Maxime Obbels, Dagmar Vanormelingen, Pieter Tytgat, Bjorn Willems, Anne Verleyen, Elie Vyverman, Wim Van De Vijver, Bart Lara, Yannick Van De Putte, Anton |
author_facet |
Wilmotte, Annick Stelmach Pessi, Igor Sweetlove, Maxime Obbels, Dagmar Vanormelingen, Pieter Tytgat, Bjorn Willems, Anne Verleyen, Elie Vyverman, Wim Van De Vijver, Bart Lara, Yannick Van De Putte, Anton |
author_sort |
Wilmotte, Annick |
title |
Molecular diversity of microorganisms in Antarctic lacustrine microbial mats |
title_short |
Molecular diversity of microorganisms in Antarctic lacustrine microbial mats |
title_full |
Molecular diversity of microorganisms in Antarctic lacustrine microbial mats |
title_fullStr |
Molecular diversity of microorganisms in Antarctic lacustrine microbial mats |
title_full_unstemmed |
Molecular diversity of microorganisms in Antarctic lacustrine microbial mats |
title_sort |
molecular diversity of microorganisms in antarctic lacustrine microbial mats |
publishDate |
2015 |
url |
https://orbi.uliege.be/handle/2268/179919 |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
2015 Aquatic Sciences Meeting 'Aquatic Sciences: global and regional perspectives - North meets South', Grenada, Spain [ES], 22-27 February 2015 |
op_relation |
Program Book of 2015 Aquatic Sciences Meeting of ASLO https://orbi.uliege.be/handle/2268/179919 info:hdl:2268/179919 |
_version_ |
1796952099403071488 |