Use of response surface methodology for the optimization of the lipase-catalyzed synthesis of mannosyl myristate in pure ionic liquid

• Published in: Process Biochemistry
• Main Authors: Galonde, Nadine, Brostaux, Yves, Richard, Gaetan, Nott, Katherine, Jérôme, Christine, Fauconnier, Marie-Laure
• Format: Article in Journal/Newspaper
• Language: English
• Published: Elsevier Science 2013
• Subjects: Sugar esters; Ionic liquids; Novozym®435; RSM plots; Full factorial design; Transesterification; Life sciences; Biotechnology; Sciences du vivant; Biotechnologie; Antarc*; Antarctica
• Online Access: https://orbi.uliege.be/handle/2268/156070; https://doi.org/10.1016/j.procbio.2013.08.023

peer reviewed The purpose of the present study is to find the conditions allowing to reach the highest 24 h-yield(24 h- ) for the synthesis of mannosyl myristate catalyzed by the immobilized lipase B from Candida antarctica (Novozym®435) in the ionic liquid (IL) [Bmpyrr][TFO] (1-butyl-1-methylpyrrolidinium tri-fluoromethanesulfonate). A full factorial design (FFD) was used in order to study the influence of three variables (temperature, mannose/vinyl myristate ratio and total substrate quantity) on the 24 h- . This design led to a model based on a second order polynomial response function. The resulting predicted con-tour plots have shown that the highest 24 h- should be obtained with high temperatures, low sugar/vinylester molar ratio and intermediate total substrate quantities (mmol). The model has been successfully verified and experimentally confirmed at the optimal conditions of 80◦C, substrate molar ratio of 1/10and total substrate quantity of 0.26 mmol leading to the highest predicted 24 h- of 72.2%.