Potent toxins in Arctic environments--presence of saxitoxins and an unusual microcystin variant in Arctic freshwater ecosystems.
peer reviewed Cyanobacteria are the predominant phototrophs in freshwater ecosystems of the polar regions where they commonly form extensive benthic mats. Despite their major biological role in these ecosystems, little attention has been paid to their physiology and biochemistry. An important featur...
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Language: | English |
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2013
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Online Access: | https://orbi.uliege.be/handle/2268/178154 https://doi.org/10.1016/j.cbi.2013.04.011 |
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ftorbi:oai:orbi.ulg.ac.be:2268/178154 2024-04-21T07:47:38+00:00 Potent toxins in Arctic environments--presence of saxitoxins and an unusual microcystin variant in Arctic freshwater ecosystems. Kleinteich, Julia Wood, Susanna A Puddick, Jonathan Schleheck, David Küpper, Frithjof C. Dietrich, Daniel 2013 https://orbi.uliege.be/handle/2268/178154 https://doi.org/10.1016/j.cbi.2013.04.011 en eng Elsevier Ireland Ltd http://www.ncbi.nlm.nih.gov/pubmed/23648386 urn:issn:0009-2797 urn:issn:1872-7786 https://orbi.uliege.be/handle/2268/178154 info:hdl:2268/178154 doi:10.1016/j.cbi.2013.04.011 scopus-id:2-s2.0-84888291241 info:pmid:23648386 restricted access http://purl.org/coar/access_right/c_16ec info:eu-repo/semantics/restrictedAccess Chemico-Biological Interactions, 206 (2), 423-31 (2013) 16S Arctic Regions Bacterial Proteins Bacterial Proteins: classification Bacterial Proteins: genetics Bacterial Proteins: metabolism Chromatography Cyanobacteria Cyanobacteria: genetics Cyanobacteria: isolation purification Cyanobacteria: metabolism Ecosystem Enzyme-Linked Immunosorbent Assay Fresh Water Fresh Water: chemistry Fresh Water: microbiology High Pressure Liquid Mass Spectrometry Messenger Messenger: metabolism Microcystins Microcystins: analysis Phylogeny RNA Ribosomal Saxitoxin Saxitoxin: analysis Life sciences Microbiology Sciences du vivant Microbiologie journal article http://purl.org/coar/resource_type/c_6501 info:eu-repo/semantics/article peer reviewed 2013 ftorbi https://doi.org/10.1016/j.cbi.2013.04.011 2024-03-27T14:53:59Z peer reviewed Cyanobacteria are the predominant phototrophs in freshwater ecosystems of the polar regions where they commonly form extensive benthic mats. Despite their major biological role in these ecosystems, little attention has been paid to their physiology and biochemistry. An important feature of cyanobacteria from the temperate and tropical regions is the production of a large variety of toxic secondary metabolites. In Antarctica, and more recently in the Arctic, the cyanobacterial toxins microcystin and nodularin (Antarctic only) have been detected in freshwater microbial mats. To date other cyanobacterial toxins have not been reported from these locations. Five Arctic cyanobacterial communities were screened for saxitoxin, another common cyanobacterial toxin, and microcystins using immunological, spectroscopic and molecular methods. Saxitoxin was detected for the first time in cyanobacteria from the Arctic. In addition, an unusual microcystin variant was identified using liquid chromatography-mass spectrometry. Gene expression analyses confirmed the analytical findings, whereby parts of the sxt and mcy operon involved in saxitoxin and microcystin synthesis, were detected and sequenced in one and five of the Arctic cyanobacterial samples, respectively. The detection of these compounds in the cryosphere improves the understanding of the biogeography and distribution of toxic cyanobacteria globally. The sequences of sxt and mcy genes provided from this habitat for the first time may help to clarify the evolutionary origin of toxin production in cyanobacteria. Article in Journal/Newspaper Antarc* Antarctic Antarctica University of Liège: ORBi (Open Repository and Bibliography) Chemico-Biological Interactions 206 2 423 431 |
institution |
Open Polar |
collection |
University of Liège: ORBi (Open Repository and Bibliography) |
op_collection_id |
ftorbi |
language |
English |
topic |
16S Arctic Regions Bacterial Proteins Bacterial Proteins: classification Bacterial Proteins: genetics Bacterial Proteins: metabolism Chromatography Cyanobacteria Cyanobacteria: genetics Cyanobacteria: isolation purification Cyanobacteria: metabolism Ecosystem Enzyme-Linked Immunosorbent Assay Fresh Water Fresh Water: chemistry Fresh Water: microbiology High Pressure Liquid Mass Spectrometry Messenger Messenger: metabolism Microcystins Microcystins: analysis Phylogeny RNA Ribosomal Saxitoxin Saxitoxin: analysis Life sciences Microbiology Sciences du vivant Microbiologie |
spellingShingle |
