Cloning and Expression in Escherichia Coli of Three Lipase-Encoding Genes from the Psychrotrophic Antarctic Strain Moraxella Ta144
peer reviewed The cloning and expression of genes from a psychrotrophic bacterium in a mesophilic host are described. Three lipase (Lip)-encoding genes (lip) from the antarctic psychrotroph, Moraxella TA144, were cloned by inserting Sau3AI-generated DNA fragments into the BamHI site of the pSP73 pla...
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1991
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Online Access: | https://orbi.uliege.be/handle/2268/16460 https://doi.org/10.1016/0378-1119(91)90548-P |
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ftorbi:oai:orbi.ulg.ac.be:2268/16460 2024-04-21T07:47:39+00:00 Cloning and Expression in Escherichia Coli of Three Lipase-Encoding Genes from the Psychrotrophic Antarctic Strain Moraxella Ta144 Feller, Georges Thiry, M. Arpigny, J. L. Gerday, Charles 1991-06-15 https://orbi.uliege.be/handle/2268/16460 https://doi.org/10.1016/0378-1119(91)90548-P en eng Elsevier urn:issn:0378-1119 urn:issn:1879-0038 https://orbi.uliege.be/handle/2268/16460 info:hdl:2268/16460 doi:10.1016/0378-1119(91)90548-P scopus-id:2-s2.0-0025773328 info:pmid:1864500 restricted access http://purl.org/coar/access_right/c_16ec info:eu-repo/semantics/restrictedAccess Gene, 102 (1), 111-5 (1991-06-15) Life sciences Biochemistry biophysics & molecular biology Sciences du vivant Biochimie biophysique & biologie moléculaire journal article http://purl.org/coar/resource_type/c_6501 info:eu-repo/semantics/article peer reviewed 1991 ftorbi https://doi.org/10.1016/0378-1119(91)90548-P 2024-03-27T14:55:01Z peer reviewed The cloning and expression of genes from a psychrotrophic bacterium in a mesophilic host are described. Three lipase (Lip)-encoding genes (lip) from the antarctic psychrotroph, Moraxella TA144, were cloned by inserting Sau3AI-generated DNA fragments into the BamHI site of the pSP73 plasmid vector. To prevent heat denaturation of the gene product, the screening procedure on agar plates containing an emulsified lipid involved growing of Escherichia coli recombinant colonies at 25 degrees C followed by incubation at 0 degree C. The three recombinant (reLip) were cell-associated and differed by their respective specificity towards p-nitrophenyl esters of various aliphatic chain lengths. These cloned reLip conserved the main character of the wild-type enzymes, i.e. a dramatic shift of the optimal temperature of activity towards low temperatures and pronounced heat lability. Article in Journal/Newspaper Antarc* Antarctic University of Liège: ORBi (Open Repository and Bibliography) Gene 102 1 111 115 |
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Open Polar |
collection |
University of Liège: ORBi (Open Repository and Bibliography) |
op_collection_id |
ftorbi |
language |
English |
topic |
Life sciences Biochemistry biophysics & molecular biology Sciences du vivant Biochimie biophysique & biologie moléculaire |
spellingShingle |
Life sciences Biochemistry biophysics & molecular biology Sciences du vivant Biochimie biophysique & biologie moléculaire Feller, Georges Thiry, M. Arpigny, J. L. Gerday, Charles Cloning and Expression in Escherichia Coli of Three Lipase-Encoding Genes from the Psychrotrophic Antarctic Strain Moraxella Ta144 |
topic_facet |
Life sciences Biochemistry biophysics & molecular biology Sciences du vivant Biochimie biophysique & biologie moléculaire |
description |
peer reviewed The cloning and expression of genes from a psychrotrophic bacterium in a mesophilic host are described. Three lipase (Lip)-encoding genes (lip) from the antarctic psychrotroph, Moraxella TA144, were cloned by inserting Sau3AI-generated DNA fragments into the BamHI site of the pSP73 plasmid vector. To prevent heat denaturation of the gene product, the screening procedure on agar plates containing an emulsified lipid involved growing of Escherichia coli recombinant colonies at 25 degrees C followed by incubation at 0 degree C. The three recombinant (reLip) were cell-associated and differed by their respective specificity towards p-nitrophenyl esters of various aliphatic chain lengths. These cloned reLip conserved the main character of the wild-type enzymes, i.e. a dramatic shift of the optimal temperature of activity towards low temperatures and pronounced heat lability. |
format |
Article in Journal/Newspaper |
author |
Feller, Georges Thiry, M. Arpigny, J. L. Gerday, Charles |
author_facet |
Feller, Georges Thiry, M. Arpigny, J. L. Gerday, Charles |
author_sort |
Feller, Georges |
title |
Cloning and Expression in Escherichia Coli of Three Lipase-Encoding Genes from the Psychrotrophic Antarctic Strain Moraxella Ta144 |
title_short |
Cloning and Expression in Escherichia Coli of Three Lipase-Encoding Genes from the Psychrotrophic Antarctic Strain Moraxella Ta144 |
title_full |
Cloning and Expression in Escherichia Coli of Three Lipase-Encoding Genes from the Psychrotrophic Antarctic Strain Moraxella Ta144 |
title_fullStr |
Cloning and Expression in Escherichia Coli of Three Lipase-Encoding Genes from the Psychrotrophic Antarctic Strain Moraxella Ta144 |
title_full_unstemmed |
Cloning and Expression in Escherichia Coli of Three Lipase-Encoding Genes from the Psychrotrophic Antarctic Strain Moraxella Ta144 |
title_sort |
cloning and expression in escherichia coli of three lipase-encoding genes from the psychrotrophic antarctic strain moraxella ta144 |
publisher |
Elsevier |
publishDate |
1991 |
url |
https://orbi.uliege.be/handle/2268/16460 https://doi.org/10.1016/0378-1119(91)90548-P |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Gene, 102 (1), 111-5 (1991-06-15) |
op_relation |
urn:issn:0378-1119 urn:issn:1879-0038 https://orbi.uliege.be/handle/2268/16460 info:hdl:2268/16460 doi:10.1016/0378-1119(91)90548-P scopus-id:2-s2.0-0025773328 info:pmid:1864500 |
op_rights |
restricted access http://purl.org/coar/access_right/c_16ec info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/0378-1119(91)90548-P |
container_title |
Gene |
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102 |
container_issue |
1 |
container_start_page |
111 |
op_container_end_page |
115 |
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1796946877701160960 |