Crystallization and preliminary X-ray analysis of a bacterial psychrophilic enzyme, phosphoglycerate kinase
peer reviewed The glycolytic enzyme phosphoglycerate kinase (PGK) from the Antarctic microorganism Pseudomonas sp. TACII18 is a cold-adapted enzyme that displays a high specific activity at low temperatures and decreased thermostability relative to its mesophilic counterpart. Herein, the preliminary...
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Blackwell Publishing
2001
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ftorbi:oai:orbi.ulg.ac.be:2268/16748 2024-04-21T07:51:55+00:00 Crystallization and preliminary X-ray analysis of a bacterial psychrophilic enzyme, phosphoglycerate kinase Mandelman, D. Bentahir, M. Feller, Georges Gerday, Colette Haser, R. 2001 https://orbi.uliege.be/handle/2268/16748 https://doi.org/10.1107/S0907444901012069 en eng Blackwell Publishing urn:issn:0907-4449 urn:issn:1399-0047 https://orbi.uliege.be/handle/2268/16748 info:hdl:2268/16748 doi:10.1107/S0907444901012069 scopus-id:2-s2.0-0034767605 info:pmid:11679738 restricted access http://purl.org/coar/access_right/c_16ec info:eu-repo/semantics/restrictedAccess Acta Crystallographica. Section D, Biological Crystallography, 57 (Pt 11), 1666-8 (2001) Crystallization Crystallography X-Ray Phosphoglycerate Kinase/*chemistry Protein Conformation Pseudomonas/*enzymology Support Non-U.S. Gov't Life sciences Biochemistry biophysics & molecular biology Sciences du vivant Biochimie biophysique & biologie moléculaire journal article http://purl.org/coar/resource_type/c_6501 info:eu-repo/semantics/article peer reviewed 2001 ftorbi https://doi.org/10.1107/S0907444901012069 2024-03-27T14:55:01Z peer reviewed The glycolytic enzyme phosphoglycerate kinase (PGK) from the Antarctic microorganism Pseudomonas sp. TACII18 is a cold-adapted enzyme that displays a high specific activity at low temperatures and decreased thermostability relative to its mesophilic counterpart. Herein, the preliminary crystallization and structure solution of psychrophilic PGK in its native form and cocrystallized with 3-phosphoglyceric acid (3-PGA) and the ATP analogue adenylyl imidophosphate (AMP-PNP) is reported. The complexed form of PGK crystallized in 2-3 d at 290 K, whereas the native form of the enzyme required 8-12 months. Morphologically, both crystal forms are similar and X-ray diffraction experiments indicate that the crystals are isomorphous. The crystals diffracted to a resolution of 2.0 A and belong to the space group P3(2). with unit-cell parameters a = b = 58.5, c = 85.4 A. Article in Journal/Newspaper Antarc* Antarctic University of Liège: ORBi (Open Repository and Bibliography) Acta Crystallographica Section D Biological Crystallography 57 11 1666 1668 |
institution |
Open Polar |
collection |
University of Liège: ORBi (Open Repository and Bibliography) |
op_collection_id |
ftorbi |
language |
English |
topic |
Crystallization Crystallography X-Ray Phosphoglycerate Kinase/*chemistry Protein Conformation Pseudomonas/*enzymology Support Non-U.S. Gov't Life sciences Biochemistry biophysics & molecular biology Sciences du vivant Biochimie biophysique & biologie moléculaire |
spellingShingle |
Crystallization Crystallography X-Ray Phosphoglycerate Kinase/*chemistry Protein Conformation Pseudomonas/*enzymology Support Non-U.S. Gov't Life sciences Biochemistry biophysics & molecular biology Sciences du vivant Biochimie biophysique & biologie moléculaire Mandelman, D. Bentahir, M. Feller, Georges Gerday, Colette Haser, R. Crystallization and preliminary X-ray analysis of a bacterial psychrophilic enzyme, phosphoglycerate kinase |
topic_facet |
Crystallization Crystallography X-Ray Phosphoglycerate Kinase/*chemistry Protein Conformation Pseudomonas/*enzymology Support Non-U.S. Gov't Life sciences Biochemistry biophysics & molecular biology Sciences du vivant Biochimie biophysique & biologie moléculaire |
description |
peer reviewed The glycolytic enzyme phosphoglycerate kinase (PGK) from the Antarctic microorganism Pseudomonas sp. TACII18 is a cold-adapted enzyme that displays a high specific activity at low temperatures and decreased thermostability relative to its mesophilic counterpart. Herein, the preliminary crystallization and structure solution of psychrophilic PGK in its native form and cocrystallized with 3-phosphoglyceric acid (3-PGA) and the ATP analogue adenylyl imidophosphate (AMP-PNP) is reported. The complexed form of PGK crystallized in 2-3 d at 290 K, whereas the native form of the enzyme required 8-12 months. Morphologically, both crystal forms are similar and X-ray diffraction experiments indicate that the crystals are isomorphous. The crystals diffracted to a resolution of 2.0 A and belong to the space group P3(2). with unit-cell parameters a = b = 58.5, c = 85.4 A. |
format |
Article in Journal/Newspaper |
author |
Mandelman, D. Bentahir, M. Feller, Georges Gerday, Colette Haser, R. |
author_facet |
Mandelman, D. Bentahir, M. Feller, Georges Gerday, Colette Haser, R. |
author_sort |
Mandelman, D. |
title |
Crystallization and preliminary X-ray analysis of a bacterial psychrophilic enzyme, phosphoglycerate kinase |
title_short |
Crystallization and preliminary X-ray analysis of a bacterial psychrophilic enzyme, phosphoglycerate kinase |
title_full |
Crystallization and preliminary X-ray analysis of a bacterial psychrophilic enzyme, phosphoglycerate kinase |
title_fullStr |
Crystallization and preliminary X-ray analysis of a bacterial psychrophilic enzyme, phosphoglycerate kinase |
title_full_unstemmed |
Crystallization and preliminary X-ray analysis of a bacterial psychrophilic enzyme, phosphoglycerate kinase |
title_sort |
crystallization and preliminary x-ray analysis of a bacterial psychrophilic enzyme, phosphoglycerate kinase |
publisher |
Blackwell Publishing |
publishDate |
2001 |
url |
https://orbi.uliege.be/handle/2268/16748 https://doi.org/10.1107/S0907444901012069 |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Acta Crystallographica. Section D, Biological Crystallography, 57 (Pt 11), 1666-8 (2001) |
op_relation |
urn:issn:0907-4449 urn:issn:1399-0047 https://orbi.uliege.be/handle/2268/16748 info:hdl:2268/16748 doi:10.1107/S0907444901012069 scopus-id:2-s2.0-0034767605 info:pmid:11679738 |
op_rights |
restricted access http://purl.org/coar/access_right/c_16ec info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1107/S0907444901012069 |
container_title |
Acta Crystallographica Section D Biological Crystallography |
container_volume |
57 |
container_issue |
11 |
container_start_page |
1666 |
op_container_end_page |
1668 |
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1796935208152334336 |