16S Arctic Regions Bacterial Proteins Bacterial Proteins: classification Bacterial Proteins: genetics Bacterial Proteins: metabolism Chromatography Cyanobacteria Cyanobacteria: genetics Cyanobacteria: isolation purification Cyanobacteria: metabolism Ecosystem Enzyme-Linked Immunosorbent Assay Fresh Water Fresh Water: chemistry Fresh Water: microbiology High Pressure Liquid Mass Spectrometry Messenger Messenger: metabolism Microcystins Microcystins: analysis Phylogeny RNA Ribosomal Saxitoxin Saxitoxin: analysis Life sciences Microbiology Sciences du vivant Microbiologie Kleinteich, Julia Wood, Susanna A Puddick, Jonathan Schleheck, David Küpper, Frithjof C. Dietrich, Daniel Potent toxins in Arctic environments--presence of saxitoxins and an unusual microcystin variant in Arctic freshwater ecosystems. |
topic_facet |
16S Arctic Regions Bacterial Proteins Bacterial Proteins: classification Bacterial Proteins: genetics Bacterial Proteins: metabolism Chromatography Cyanobacteria Cyanobacteria: genetics Cyanobacteria: isolation purification Cyanobacteria: metabolism Ecosystem Enzyme-Linked Immunosorbent Assay Fresh Water Fresh Water: chemistry Fresh Water: microbiology High Pressure Liquid Mass Spectrometry Messenger Messenger: metabolism Microcystins Microcystins: analysis Phylogeny RNA Ribosomal Saxitoxin Saxitoxin: analysis Life sciences Microbiology Sciences du vivant Microbiologie |
description |
peer reviewed Cyanobacteria are the predominant phototrophs in freshwater ecosystems of the polar regions where they commonly form extensive benthic mats. Despite their major biological role in these ecosystems, little attention has been paid to their physiology and biochemistry. An important feature of cyanobacteria from the temperate and tropical regions is the production of a large variety of toxic secondary metabolites. In Antarctica, and more recently in the Arctic, the cyanobacterial toxins microcystin and nodularin (Antarctic only) have been detected in freshwater microbial mats. To date other cyanobacterial toxins have not been reported from these locations. Five Arctic cyanobacterial communities were screened for saxitoxin, another common cyanobacterial toxin, and microcystins using immunological, spectroscopic and molecular methods. Saxitoxin was detected for the first time in cyanobacteria from the Arctic. In addition, an unusual microcystin variant was identified using liquid chromatography-mass spectrometry. Gene expression analyses confirmed the analytical findings, whereby parts of the sxt and mcy operon involved in saxitoxin and microcystin synthesis, were detected and sequenced in one and five of the Arctic cyanobacterial samples, respectively. The detection of these compounds in the cryosphere improves the understanding of the biogeography and distribution of toxic cyanobacteria globally. The sequences of sxt and mcy genes provided from this habitat for the first time may help to clarify the evolutionary origin of toxin production in cyanobacteria. |
format |
Article in Journal/Newspaper |
author |
Kleinteich, Julia Wood, Susanna A Puddick, Jonathan Schleheck, David Küpper, Frithjof C. Dietrich, Daniel |
author_facet |
Kleinteich, Julia Wood, Susanna A Puddick, Jonathan Schleheck, David Küpper, Frithjof C. Dietrich, Daniel |
author_sort |
Kleinteich, Julia |
title |
Potent toxins in Arctic environments--presence of saxitoxins and an unusual microcystin variant in Arctic freshwater ecosystems. |
title_short |
Potent toxins in Arctic environments--presence of saxitoxins and an unusual microcystin variant in Arctic freshwater ecosystems. |
title_full |
Potent toxins in Arctic environments--presence of saxitoxins and an unusual microcystin variant in Arctic freshwater ecosystems. |
title_fullStr |
Potent toxins in Arctic environments--presence of saxitoxins and an unusual microcystin variant in Arctic freshwater ecosystems. |
title_full_unstemmed |
Potent toxins in Arctic environments--presence of saxitoxins and an unusual microcystin variant in Arctic freshwater ecosystems. |
title_sort |
potent toxins in arctic environments--presence of saxitoxins and an unusual microcystin variant in arctic freshwater ecosystems. |
publisher |
Elsevier Ireland Ltd |
publishDate |
2013 |
url |
https://orbi.uliege.be/handle/2268/178154 https://doi.org/10.1016/j.cbi.2013.04.011 |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Chemico-Biological Interactions, 206 (2), 423-31 (2013) |
op_relation |
http://www.ncbi.nlm.nih.gov/pubmed/23648386 urn:issn:0009-2797 urn:issn:1872-7786 https://orbi.uliege.be/handle/2268/178154 info:hdl:2268/178154 doi:10.1016/j.cbi.2013.04.011 scopus-id:2-s2.0-84888291241 info:pmid:23648386 |
op_rights |
restricted access http://purl.org/coar/access_right/c_16ec info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.cbi.2013.04.011 |
container_title |
Chemico-Biological Interactions |
container_volume |
206 |
container_issue |
2 |
container_start_page |
423 |
op_container_end_page |
431 |
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1796946811654504448 |