Differential gene expression during early development in recently evolved and sympatric Arctic charr morphs
Phenotypic differences between closely related taxa or populations can arise through genetic variation or be environmentally induced, leading to altered transcription of genes during development. Comparative developmental studies of closely related species or variable populations within species can...
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ftopinvisindi:oai:opinvisindi.is:20.500.11815/584 2024-09-15T17:50:54+00:00 Differential gene expression during early development in recently evolved and sympatric Arctic charr morphs Guðbrandsson, Jóhannes Franzdottir, Sigridur Rut Kristjansson, Bjarni K. Pashay Ahi, Ehsan Maier, Valerie H. Kapralova, Kalina Snorrason, Sigurður S. Jónsson, Zophonías Oddur Palsson, Arnar Líf- og umhverfisvísindadeild (HÍ) Faculty of Life and Environmental Sciences (UI) Líf- og umhverfisvísindastofnun (HÍ) Institute of Life and Environmental Sciences (UI) Lífvísindasetur (HÍ) Biomedical Center (UI) Fiskeldis- og fiskalíffræðideild (HH) Department of Aquaculture and Fish Biology (HUC) Verkfræði- og náttúruvísindasvið (HÍ) School of Engineering and Natural Sciences (UI) Háskóli Íslands University of Iceland Háskólinn á Hólum Hólar University College 2018-02-07 e4345 https://hdl.handle.net/20.500.11815/584 https://doi.org/10.7717/peerj.4345 en eng PeerJ PeerJ;6 Guðbrandsson J, Franzdóttir SR, Kristjánsson BK, Ahi EP, Maier VH, Kapralova KH, Snorrason SS, Jónsson ZO, Pálsson A. (2018) Differential gene expression during early development in recently evolved and sympatric Arctic charr morphs. PeerJ 6:e4345 https://doi.org/10.7717/peerj.4345 2167-8359 https://hdl.handle.net/20.500.11815/584 PeerJ doi:10.7717/peerj.4345 info:eu-repo/semantics/openAccess Developmental Biology Evolutionary Studies Genomics Molecular Biology Freshwater Biology Arctic charr Þróun lífsins Erfðafræði Sameindalíffræði Vatnalíffræði Bleikja info:eu-repo/semantics/article 2018 ftopinvisindi https://doi.org/20.500.11815/58410.7717/peerj.4345 2024-07-09T03:01:56Z Phenotypic differences between closely related taxa or populations can arise through genetic variation or be environmentally induced, leading to altered transcription of genes during development. Comparative developmental studies of closely related species or variable populations within species can help to elucidate the molecular mechanisms related to evolutionary divergence and speciation. Studies of Arctic charr (Salvelinus alpinus) and related salmonids have revealed considerable phenotypic variation among populations and in Arctic charr many cases of extensive variation within lakes (resource polymorphism) have been recorded. One example is the four Arctic charr morphs in the ∼10,000 year old Lake Thingvallavatn, which differ in numerous morphological and life history traits. We set out to investigate the molecular and developmental roots of this polymorphism by studying gene expression in embryos of three of the morphs reared in a common garden set-up. We performed RNA-sequencing, de-novo transcriptome assembly and compared gene expression among morphs during an important timeframe in early development, i.e., preceding the formation of key trophic structures. Expectedly, developmental time was the predominant explanatory variable. As the data were affected by some form of RNA-degradation even though all samples passed quality control testing, an estimate of 3′-bias was the second most common explanatory variable. Importantly, morph, both as an independent variable and as interaction with developmental time, affected the expression of numerous transcripts. Transcripts with morph effect, separated the three morphs at the expression level, with the two benthic morphs being more similar. However, Gene Ontology analyses did not reveal clear functional enrichment of transcripts between groups. Verification via qPCR confirmed differential expression of several genes between the morphs, including regulatory genes such as AT-Rich Interaction Domain 4A (arid4a) and translin (tsn). The data are consistent with a ... Article in Journal/Newspaper Arctic Arctic charr Salvelinus alpinus Opin vísindi (Iceland) PeerJ 6 e4345 |
institution |
Open Polar |
collection |
Opin vísindi (Iceland) |
op_collection_id |
ftopinvisindi |
language |
English |
topic |
Developmental Biology Evolutionary Studies Genomics Molecular Biology Freshwater Biology Arctic charr Þróun lífsins Erfðafræði Sameindalíffræði Vatnalíffræði Bleikja |
spellingShingle |
Developmental Biology Evolutionary Studies Genomics Molecular Biology Freshwater Biology Arctic charr Þróun lífsins Erfðafræði Sameindalíffræði Vatnalíffræði Bleikja Guðbrandsson, Jóhannes Franzdottir, Sigridur Rut Kristjansson, Bjarni K. Pashay Ahi, Ehsan Maier, Valerie H. Kapralova, Kalina Snorrason, Sigurður S. Jónsson, Zophonías Oddur Palsson, Arnar Differential gene expression during early development in recently evolved and sympatric Arctic charr morphs |
