The diversity and tolerance to osmotic stress of East Antarctic filamentous Cyanobacteria
Filamentous cyanobacteria are keystone species in Antarctic lake ecosystems; they are the basis of the simple foodwebs, play a crucial role in biogeochemical cycling and form the structure of benthic microbial mats which act as habitats for other prokaryotic and (micro-eukaryotic biota. Despite this...
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ftorbi:oai:orbi.ulg.ac.be:2268/153857 2024-04-21T07:47:20+00:00 The diversity and tolerance to osmotic stress of East Antarctic filamentous Cyanobacteria Obbels, Dagmar Verleyen, Elie Tytgat, Bjorn Elster, Josef Strunecki, Otakar Wilmotte, Annick Willems, Anne Sabbe, Koen Vyverman, Wim CIP - Centre d'Ingénierie des Protéines - ULiège 2013-07 Poster A0 https://orbi.uliege.be/handle/2268/153857 en eng https://orbi.uliege.be/handle/2268/153857 info:hdl:2268/153857 11th SCAR Biology Symposium, Barcelona, Spain [ES], 15-19 juillet 2013 Antarctic Cyanobacteria Life sciences Microbiology Sciences du vivant Microbiologie conference poster not in proceedings http://purl.org/coar/resource_type/c_18co info:eu-repo/semantics/conferencePoster 2013 ftorbi 2024-03-27T14:58:35Z Filamentous cyanobacteria are keystone species in Antarctic lake ecosystems; they are the basis of the simple foodwebs, play a crucial role in biogeochemical cycling and form the structure of benthic microbial mats which act as habitats for other prokaryotic and (micro-eukaryotic biota. Despite this, little is known about their diversity, adaptation and survival strategies in the extreme Antarctic conditions. We studied the uncultivated prokaryotic diversity using a 454 metagenomic analysis at the 16S rRNA level (V1-V3 region) in Continental Antarctic lakes situated along a conductivity gradient (0.014-142.02 mS/cm). The quality and length of the amplicons was analyzed with a custom-made Mothur pipeline and the resulting sequences were mapped against the Greengenes database, which includes CyanoDB. Almost 27% of the sequences could be assigned to the phylum of the cyanobacteria. The most abundant cyanobacteria in the dataset belonged to the genera Microcoleus, Leptolyngbya, Pseudanabaena, Nodularia and Phormidum. Some 16S rRNA types (at the 97% similarity level), such as sequences related to Leptolynbya antarctica, were present in both freshwater and hypersaline lakes. In order to further investigate this distribution, we isolated filaments of Leptolyngbya from seven lakes with conductivities ranging between 26.8 mS/cm and 0.038 mS/cm. The complete 16S rRNA and ITS genes of the isolates were subsequently sequenced. We found several 16S types related to different lineages of filamentous cyanobacteria in the seven lakes that were supported by ITS data. Two 16S types, belonging to a Leptolyngbya antarctica and Leptolyngbya sp., were each present in two different freshwater lakes. Two different 16S types, both belonging to Leptolynbya antarctica were present in a freshwater and hypersaline lake, which indicates a high ‘intraspecific’ molecular diversity. In order to better understand the adaptation and/or wide tolerance to osmotic stress, we are currently performing ecophysiological experiments with these isolates ... Conference Object Antarc* Antarctic Antarctica University of Liège: ORBi (Open Repository and Bibliography) |
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Open Polar |
collection |
University of Liège: ORBi (Open Repository and Bibliography) |
op_collection_id |
ftorbi |
language |
English |
topic |
Antarctic Cyanobacteria Life sciences Microbiology Sciences du vivant Microbiologie |
spellingShingle |
Antarctic Cyanobacteria Life sciences Microbiology Sciences du vivant Microbiologie Obbels, Dagmar Verleyen, Elie Tytgat, Bjorn Elster, Josef Strunecki, Otakar Wilmotte, Annick Willems, Anne Sabbe, Koen Vyverman, Wim The diversity and tolerance to osmotic stress of East Antarctic filamentous Cyanobacteria |
topic_facet |
Antarctic Cyanobacteria Life sciences Microbiology Sciences du vivant Microbiologie |
description |
Filamentous cyanobacteria are keystone species in Antarctic lake ecosystems; they are the basis of the simple foodwebs, play a crucial role in biogeochemical cycling and form the structure of benthic microbial mats which act as habitats for other prokaryotic and (micro-eukaryotic biota. Despite this, little is known about their diversity, adaptation and survival strategies in the extreme Antarctic conditions. We studied the uncultivated prokaryotic diversity using a 454 metagenomic analysis at the 16S rRNA level (V1-V3 region) in Continental Antarctic lakes situated along a conductivity gradient (0.014-142.02 mS/cm). The quality and length of the amplicons was analyzed with a custom-made Mothur pipeline and the resulting sequences were mapped against the Greengenes database, which includes CyanoDB. Almost 27% of the sequences could be assigned to the phylum of the cyanobacteria. The most abundant cyanobacteria in the dataset belonged to the genera Microcoleus, Leptolyngbya, Pseudanabaena, Nodularia and Phormidum. Some 16S rRNA types (at the 97% similarity level), such as sequences related to Leptolynbya antarctica, were present in both freshwater and hypersaline lakes. In order to further investigate this distribution, we isolated filaments of Leptolyngbya from seven lakes with conductivities ranging between 26.8 mS/cm and 0.038 mS/cm. The complete 16S rRNA and ITS genes of the isolates were subsequently sequenced. We found several 16S types related to different lineages of filamentous cyanobacteria in the seven lakes that were supported by ITS data. Two 16S types, belonging to a Leptolyngbya antarctica and Leptolyngbya sp., were each present in two different freshwater lakes. Two different 16S types, both belonging to Leptolynbya antarctica were present in a freshwater and hypersaline lake, which indicates a high ‘intraspecific’ molecular diversity. In order to better understand the adaptation and/or wide tolerance to osmotic stress, we are currently performing ecophysiological experiments with these isolates ... |
author2 |
CIP - Centre d'Ingénierie des Protéines - ULiège |
format |
Conference Object |
author |
Obbels, Dagmar Verleyen, Elie Tytgat, Bjorn Elster, Josef Strunecki, Otakar Wilmotte, Annick Willems, Anne Sabbe, Koen Vyverman, Wim |
author_facet |
Obbels, Dagmar Verleyen, Elie Tytgat, Bjorn Elster, Josef Strunecki, Otakar Wilmotte, Annick Willems, Anne Sabbe, Koen Vyverman, Wim |
author_sort |
Obbels, Dagmar |
title |
The diversity and tolerance to osmotic stress of East Antarctic filamentous Cyanobacteria |
title_short |
The diversity and tolerance to osmotic stress of East Antarctic filamentous Cyanobacteria |
title_full |
The diversity and tolerance to osmotic stress of East Antarctic filamentous Cyanobacteria |
title_fullStr |
The diversity and tolerance to osmotic stress of East Antarctic filamentous Cyanobacteria |
title_full_unstemmed |
The diversity and tolerance to osmotic stress of East Antarctic filamentous Cyanobacteria |
title_sort |
diversity and tolerance to osmotic stress of east antarctic filamentous cyanobacteria |
publishDate |
2013 |
url |
https://orbi.uliege.be/handle/2268/153857 |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
11th SCAR Biology Symposium, Barcelona, Spain [ES], 15-19 juillet 2013 |
op_relation |
https://orbi.uliege.be/handle/2268/153857 info:hdl:2268/153857 |
_version_ |
1796946212452040704 |