The structure of a cold-adapted family 8 xylanase at 1.3 angstrom resolution - Structural adaptations to cold and investigation of the active site
peer reviewed Enzymes from psychrophilic organisms differ from their mesophilic counterparts in having a lower thermo-stability and a higher specific activity at low and moderate temperatures. The current consensus is that they have an increased flexibility, enhancing accommodation and transformatio...
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2003
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ftorbi:oai:orbi.ulg.ac.be:2268/15844 2024-04-21T07:51:26+00:00 The structure of a cold-adapted family 8 xylanase at 1.3 angstrom resolution - Structural adaptations to cold and investigation of the active site Van Petegem, F. Collins, T. Meuwis, Marie-Alice Gerday, Charles Feller, Georges Van Beeumen, J. 2003-02-28 https://orbi.uliege.be/handle/2268/15844 https://orbi.uliege.be/bitstream/2268/15844/1/JBC_2003_Xyl.pdf https://doi.org/10.1074/jbc.M206862200 en eng Amer Soc Biochemistry Molecular Biology Inc urn:issn:0021-9258 urn:issn:1083-351X https://orbi.uliege.be/handle/2268/15844 info:hdl:2268/15844 https://orbi.uliege.be/bitstream/2268/15844/1/JBC_2003_Xyl.pdf doi:10.1074/jbc.M206862200 scopus-id:2-s2.0-0037470085 info:pmid:12475991 open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess Journal of Biological Chemistry, 278 (9), 7531-7539 (2003-02-28) Life sciences Biochemistry biophysics & molecular biology Sciences du vivant Biochimie biophysique & biologie moléculaire journal article http://purl.org/coar/resource_type/c_6501 info:eu-repo/semantics/article peer reviewed 2003 ftorbi https://doi.org/10.1074/jbc.M206862200 2024-03-27T14:52:24Z peer reviewed Enzymes from psychrophilic organisms differ from their mesophilic counterparts in having a lower thermo-stability and a higher specific activity at low and moderate temperatures. The current consensus is that they have an increased flexibility, enhancing accommodation and transformation of the substrates at low energy costs. Here we describe the structure of the xylanase from the Antarctic bacterium Pseudoalteromonas haloplanktis at 1.3 Angstrom resolution. Xylanases are usually grouped into glycosyl hydrolase families 10 and 11, but this enzyme belongs to family 8. The fold differs from that of other known xylanases and can be described as an (alpha/alpha)(6) barrel. Various parameters that may explain the cold-adapted properties were examined and indicated that the protein has a reduced number of salt bridges and an increased exposure of hydrophobic residues. The crystal structures of a complex with xylobiose and of mutant D144N were obtained at 1.2 and 1.5 A resolution, respectively. Analysis of the various substrate binding sites shows that the +3 and -3 subsites are rearranged as compared to those of a family 8 homolog, while the xylobiose complex suggests the existence of a +4 subsite. A decreased acidity of the substrate binding cleft and an increased flexibility of aromatic residues lining the subsites may enhance the rate at which substrate is bound. Article in Journal/Newspaper Antarc* Antarctic University of Liège: ORBi (Open Repository and Bibliography) Journal of Biological Chemistry 278 9 7531 7539 |
institution |
Open Polar |
collection |
University of Liège: ORBi (Open Repository and Bibliography) |
op_collection_id |
ftorbi |
language |
English |
topic |
Life sciences Biochemistry biophysics & molecular biology Sciences du vivant Biochimie biophysique & biologie moléculaire |
spellingShingle |
Life sciences Biochemistry biophysics & molecular biology Sciences du vivant Biochimie biophysique & biologie moléculaire Van Petegem, F. Collins, T. Meuwis, Marie-Alice Gerday, Charles Feller, Georges Van Beeumen, J. The structure of a cold-adapted family 8 xylanase at 1.3 angstrom resolution - Structural adaptations to cold and investigation of the active site |
topic_facet |
Life sciences Biochemistry biophysics & molecular biology Sciences du vivant Biochimie biophysique & biologie moléculaire |
description |
peer reviewed Enzymes from psychrophilic organisms differ from their mesophilic counterparts in having a lower thermo-stability and a higher specific activity at low and moderate temperatures. The current consensus is that they have an increased flexibility, enhancing accommodation and transformation of the substrates at low energy costs. Here we describe the structure of the xylanase from the Antarctic bacterium Pseudoalteromonas haloplanktis at 1.3 Angstrom resolution. Xylanases are usually grouped into glycosyl hydrolase families 10 and 11, but this enzyme belongs to family 8. The fold differs from that of other known xylanases and can be described as an (alpha/alpha)(6) barrel. Various parameters that may explain the cold-adapted properties were examined and indicated that the protein has a reduced number of salt bridges and an increased exposure of hydrophobic residues. The crystal structures of a complex with xylobiose and of mutant D144N were obtained at 1.2 and 1.5 A resolution, respectively. Analysis of the various substrate binding sites shows that the +3 and -3 subsites are rearranged as compared to those of a family 8 homolog, while the xylobiose complex suggests the existence of a +4 subsite. A decreased acidity of the substrate binding cleft and an increased flexibility of aromatic residues lining the subsites may enhance the rate at which substrate is bound. |
format |
Article in Journal/Newspaper |
author |
Van Petegem, F. Collins, T. Meuwis, Marie-Alice Gerday, Charles Feller, Georges Van Beeumen, J. |
author_facet |
Van Petegem, F. Collins, T. Meuwis, Marie-Alice Gerday, Charles Feller, Georges Van Beeumen, J. |
author_sort |
Van Petegem, F. |
title |
The structure of a cold-adapted family 8 xylanase at 1.3 angstrom resolution - Structural adaptations to cold and investigation of the active site |
title_short |
The structure of a cold-adapted family 8 xylanase at 1.3 angstrom resolution - Structural adaptations to cold and investigation of the active site |
title_full |
The structure of a cold-adapted family 8 xylanase at 1.3 angstrom resolution - Structural adaptations to cold and investigation of the active site |
title_fullStr |
The structure of a cold-adapted family 8 xylanase at 1.3 angstrom resolution - Structural adaptations to cold and investigation of the active site |
title_full_unstemmed |
The structure of a cold-adapted family 8 xylanase at 1.3 angstrom resolution - Structural adaptations to cold and investigation of the active site |
title_sort |
structure of a cold-adapted family 8 xylanase at 1.3 angstrom resolution - structural adaptations to cold and investigation of the active site |
publisher |
Amer Soc Biochemistry Molecular Biology Inc |
publishDate |
2003 |
url |
https://orbi.uliege.be/handle/2268/15844 https://orbi.uliege.be/bitstream/2268/15844/1/JBC_2003_Xyl.pdf https://doi.org/10.1074/jbc.M206862200 |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Journal of Biological Chemistry, 278 (9), 7531-7539 (2003-02-28) |
op_relation |
urn:issn:0021-9258 urn:issn:1083-351X https://orbi.uliege.be/handle/2268/15844 info:hdl:2268/15844 https://orbi.uliege.be/bitstream/2268/15844/1/JBC_2003_Xyl.pdf doi:10.1074/jbc.M206862200 scopus-id:2-s2.0-0037470085 info:pmid:12475991 |
op_rights |
open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1074/jbc.M206862200 |
container_title |
Journal of Biological Chemistry |
container_volume |
278 |
container_issue |
9 |
container_start_page |
7531 |
op_container_end_page |
7539 |
_version_ |
1796934790114443264 |