Late Holocene changes in cyanobacterial community structure in maritime Antarctic lakes
peer reviewed Despite the dominance of cyanobacteria in polar freshwater aquatic ecosystems, little is known about their past biodiversity and response to climate and environmental changes. We explored the use of light microscopy of microfossils, high performance liquid chromatography of the fossil...
Published in: | Journal of Paleolimnology |
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Springer Science & Business Media B.V.
2013
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Online Access: | https://orbi.uliege.be/handle/2268/148513 https://orbi.uliege.be/bitstream/2268/148513/1/BeakfossilFernandez2013.pdf https://doi.org/10.1007/s10933-013-9700-3 |
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ftorbi:oai:orbi.ulg.ac.be:2268/148513 2024-04-21T07:50:46+00:00 Late Holocene changes in cyanobacterial community structure in maritime Antarctic lakes Changements de la structure des communautés de cyanobactéries dans des lacs maritimes antarctiques pendant l'Holocène Fernandez-Carazo, Rafael Verleyen, Elie Hodgson, Dominic A Roberts, Stephen J Waleron, Kzrysztof Vyverman, Wim Wilmotte, Annick CIP - Centre d'Ingénierie des Protéines - ULiège 2013-03 https://orbi.uliege.be/handle/2268/148513 https://orbi.uliege.be/bitstream/2268/148513/1/BeakfossilFernandez2013.pdf https://doi.org/10.1007/s10933-013-9700-3 en eng Springer Science & Business Media B.V. http://link.springer.com/article/10.1007%2Fs10933-013-9700-3 urn:issn:0921-2728 urn:issn:1573-0417 https://orbi.uliege.be/handle/2268/148513 info:hdl:2268/148513 https://orbi.uliege.be/bitstream/2268/148513/1/BeakfossilFernandez2013.pdf doi:10.1007/s10933-013-9700-3 scopus-id:2-s2.0-84877824072 open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess Journal of Paleolimnology, 50, 15-31 (2013-03) fossil DNA sediments cyanobacteria Antarctica maritime lakes Holocene Life sciences Aquatic sciences & oceanology Microbiology Sciences du vivant Sciences aquatiques & océanologie Microbiologie journal article http://purl.org/coar/resource_type/c_6501 info:eu-repo/semantics/article peer reviewed 2013 ftorbi https://doi.org/10.1007/s10933-013-9700-3 2024-03-27T14:56:25Z peer reviewed Despite the dominance of cyanobacteria in polar freshwater aquatic ecosystems, little is known about their past biodiversity and response to climate and environmental changes. We explored the use of light microscopy of microfossils, high performance liquid chromatography of the fossil pigment composition and denaturing gradient gel electrophoresis of fossil 16S rRNA genes to study past and present-day differences in cyanobacterial community structure in response to climate changes in two adjacent maritime Antarctic lakes with contrasting depths (4 and 26 m) and light climates. Light microscopy was of limited use because of degradation of cell structures. Fossil cyanobacterial pigment concentrations were below the detection limits of our method in several sediment samples in the deep lake, but abundant and diverse inthe sediment core from the shallow pond, probably as a consequence of increased light availability and/or a more diverse and abundant benthic cyanobacterial flora. Total carotenoid and chlorophyll concentrations were highest in both lakes between ca. 2,950 and 1,800 cal yr BP, which coincides with the late Holocene climate optimum recognised elsewhere in maritime Antarctica. Cyanobacterial molecular diversity was higher in the top few centimeters of the sediments in both lakes. In deeper sediments, the taxonomic turnover of cyanobacteria appeared to be relatively small in response to past climate anomalies in both lakes, underscoring the broad tolerance of cyanobacteria to environmental variability. This, however, may in part be explained by the low taxonomic resolution obtained with the relatively conserved 16S rRNA gene and/or the preferential preservation of particular taxa. Our results highlight the potential of fossil DNA in lake sediments to study colonization and succession dynamics of lacustrine cyanobacteria and warrant further investigation of the factors that affect preservation of cyanobacterial DNA. HOLANT Article in Journal/Newspaper Antarc* Antarctic Antarctica Antarctique* University of Liège: ORBi (Open Repository and Bibliography) Journal of Paleolimnology 50 1 15 31 |
institution |
Open Polar |
collection |
University of Liège: ORBi (Open Repository and Bibliography) |
op_collection_id |
ftorbi |
language |
English |
topic |
fossil DNA sediments cyanobacteria Antarctica maritime lakes Holocene Life sciences Aquatic sciences & oceanology Microbiology Sciences du vivant Sciences aquatiques & océanologie Microbiologie |
