Nucleotide Sequence of the Lipase Gene Lip2 from the Antarctic Psychrotroph Moraxella Ta144 and Site-Specific Mutagenesis of the Conserved Serine and Histidine Residues
peer reviewed The lip2 gene from the antarctic psychotroph Moraxella TA144 was sequenced. The primary structure of the Lip2 preprotein deduced from the nucleotide sequence is composed of 433 amino acids with a predicted Mr of 47,222. This enzyme contains a Ser-centered consensus sequence and a conse...
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Mary Ann Liebert
1991
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Online Access: | https://orbi.uliege.be/handle/2268/16462 https://orbi.uliege.be/bitstream/2268/16462/1/DNACellBiol_91_lip2.pdf https://doi.org/10.1089/dna.1991.10.381 |
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ftorbi:oai:orbi.ulg.ac.be:2268/16462 2024-04-21T07:52:39+00:00 Nucleotide Sequence of the Lipase Gene Lip2 from the Antarctic Psychrotroph Moraxella Ta144 and Site-Specific Mutagenesis of the Conserved Serine and Histidine Residues Feller, Georges Thiry, M. Gerday, Charles 1991-06 https://orbi.uliege.be/handle/2268/16462 https://orbi.uliege.be/bitstream/2268/16462/1/DNACellBiol_91_lip2.pdf https://doi.org/10.1089/dna.1991.10.381 en eng Mary Ann Liebert urn:issn:1044-5498 urn:issn:1557-7430 https://orbi.uliege.be/handle/2268/16462 info:hdl:2268/16462 https://orbi.uliege.be/bitstream/2268/16462/1/DNACellBiol_91_lip2.pdf doi:10.1089/dna.1991.10.381 scopus-id:2-s2.0-0025732362 info:pmid:1907455 open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess DNA and Cell Biology, 10 (5), 381-8 (1991-06) Life sciences Biochemistry biophysics & molecular biology Sciences du vivant Biochimie biophysique & biologie moléculaire journal article http://purl.org/coar/resource_type/c_6501 info:eu-repo/semantics/article peer reviewed 1991 ftorbi https://doi.org/10.1089/dna.1991.10.381 2024-03-27T14:52:24Z peer reviewed The lip2 gene from the antarctic psychotroph Moraxella TA144 was sequenced. The primary structure of the Lip2 preprotein deduced from the nucleotide sequence is composed of 433 amino acids with a predicted Mr of 47,222. This enzyme contains a Ser-centered consensus sequence and a conserved His-Gly dipeptide found in most lipase amino-terminal domains. These sequences are involved in the lipase active site conformation since substitution of the conserved Ser or His residues by Ala and Gln, respectively, results in the loss of both lipase and esterase activities. Structural factors that would allow proper enzyme flexibility at low temperatures are discussed. It is suggested that only subtle changes in the primary structure of these psychrotrophic enzymes can account for their ability to catalyze lipolysis at temperatures close to 0 degrees C. Article in Journal/Newspaper Antarc* Antarctic University of Liège: ORBi (Open Repository and Bibliography) DNA and Cell Biology 10 5 381 388 |
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Open Polar |
collection |
University of Liège: ORBi (Open Repository and Bibliography) |
op_collection_id |
ftorbi |
language |
English |
topic |
Life sciences Biochemistry biophysics & molecular biology Sciences du vivant Biochimie biophysique & biologie moléculaire |
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Life sciences Biochemistry biophysics & molecular biology Sciences du vivant Biochimie biophysique & biologie moléculaire Feller, Georges Thiry, M. Gerday, Charles Nucleotide Sequence of the Lipase Gene Lip2 from the Antarctic Psychrotroph Moraxella Ta144 and Site-Specific Mutagenesis of the Conserved Serine and Histidine Residues |
topic_facet |
Life sciences Biochemistry biophysics & molecular biology Sciences du vivant Biochimie biophysique & biologie moléculaire |
description |
peer reviewed The lip2 gene from the antarctic psychotroph Moraxella TA144 was sequenced. The primary structure of the Lip2 preprotein deduced from the nucleotide sequence is composed of 433 amino acids with a predicted Mr of 47,222. This enzyme contains a Ser-centered consensus sequence and a conserved His-Gly dipeptide found in most lipase amino-terminal domains. These sequences are involved in the lipase active site conformation since substitution of the conserved Ser or His residues by Ala and Gln, respectively, results in the loss of both lipase and esterase activities. Structural factors that would allow proper enzyme flexibility at low temperatures are discussed. It is suggested that only subtle changes in the primary structure of these psychrotrophic enzymes can account for their ability to catalyze lipolysis at temperatures close to 0 degrees C. |
format |
Article in Journal/Newspaper |
author |
Feller, Georges Thiry, M. Gerday, Charles |
author_facet |
Feller, Georges Thiry, M. Gerday, Charles |
author_sort |
Feller, Georges |
title |
Nucleotide Sequence of the Lipase Gene Lip2 from the Antarctic Psychrotroph Moraxella Ta144 and Site-Specific Mutagenesis of the Conserved Serine and Histidine Residues |
title_short |
Nucleotide Sequence of the Lipase Gene Lip2 from the Antarctic Psychrotroph Moraxella Ta144 and Site-Specific Mutagenesis of the Conserved Serine and Histidine Residues |
title_full |
Nucleotide Sequence of the Lipase Gene Lip2 from the Antarctic Psychrotroph Moraxella Ta144 and Site-Specific Mutagenesis of the Conserved Serine and Histidine Residues |
title_fullStr |
Nucleotide Sequence of the Lipase Gene Lip2 from the Antarctic Psychrotroph Moraxella Ta144 and Site-Specific Mutagenesis of the Conserved Serine and Histidine Residues |
title_full_unstemmed |
Nucleotide Sequence of the Lipase Gene Lip2 from the Antarctic Psychrotroph Moraxella Ta144 and Site-Specific Mutagenesis of the Conserved Serine and Histidine Residues |
title_sort |
nucleotide sequence of the lipase gene lip2 from the antarctic psychrotroph moraxella ta144 and site-specific mutagenesis of the conserved serine and histidine residues |
publisher |
Mary Ann Liebert |
publishDate |
1991 |
url |
https://orbi.uliege.be/handle/2268/16462 https://orbi.uliege.be/bitstream/2268/16462/1/DNACellBiol_91_lip2.pdf https://doi.org/10.1089/dna.1991.10.381 |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
DNA and Cell Biology, 10 (5), 381-8 (1991-06) |
op_relation |
urn:issn:1044-5498 urn:issn:1557-7430 https://orbi.uliege.be/handle/2268/16462 info:hdl:2268/16462 https://orbi.uliege.be/bitstream/2268/16462/1/DNACellBiol_91_lip2.pdf doi:10.1089/dna.1991.10.381 scopus-id:2-s2.0-0025732362 info:pmid:1907455 |
op_rights |
open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1089/dna.1991.10.381 |
container_title |
DNA and Cell Biology |
container_volume |
10 |
container_issue |
5 |
container_start_page |
381 |
op_container_end_page |
388 |
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1796935846722535424 |