Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland
Twenty-eight sponge specimens were collected at a shallow water hydrothermal vent site north of Iceland. Extracts were prepared and tested in vitro for cytotoxic activity, and eight of them were shown to be cytotoxic. A mass spectrometry (MS)-based metabolomics approach was used to determine the che...
Published in: | Marine Drugs |
---|---|
Main Authors: | , , , , , , , , |
Other Authors: | , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MDPI AG
2017
|
Subjects: | |
Online Access: | https://hdl.handle.net/20.500.11815/340 https://doi.org/10.3390/md15020052 |
id |
ftopinvisindi:oai:opinvisindi.is:20.500.11815/340 |
---|---|
record_format |
openpolar |
spelling |
ftopinvisindi:oai:opinvisindi.is:20.500.11815/340 2024-09-15T18:13:20+00:00 Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland Einarsdóttir, Eydís Magnúsdóttir, Manuela Astarita, Giuseppe Köck, Matthias Ögmundsdóttir, Helga M. Þorsteinsdóttir, Margrét Rapp, Hans Omarsdottir, Sesselja Paglia, Giuseppe Lyfjafræðideild (HÍ) Faculty of Pharmaceutical Sciences (UI) Rannsóknarsetur í kerfislíffræði (HÍ) Center for Systems Biology (UI) Læknadeild (HÍ) Faculty of Medicine (UI) Heilbrigðisvísindasvið (HÍ) School of Health Sciences (UI) Verkfræði- og náttúruvísindasvið (HÍ) School of Engineering and Natural Sciences (UI) Háskóli Íslands University of Iceland 2017-02-22 52 https://hdl.handle.net/20.500.11815/340 https://doi.org/10.3390/md15020052 en eng MDPI AG Marine Drugs;15(3) Einarsdottir E, Magnusdottir M, Astarita G, Köck M, Ögmundsdottir HM, Thorsteinsdottir M, Rapp HT, Omarsdottir S, Paglia G. Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland. Marine Drugs. 2017; 15(2):52. doi:10.3390/md15020052 1660-3397 https://hdl.handle.net/20.500.11815/340 Marine Drugs doi:10.3390/md15020052 info:eu-repo/semantics/openAccess Marine natural products Sponges Metabolomics Ion mobility Haliclona rosea Sjávarafurðir Svampdýr Efnaskipti info:eu-repo/semantics/article 2017 ftopinvisindi https://doi.org/20.500.11815/34010.3390/md15020052 2024-07-09T03:01:56Z Twenty-eight sponge specimens were collected at a shallow water hydrothermal vent site north of Iceland. Extracts were prepared and tested in vitro for cytotoxic activity, and eight of them were shown to be cytotoxic. A mass spectrometry (MS)-based metabolomics approach was used to determine the chemical composition of the extracts. This analysis highlighted clear differences in the metabolomes of three sponge specimens, and all of them were identified as Haliclona (Rhizoniera) rosea (Bowerbank, 1866). Therefore, these specimens were selected for further investigation. Haliclona rosea metabolomes contained a class of potential key compounds, the 3-alkyl pyridine alkaloids (3-APA) responsible for the cytotoxic activity of the fractions. Several 3-APA compounds were tentatively identified including haliclamines, cyclostellettamines, viscosalines and viscosamines. Among these compounds, cyclostellettamine P was tentatively identified for the first time by using ion mobility MS in time-aligned parallel (TAP) fragmentation mode. In this work, we show the potential of applying metabolomics strategies and in particular the utility of coupling ion mobility with MS for the molecular characterization of sponge specimens. The authors would like to thank Erlendur Bogason for his assistance in sample collection as well as being the "protector" of Strytan, the hydrothermal vent site. This project was funded by the Eimskip University Fund, The University of Iceland Research Fund, and the Icelandic Research Fund (grant no.: 110403021). Peer Reviewed Article in Journal/Newspaper Iceland Opin vísindi (Iceland) Marine Drugs 15 2 52 |
institution |
Open Polar |
collection |
Opin vísindi (Iceland) |
op_collection_id |
ftopinvisindi |
language |
English |
topic |
Marine natural products Sponges Metabolomics Ion mobility Haliclona rosea Sjávarafurðir Svampdýr Efnaskipti |
spellingShingle |