topic_facet |
Developmental Biology Evolutionary Studies Genomics Molecular Biology Freshwater Biology Arctic charr Þróun lífsins Erfðafræði Sameindalíffræði Vatnalíffræði Bleikja |
description |
Phenotypic differences between closely related taxa or populations can arise through genetic variation or be environmentally induced, leading to altered transcription of genes during development. Comparative developmental studies of closely related species or variable populations within species can help to elucidate the molecular mechanisms related to evolutionary divergence and speciation. Studies of Arctic charr (Salvelinus alpinus) and related salmonids have revealed considerable phenotypic variation among populations and in Arctic charr many cases of extensive variation within lakes (resource polymorphism) have been recorded. One example is the four Arctic charr morphs in the ∼10,000 year old Lake Thingvallavatn, which differ in numerous morphological and life history traits. We set out to investigate the molecular and developmental roots of this polymorphism by studying gene expression in embryos of three of the morphs reared in a common garden set-up. We performed RNA-sequencing, de-novo transcriptome assembly and compared gene expression among morphs during an important timeframe in early development, i.e., preceding the formation of key trophic structures. Expectedly, developmental time was the predominant explanatory variable. As the data were affected by some form of RNA-degradation even though all samples passed quality control testing, an estimate of 3′-bias was the second most common explanatory variable. Importantly, morph, both as an independent variable and as interaction with developmental time, affected the expression of numerous transcripts. Transcripts with morph effect, separated the three morphs at the expression level, with the two benthic morphs being more similar. However, Gene Ontology analyses did not reveal clear functional enrichment of transcripts between groups. Verification via qPCR confirmed differential expression of several genes between the morphs, including regulatory genes such as AT-Rich Interaction Domain 4A (arid4a) and translin (tsn). The data are consistent with a ... |
author2 |
Líf- og umhverfisvísindadeild (HÍ) Faculty of Life and Environmental Sciences (UI) Líf- og umhverfisvísindastofnun (HÍ) Institute of Life and Environmental Sciences (UI) Lífvísindasetur (HÍ) Biomedical Center (UI) Fiskeldis- og fiskalíffræðideild (HH) Department of Aquaculture and Fish Biology (HUC) Verkfræði- og náttúruvísindasvið (HÍ) School of Engineering and Natural Sciences (UI) Háskóli Íslands University of Iceland Háskólinn á Hólum Hólar University College |
format |
Article in Journal/Newspaper |
author |
Guðbrandsson, Jóhannes Franzdottir, Sigridur Rut Kristjansson, Bjarni K. Pashay Ahi, Ehsan Maier, Valerie H. Kapralova, Kalina Snorrason, Sigurður S. Jónsson, Zophonías Oddur Palsson, Arnar |
author_facet |
Guðbrandsson, Jóhannes Franzdottir, Sigridur Rut Kristjansson, Bjarni K. Pashay Ahi, Ehsan Maier, Valerie H. Kapralova, Kalina Snorrason, Sigurður S. Jónsson, Zophonías Oddur Palsson, Arnar |
author_sort |
Guðbrandsson, Jóhannes |
title |
Differential gene expression during early development in recently evolved and sympatric Arctic charr morphs |
title_short |
Differential gene expression during early development in recently evolved and sympatric Arctic charr morphs |
title_full |
Differential gene expression during early development in recently evolved and sympatric Arctic charr morphs |
title_fullStr |
Differential gene expression during early development in recently evolved and sympatric Arctic charr morphs |
title_full_unstemmed |
Differential gene expression during early development in recently evolved and sympatric Arctic charr morphs |
title_sort |
differential gene expression during early development in recently evolved and sympatric arctic charr morphs |
publisher |
PeerJ |
publishDate |
2018 |
url |
https://hdl.handle.net/20.500.11815/584 https://doi.org/10.7717/peerj.4345 |
genre |
Arctic Arctic charr Salvelinus alpinus |
genre_facet |
Arctic Arctic charr Salvelinus alpinus |
op_relation |
PeerJ;6 Guðbrandsson J, Franzdóttir SR, Kristjánsson BK, Ahi EP, Maier VH, Kapralova KH, Snorrason SS, Jónsson ZO, Pálsson A. (2018) Differential gene expression during early development in recently evolved and sympatric Arctic charr morphs. PeerJ 6:e4345 https://doi.org/10.7717/peerj.4345 2167-8359 https://hdl.handle.net/20.500.11815/584 PeerJ doi:10.7717/peerj.4345 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/20.500.11815/58410.7717/peerj.4345 |
container_title |
PeerJ |
container_volume |
6 |
container_start_page |
e4345 |
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1810292709552816128 |