Experimental challenges with Renibacterium salmoninarum in Arctic charr Salvelinus alpinus
Arctic charr Salvelinus alpinus L. is an important species in Icelandic aquaculture and the most common wild salmonid in Iceland. A study on the course of infection with the bacterium Renibacterium salmoninarum was conducted using 3 different challenge methods in brackish and fresh water. Bacterial...
Published in: | Diseases of Aquatic Organisms |
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Format: | Article in Journal/Newspaper |
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Inter-Research Science Center
2017
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Online Access: | https://hdl.handle.net/20.500.11815/619 https://doi.org/10.3354/dao03107 |
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ftopinvisindi:oai:opinvisindi.is:20.500.11815/619 2023-05-15T13:08:38+02:00 Experimental challenges with Renibacterium salmoninarum in Arctic charr Salvelinus alpinus Guðmundsdóttir, Sigríður Kristmundsson, Árni Árnason, Ívar Örn Tilraunastöð í meinafræði að Keldum (HÍ) Institute for Experimental Pathology, Keldur (UI) Háskóli Íslands University of Iceland 2017-03-30 21-30 https://hdl.handle.net/20.500.11815/619 https://doi.org/10.3354/dao03107 en eng Inter-Research Science Center Diseases of Aquatic Organisms;124(1) http://www.int-res.com/articles/dao_oa/d124p021.pdf Gudmundsdóttir, S., Kristmundsson, Á., & Árnason, Í. (2017). Experimental challenges with Renibacterium salmoninarum in Arctic charr Salvelinus alpinus. Diseases of Aquatic Organisms, 124(1), 21-30. doi:10.3354/dao03107 0177-5103 1616-1580 (eISSN) https://hdl.handle.net/20.500.11815/619 Diseases of Aquatic Organisms doi:10.3354/dao03107 info:eu-repo/semantics/openAccess Renibacterium salmoninarum Salvelinus alpinus Challenge methods Infection state Diagnostic assays Arctic charr Bleikja Gerlar Bakteríusjúkdómar info:eu-repo/semantics/article 2017 ftopinvisindi https://doi.org/20.500.11815/619 https://doi.org/10.3354/dao03107 2022-11-18T06:51:36Z Arctic charr Salvelinus alpinus L. is an important species in Icelandic aquaculture and the most common wild salmonid in Iceland. A study on the course of infection with the bacterium Renibacterium salmoninarum was conducted using 3 different challenge methods in brackish and fresh water. Bacterial isolation, ELISA and PCR tests were used for detection of the bacterium in multiple organ samples. In an experiment, run for 34 wk in brackish water, infection was established by intraperitoneal injection with 5 × 106 colony forming units (CFU) fish-1. There were external and internal symptoms of bacterial kidney disease (BKD) and mortalities between 6 and 13 wk after injection. A cohabitation trial was run simultaneously and infection was well established after 4 wk, as demonstrated by the detection methods applied. Symptoms of BKD were not seen and all but 1 cohabitant survived. In a separate experiment, infection was established by pumping a fixed amount of water from a tank with fingerlings infected by intraperitoneal injection into tanks with naïve fish, in fresh or brackish water, for 6 wk. Fish in the inflow tanks were reared for an additional 3 wk. There were neither macroscopic symptoms nor mortalities. ELISA and PCR tests showed that infection started to take hold after 3 wk. The challenge trials demonstrated that Arctic charr is susceptible to R. salmoninarum. Cohabitation and inflow of water from tanks with infected fish provide useful models for further studies on R. salmoninarum infection acquired in a natural way in Arctic charr. The authors are indebted to Islands-bleikja at Grindavik (Samherji hf., Akureyri, Iceland) for providing the fish used in the study, to the staff at Sudurnes Science and Learning Centre for attendance to the experimental fish and to Sigurour Snorrason and Asthildur Erlingsdottir for constructive criticism on the manuscript. This work received grants from the AVS R&D Fund of Ministry of Fisheries and Agriculture in Iceland no. R 10 0093-10 and R 13 074-13. Peer Reviewed Article in Journal/Newspaper Akureyri Akureyri Akureyri Arctic Arctic charr Arctic Iceland Salvelinus alpinus Opin vísindi (Iceland) Akureyri Arctic Diseases of Aquatic Organisms 124 1 21 30 |
institution |
Open Polar |
collection |
Opin vísindi (Iceland) |
op_collection_id |
ftopinvisindi |
language |
English |
topic |
Renibacterium salmoninarum Salvelinus alpinus Challenge methods Infection state Diagnostic assays Arctic charr Bleikja Gerlar Bakteríusjúkdómar |
spellingShingle |