Marine natural products Sponges Metabolomics Ion mobility Haliclona rosea Sjávarafurðir Svampdýr Efnaskipti Einarsdóttir, Eydís Magnúsdóttir, Manuela Astarita, Giuseppe Köck, Matthias Ögmundsdóttir, Helga M. Þorsteinsdóttir, Margrét Rapp, Hans Omarsdottir, Sesselja Paglia, Giuseppe Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland |
topic_facet |
Marine natural products Sponges Metabolomics Ion mobility Haliclona rosea Sjávarafurðir Svampdýr Efnaskipti |
description |
Twenty-eight sponge specimens were collected at a shallow water hydrothermal vent site north of Iceland. Extracts were prepared and tested in vitro for cytotoxic activity, and eight of them were shown to be cytotoxic. A mass spectrometry (MS)-based metabolomics approach was used to determine the chemical composition of the extracts. This analysis highlighted clear differences in the metabolomes of three sponge specimens, and all of them were identified as Haliclona (Rhizoniera) rosea (Bowerbank, 1866). Therefore, these specimens were selected for further investigation. Haliclona rosea metabolomes contained a class of potential key compounds, the 3-alkyl pyridine alkaloids (3-APA) responsible for the cytotoxic activity of the fractions. Several 3-APA compounds were tentatively identified including haliclamines, cyclostellettamines, viscosalines and viscosamines. Among these compounds, cyclostellettamine P was tentatively identified for the first time by using ion mobility MS in time-aligned parallel (TAP) fragmentation mode. In this work, we show the potential of applying metabolomics strategies and in particular the utility of coupling ion mobility with MS for the molecular characterization of sponge specimens. The authors would like to thank Erlendur Bogason for his assistance in sample collection as well as being the "protector" of Strytan, the hydrothermal vent site. This project was funded by the Eimskip University Fund, The University of Iceland Research Fund, and the Icelandic Research Fund (grant no.: 110403021). Peer Reviewed |
author2 |
Lyfjafræðideild (HÍ) Faculty of Pharmaceutical Sciences (UI) Rannsóknarsetur í kerfislíffræði (HÍ) Center for Systems Biology (UI) Læknadeild (HÍ) Faculty of Medicine (UI) Heilbrigðisvísindasvið (HÍ) School of Health Sciences (UI) Verkfræði- og náttúruvísindasvið (HÍ) School of Engineering and Natural Sciences (UI) Háskóli Íslands University of Iceland |
format |
Article in Journal/Newspaper |
author |
Einarsdóttir, Eydís Magnúsdóttir, Manuela Astarita, Giuseppe Köck, Matthias Ögmundsdóttir, Helga M. Þorsteinsdóttir, Margrét Rapp, Hans Omarsdottir, Sesselja Paglia, Giuseppe |
author_facet |
Einarsdóttir, Eydís Magnúsdóttir, Manuela Astarita, Giuseppe Köck, Matthias Ögmundsdóttir, Helga M. Þorsteinsdóttir, Margrét Rapp, Hans Omarsdottir, Sesselja Paglia, Giuseppe |
author_sort |
Einarsdóttir, Eydís |
title |
Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland |
title_short |
Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland |
title_full |
Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland |
title_fullStr |
Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland |
title_full_unstemmed |
Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland |
title_sort |
metabolic profiling as a screening tool for cytotoxic compounds: identification of 3-alkyl pyridine alkaloids from sponges collected at a shallow water hydrothermal vent site north of iceland |
publisher |
MDPI AG |
publishDate |
2017 |
url |
https://hdl.handle.net/20.500.11815/340 https://doi.org/10.3390/md15020052 |
genre |
Iceland |
genre_facet |
Iceland |
op_relation |
Marine Drugs;15(3) Einarsdottir E, Magnusdottir M, Astarita G, Köck M, Ögmundsdottir HM, Thorsteinsdottir M, Rapp HT, Omarsdottir S, Paglia G. Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland. Marine Drugs. 2017; 15(2):52. doi:10.3390/md15020052 1660-3397 https://hdl.handle.net/20.500.11815/340 Marine Drugs doi:10.3390/md15020052 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/20.500.11815/34010.3390/md15020052 |
container_title |
Marine Drugs |
container_volume |
15 |
container_issue |
2 |
container_start_page |
52 |
_version_ |
1810451095251582976 |