Can Lichens Serve as Hosts for Pseudomonas syringae in Icelandic Habitats? : Isolation, Plant-pathogenic Traits, and Metabolomic Insights into the Role of P. syringae in Icelandic Peltigera Lichens
Pseudomonas syringae, traditionally recognized as a plant pathogen, has a broader ecological role beyond agriculture. In diverse non-agricultural environments, researchers have isolated the bacterium revealing non-pathogenic behavior and significant roles, often associated with its ice nucleation pr...
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University of Akureyri
2024
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ftopinvisindi:oai:opinvisindi.is:20.500.11815/4923 2024-06-23T07:54:00+00:00 Can Lichens Serve as Hosts for Pseudomonas syringae in Icelandic Habitats? : Isolation, Plant-pathogenic Traits, and Metabolomic Insights into the Role of P. syringae in Icelandic Peltigera Lichens Carrera, Natalia Ramirez Vilhelmsson, Oddur Þór Sigurbjörnsdóttir, Margrét Auður Morris, Cindy E. Berge, Odile Heiðmarsson, Starri Jackson, Robert W. University of Akureyri 2024 151 15652609 https://hdl.handle.net/20.500.11815/4923 en eng University of Akureyri Carrera , N R 2024 , ' Can Lichens Serve as Hosts for Pseudomonas syringae in Icelandic Habitats? Isolation, Plant-pathogenic Traits, and Metabolomic Insights into the Role of P. syringae in Icelandic Peltigera Lichens ' , Doctor , University of Akureyri , Akureyri . 978-9935-505-19-4 222919459 6720f5b3-8cd2-43bf-a370-59eaca9d1acc https://hdl.handle.net/20.500.11815/4923 info:eu-repo/semantics/openAccess Gerlar Örverur Plant pathogenicity Iceland Untargeted metabolomics Bacterial phytopathogen Non-agricultural habitat /dk/atira/pure/researchoutput/researchoutputtypes/thesis/doc 2024 ftopinvisindi https://doi.org/20.500.11815/4923 2024-06-03T23:36:06Z Pseudomonas syringae, traditionally recognized as a plant pathogen, has a broader ecological role beyond agriculture. In diverse non-agricultural environments, researchers have isolated the bacterium revealing non-pathogenic behavior and significant roles, often associated with its ice nucleation property. The primary objective of this project was to achieve the first isolation of P. syringae from lichens. In Paper I, we detail the isolation process from a specific lichen genus, Peltigera, after screening 10 different lichen genera. The analysis extends to studying the P. syringae population within the same sampling points alongside Peltigera, moss, and tracheophytes when feasible. Following the isolation of this potential plant pathogen, the logical progression led to Paper II, where we explored the fitness and pathology of selected P. syringae strains, predominantly from more aggressive phylogroups, across ten different plant species, primarily crops. The results showed similar pathogenicity in some lichen strains compared with epidemic ones and a similar fitness in 8 out of 10 plant species tested. The final project phase aimed to understand the exclusive isolation of P. syringae from a single genus. Paper III, adopting a metabolomics approach, analyzed differences in the profiles of Peltigera and non-Peltigera lichen genera that may influence P. syringae presence. Peltigera's overall profile shows a higher chemical investment, focusing on the production of certain compounds. Kinetics and inhibition analyses suggest a slightly increased growth of P. syringae in Peltigera media, though not considered decisive. The study also outlines specific compounds present or absent in Peltigera, including the absence of ubiquinones, recognized for their resistance role against P. syringae in various plant species. Furthermore, experiments with P. syringae isolated in Iceland gain significance through collaboration with INRAE (Paper IV). Comparisons with a global database reveal that this Icelandic P. syringae population ... Other/Unknown Material Iceland Opin vísindi (Iceland) |
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Opin vísindi (Iceland) |
op_collection_id |
ftopinvisindi |
language |
English |
topic |
Gerlar Örverur Plant pathogenicity Iceland Untargeted metabolomics Bacterial phytopathogen Non-agricultural habitat |
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Gerlar Örverur Plant pathogenicity Iceland Untargeted metabolomics Bacterial phytopathogen Non-agricultural habitat Carrera, Natalia Ramirez Can Lichens Serve as Hosts for Pseudomonas syringae in Icelandic Habitats? : Isolation, Plant-pathogenic Traits, and Metabolomic Insights into the Role of P. syringae in Icelandic Peltigera Lichens |