Renibacterium salmoninarum Salvelinus alpinus Challenge methods Infection state Diagnostic assays Arctic charr Bleikja Gerlar Bakteríusjúkdómar Guðmundsdóttir, Sigríður Kristmundsson, Árni Árnason, Ívar Örn Experimental challenges with Renibacterium salmoninarum in Arctic charr Salvelinus alpinus |
topic_facet |
Renibacterium salmoninarum Salvelinus alpinus Challenge methods Infection state Diagnostic assays Arctic charr Bleikja Gerlar Bakteríusjúkdómar |
description |
Arctic charr Salvelinus alpinus L. is an important species in Icelandic aquaculture and the most common wild salmonid in Iceland. A study on the course of infection with the bacterium Renibacterium salmoninarum was conducted using 3 different challenge methods in brackish and fresh water. Bacterial isolation, ELISA and PCR tests were used for detection of the bacterium in multiple organ samples. In an experiment, run for 34 wk in brackish water, infection was established by intraperitoneal injection with 5 × 106 colony forming units (CFU) fish-1. There were external and internal symptoms of bacterial kidney disease (BKD) and mortalities between 6 and 13 wk after injection. A cohabitation trial was run simultaneously and infection was well established after 4 wk, as demonstrated by the detection methods applied. Symptoms of BKD were not seen and all but 1 cohabitant survived. In a separate experiment, infection was established by pumping a fixed amount of water from a tank with fingerlings infected by intraperitoneal injection into tanks with naïve fish, in fresh or brackish water, for 6 wk. Fish in the inflow tanks were reared for an additional 3 wk. There were neither macroscopic symptoms nor mortalities. ELISA and PCR tests showed that infection started to take hold after 3 wk. The challenge trials demonstrated that Arctic charr is susceptible to R. salmoninarum. Cohabitation and inflow of water from tanks with infected fish provide useful models for further studies on R. salmoninarum infection acquired in a natural way in Arctic charr. The authors are indebted to Islands-bleikja at Grindavik (Samherji hf., Akureyri, Iceland) for providing the fish used in the study, to the staff at Sudurnes Science and Learning Centre for attendance to the experimental fish and to Sigurour Snorrason and Asthildur Erlingsdottir for constructive criticism on the manuscript. This work received grants from the AVS R&D Fund of Ministry of Fisheries and Agriculture in Iceland no. R 10 0093-10 and R 13 074-13. Peer Reviewed |
author2 |
Tilraunastöð í meinafræði að Keldum (HÍ) Institute for Experimental Pathology, Keldur (UI) Háskóli Íslands University of Iceland |
format |
Article in Journal/Newspaper |
author |
Guðmundsdóttir, Sigríður Kristmundsson, Árni Árnason, Ívar Örn |
author_facet |
Guðmundsdóttir, Sigríður Kristmundsson, Árni Árnason, Ívar Örn |
author_sort |
Guðmundsdóttir, Sigríður |
title |
Experimental challenges with Renibacterium salmoninarum in Arctic charr Salvelinus alpinus |
title_short |
Experimental challenges with Renibacterium salmoninarum in Arctic charr Salvelinus alpinus |
title_full |
Experimental challenges with Renibacterium salmoninarum in Arctic charr Salvelinus alpinus |
title_fullStr |
Experimental challenges with Renibacterium salmoninarum in Arctic charr Salvelinus alpinus |
title_full_unstemmed |
Experimental challenges with Renibacterium salmoninarum in Arctic charr Salvelinus alpinus |
title_sort |
experimental challenges with renibacterium salmoninarum in arctic charr salvelinus alpinus |
publisher |
Inter-Research Science Center |
publishDate |
2017 |
url |
https://hdl.handle.net/20.500.11815/619 https://doi.org/10.3354/dao03107 |
geographic |
Akureyri Arctic |
geographic_facet |
Akureyri Arctic |
genre |
Akureyri Akureyri Akureyri Arctic Arctic charr Arctic Iceland Salvelinus alpinus |
genre_facet |
Akureyri Akureyri Akureyri Arctic Arctic charr Arctic Iceland Salvelinus alpinus |
op_relation |
Diseases of Aquatic Organisms;124(1) http://www.int-res.com/articles/dao_oa/d124p021.pdf Gudmundsdóttir, S., Kristmundsson, Á., & Árnason, Í. (2017). Experimental challenges with Renibacterium salmoninarum in Arctic charr Salvelinus alpinus. Diseases of Aquatic Organisms, 124(1), 21-30. doi:10.3354/dao03107 0177-5103 1616-1580 (eISSN) https://hdl.handle.net/20.500.11815/619 Diseases of Aquatic Organisms doi:10.3354/dao03107 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/20.500.11815/619 https://doi.org/10.3354/dao03107 |
container_title |
Diseases of Aquatic Organisms |
container_volume |
124 |
container_issue |
1 |
container_start_page |
21 |
op_container_end_page |
30 |
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1766104671460524032 |