spellingShingle |
fossil DNA sediments cyanobacteria Antarctica maritime lakes Holocene Life sciences Aquatic sciences & oceanology Microbiology Sciences du vivant Sciences aquatiques & océanologie Microbiologie Fernandez-Carazo, Rafael Verleyen, Elie Hodgson, Dominic A Roberts, Stephen J Waleron, Kzrysztof Vyverman, Wim Wilmotte, Annick Late Holocene changes in cyanobacterial community structure in maritime Antarctic lakes |
topic_facet |
fossil DNA sediments cyanobacteria Antarctica maritime lakes Holocene Life sciences Aquatic sciences & oceanology Microbiology Sciences du vivant Sciences aquatiques & océanologie Microbiologie |
description |
peer reviewed Despite the dominance of cyanobacteria in polar freshwater aquatic ecosystems, little is known about their past biodiversity and response to climate and environmental changes. We explored the use of light microscopy of microfossils, high performance liquid chromatography of the fossil pigment composition and denaturing gradient gel electrophoresis of fossil 16S rRNA genes to study past and present-day differences in cyanobacterial community structure in response to climate changes in two adjacent maritime Antarctic lakes with contrasting depths (4 and 26 m) and light climates. Light microscopy was of limited use because of degradation of cell structures. Fossil cyanobacterial pigment concentrations were below the detection limits of our method in several sediment samples in the deep lake, but abundant and diverse inthe sediment core from the shallow pond, probably as a consequence of increased light availability and/or a more diverse and abundant benthic cyanobacterial flora. Total carotenoid and chlorophyll concentrations were highest in both lakes between ca. 2,950 and 1,800 cal yr BP, which coincides with the late Holocene climate optimum recognised elsewhere in maritime Antarctica. Cyanobacterial molecular diversity was higher in the top few centimeters of the sediments in both lakes. In deeper sediments, the taxonomic turnover of cyanobacteria appeared to be relatively small in response to past climate anomalies in both lakes, underscoring the broad tolerance of cyanobacteria to environmental variability. This, however, may in part be explained by the low taxonomic resolution obtained with the relatively conserved 16S rRNA gene and/or the preferential preservation of particular taxa. Our results highlight the potential of fossil DNA in lake sediments to study colonization and succession dynamics of lacustrine cyanobacteria and warrant further investigation of the factors that affect preservation of cyanobacterial DNA. HOLANT |
author2 |
CIP - Centre d'Ingénierie des Protéines - ULiège |
format |
Article in Journal/Newspaper |
author |
Fernandez-Carazo, Rafael Verleyen, Elie Hodgson, Dominic A Roberts, Stephen J Waleron, Kzrysztof Vyverman, Wim Wilmotte, Annick |
author_facet |
Fernandez-Carazo, Rafael Verleyen, Elie Hodgson, Dominic A Roberts, Stephen J Waleron, Kzrysztof Vyverman, Wim Wilmotte, Annick |
author_sort |
Fernandez-Carazo, Rafael |
title |
Late Holocene changes in cyanobacterial community structure in maritime Antarctic lakes |
title_short |
Late Holocene changes in cyanobacterial community structure in maritime Antarctic lakes |
title_full |
Late Holocene changes in cyanobacterial community structure in maritime Antarctic lakes |
title_fullStr |
Late Holocene changes in cyanobacterial community structure in maritime Antarctic lakes |
title_full_unstemmed |
Late Holocene changes in cyanobacterial community structure in maritime Antarctic lakes |
title_sort |
late holocene changes in cyanobacterial community structure in maritime antarctic lakes |
publisher |
Springer Science & Business Media B.V. |
publishDate |
2013 |
url |
https://orbi.uliege.be/handle/2268/148513 https://orbi.uliege.be/bitstream/2268/148513/1/BeakfossilFernandez2013.pdf https://doi.org/10.1007/s10933-013-9700-3 |
genre |
Antarc* Antarctic Antarctica Antarctique* |
genre_facet |
Antarc* Antarctic Antarctica Antarctique* |
op_source |
Journal of Paleolimnology, 50, 15-31 (2013-03) |
op_relation |
http://link.springer.com/article/10.1007%2Fs10933-013-9700-3 urn:issn:0921-2728 urn:issn:1573-0417 https://orbi.uliege.be/handle/2268/148513 info:hdl:2268/148513 https://orbi.uliege.be/bitstream/2268/148513/1/BeakfossilFernandez2013.pdf doi:10.1007/s10933-013-9700-3 scopus-id:2-s2.0-84877824072 |
op_rights |
open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1007/s10933-013-9700-3 |
container_title |
Journal of Paleolimnology |
container_volume |
50 |
container_issue |
1 |
container_start_page |
15 |
op_container_end_page |
31 |
_version_ |
1796934357931261952 |