topic_facet |
Gerlar Örverur Plant pathogenicity Iceland Untargeted metabolomics Bacterial phytopathogen Non-agricultural habitat |
description |
Pseudomonas syringae, traditionally recognized as a plant pathogen, has a broader ecological role beyond agriculture. In diverse non-agricultural environments, researchers have isolated the bacterium revealing non-pathogenic behavior and significant roles, often associated with its ice nucleation property. The primary objective of this project was to achieve the first isolation of P. syringae from lichens. In Paper I, we detail the isolation process from a specific lichen genus, Peltigera, after screening 10 different lichen genera. The analysis extends to studying the P. syringae population within the same sampling points alongside Peltigera, moss, and tracheophytes when feasible. Following the isolation of this potential plant pathogen, the logical progression led to Paper II, where we explored the fitness and pathology of selected P. syringae strains, predominantly from more aggressive phylogroups, across ten different plant species, primarily crops. The results showed similar pathogenicity in some lichen strains compared with epidemic ones and a similar fitness in 8 out of 10 plant species tested. The final project phase aimed to understand the exclusive isolation of P. syringae from a single genus. Paper III, adopting a metabolomics approach, analyzed differences in the profiles of Peltigera and non-Peltigera lichen genera that may influence P. syringae presence. Peltigera's overall profile shows a higher chemical investment, focusing on the production of certain compounds. Kinetics and inhibition analyses suggest a slightly increased growth of P. syringae in Peltigera media, though not considered decisive. The study also outlines specific compounds present or absent in Peltigera, including the absence of ubiquinones, recognized for their resistance role against P. syringae in various plant species. Furthermore, experiments with P. syringae isolated in Iceland gain significance through collaboration with INRAE (Paper IV). Comparisons with a global database reveal that this Icelandic P. syringae population ... |
author2 |
Vilhelmsson, Oddur Þór Sigurbjörnsdóttir, Margrét Auður Morris, Cindy E. Berge, Odile Heiðmarsson, Starri Jackson, Robert W. University of Akureyri |
format |
Other/Unknown Material |
author |
Carrera, Natalia Ramirez |
author_facet |
Carrera, Natalia Ramirez |
author_sort |
Carrera, Natalia Ramirez |
title |
Can Lichens Serve as Hosts for Pseudomonas syringae in Icelandic Habitats? : Isolation, Plant-pathogenic Traits, and Metabolomic Insights into the Role of P. syringae in Icelandic Peltigera Lichens |
title_short |
Can Lichens Serve as Hosts for Pseudomonas syringae in Icelandic Habitats? : Isolation, Plant-pathogenic Traits, and Metabolomic Insights into the Role of P. syringae in Icelandic Peltigera Lichens |
title_full |
Can Lichens Serve as Hosts for Pseudomonas syringae in Icelandic Habitats? : Isolation, Plant-pathogenic Traits, and Metabolomic Insights into the Role of P. syringae in Icelandic Peltigera Lichens |
title_fullStr |
Can Lichens Serve as Hosts for Pseudomonas syringae in Icelandic Habitats? : Isolation, Plant-pathogenic Traits, and Metabolomic Insights into the Role of P. syringae in Icelandic Peltigera Lichens |
title_full_unstemmed |
Can Lichens Serve as Hosts for Pseudomonas syringae in Icelandic Habitats? : Isolation, Plant-pathogenic Traits, and Metabolomic Insights into the Role of P. syringae in Icelandic Peltigera Lichens |
title_sort |
can lichens serve as hosts for pseudomonas syringae in icelandic habitats? : isolation, plant-pathogenic traits, and metabolomic insights into the role of p. syringae in icelandic peltigera lichens |
publisher |
University of Akureyri |
publishDate |
2024 |
url |
https://hdl.handle.net/20.500.11815/4923 |
genre |
Iceland |
genre_facet |
Iceland |
op_relation |
Carrera , N R 2024 , ' Can Lichens Serve as Hosts for Pseudomonas syringae in Icelandic Habitats? Isolation, Plant-pathogenic Traits, and Metabolomic Insights into the Role of P. syringae in Icelandic Peltigera Lichens ' , Doctor , University of Akureyri , Akureyri . 978-9935-505-19-4 222919459 6720f5b3-8cd2-43bf-a370-59eaca9d1acc https://hdl.handle.net/20.500.11815/4923 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/20.500.11815/4923 |
_version_ |
1802645929971417088 |