Cloning, Sequence and Structural Features of a Lipase from the Antarctic Facultative Psychrophile Psychrobacter Immobilis B10

• Published in: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression
• Main Authors: Arpigny, J. L., Feller, Georges, Gerday, Charles
• Format: Article in Journal/Newspaper
• Language: English
• Published: Elsevier 1993
• Subjects: Life sciences; Biochemistry; biophysics & molecular biology; Sciences du vivant; Biochimie; biophysique & biologie moléculaire; Antarc*; Antarctic
• Online Access: https://orbi.uliege.be/handle/2268/16332; https://doi.org/10.1016/0167-4781(93)90078-R

peer reviewed A lipase gene (lip1) from the facultative psychrophilic strain Psychrobacter immobilis B10 has been cloned and sequenced. The deduced preprotein sequence is composed of 317 amino acids with a predicted M(r) of 35,288. A primary structure alignment of lipases including lip1 shows conserved elements for which a structural role is proposed in the light of recent crystallographic studies. The analysis of the psychrophilic enzyme sequence suggests characteristics in relation with the adaptation to cold.