Development of growth media from agricultural by-products for cultivation of PUFA-producing sicyoidochytrium minutum
Funding Information: This research was funded by: AVS-Fisheries research fund. Ministry of Fisheries and Agriculture in Iceland. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. The demand for novel sources of marine oils, which contain polyunsaturated fatty acids (PUFA...
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Online Access: | https://hdl.handle.net/20.500.11815/2868 https://doi.org/10.3390/md20010008 |
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ftopinvisindi:oai:opinvisindi.is:20.500.11815/2868 2023-11-12T04:19:34+01:00 Development of growth media from agricultural by-products for cultivation of PUFA-producing sicyoidochytrium minutum Eiríksdóttir, Heiðrún Stefánsson, Magnús Örn Einarsson, Hjörleifur School of Business and Science 2021 1263941 https://hdl.handle.net/20.500.11815/2868 https://doi.org/10.3390/md20010008 en eng Marine Drugs; 20(1) http://www.scopus.com/inward/record.url?scp=85121579117&partnerID=8YFLogxK Eiríksdóttir , H , Stefánsson , M Ö & Einarsson , H 2021 , ' Development of growth media from agricultural by-products for cultivation of PUFA-producing sicyoidochytrium minutum ' , Marine Drugs , vol. 20 , no. 1 , 8 . https://doi.org/10.3390/md20010008 1660-3397 45175993 ff395288-415d-4a49-99f5-a579808903ce 85121579117 https://hdl.handle.net/20.500.11815/2868 doi:10.3390/md20010008 info:eu-repo/semantics/openAccess Landbúnaðarafurðir Líffræði Fitusýrur DHA EPA Peptone Single-cell oil Thraustochytriaceae Drug Discovery /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article 2021 ftopinvisindi https://doi.org/20.500.11815/286810.3390/md20010008 2023-11-01T23:55:16Z Funding Information: This research was funded by: AVS-Fisheries research fund. Ministry of Fisheries and Agriculture in Iceland. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. The demand for novel sources of marine oils, which contain polyunsaturated fatty acids (PUFAs), has increased due to the realization of the importance of PUFAs, e.g., docosahexaenoic acid (DHA), in the human diet. However, the natural supply is limited. By-product peptones (BYPP) intended as a growth medium for the PUFA-producing strain Sicyoidochytrium minutum of family Thraustochytriaceae were produced after several experiments on the pancreatic digestion of bovine lungs and spleens. S. minutum was able to grow in a medium containing BYPP made from the pancreatic digestion of lung and spleen with glycerol, resulting in 1.14 ± 0.03 g cell dry weight (CDW)/L and 1.44 ± 0.24 g CDW/L, respectively, after 5 days of incubation at 25 °C, compared to 1.92 ± 0.25 g CDW/L in Basal Medium (BM) containing tryptone, peptone, and glycerol. The lipid content, obtained after growth in lung BYPP media with glycerol as a carbon source, was significantly higher (28.17% ± 1.33 of dry weight) than in the control basal medium (BM) (21.72% ± 2.45); however, DHA as a percentage of total fatty acids was lower in BYPP than in the control BM (25.24% ± 1.56 and 33.02% ± 2.37, respectively). It is concluded that low-value by-products from abattoirs can be used as ingredients for the cultivation of oligogenic Thraustochytriaceae. The demand for novel sources of marine oils, which contain polyunsaturated fatty acids (PUFAs), has increased due to the realization of the importance of PUFAs, e.g., docosahexaenoic acid (DHA), in the human diet. However, the natural supply is limited. By-product peptones (BYPP) intended as a growth medium for the PUFA-producing strain Sicyoidochytrium minutum of family Thraustochytriaceae were produced after several experiments on the pancreatic digestion of bovine lungs and spleens. S. minutum was able ... Article in Journal/Newspaper Iceland Opin vísindi (Iceland) Marine Drugs 20 1 8 |
institution |
Open Polar |
collection |
Opin vísindi (Iceland) |
op_collection_id |
ftopinvisindi |
language |
English |
topic |
Landbúnaðarafurðir Líffræði Fitusýrur DHA EPA Peptone Single-cell oil Thraustochytriaceae Drug Discovery |
spellingShingle |
Landbúnaðarafurðir Líffræði Fitusýrur DHA EPA Peptone Single-cell oil Thraustochytriaceae Drug Discovery Eiríksdóttir, Heiðrún Stefánsson, Magnús Örn Einarsson, Hjörleifur Development of growth media from agricultural by-products for cultivation of PUFA-producing sicyoidochytrium minutum |
topic_facet |
Landbúnaðarafurðir Líffræði Fitusýrur DHA EPA Peptone Single-cell oil Thraustochytriaceae Drug Discovery |
description |
Funding Information: This research was funded by: AVS-Fisheries research fund. Ministry of Fisheries and Agriculture in Iceland. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. The demand for novel sources of marine oils, which contain polyunsaturated fatty acids (PUFAs), has increased due to the realization of the importance of PUFAs, e.g., docosahexaenoic acid (DHA), in the human diet. However, the natural supply is limited. By-product peptones (BYPP) intended as a growth medium for the PUFA-producing strain Sicyoidochytrium minutum of family Thraustochytriaceae were produced after several experiments on the pancreatic digestion of bovine lungs and spleens. S. minutum was able to grow in a medium containing BYPP made from the pancreatic digestion of lung and spleen with glycerol, resulting in 1.14 ± 0.03 g cell dry weight (CDW)/L and 1.44 ± 0.24 g CDW/L, respectively, after 5 days of incubation at 25 °C, compared to 1.92 ± 0.25 g CDW/L in Basal Medium (BM) containing tryptone, peptone, and glycerol. The lipid content, obtained after growth in lung BYPP media with glycerol as a carbon source, was significantly higher (28.17% ± 1.33 of dry weight) than in the control basal medium (BM) (21.72% ± 2.45); however, DHA as a percentage of total fatty acids was lower in BYPP than in the control BM (25.24% ± 1.56 and 33.02% ± 2.37, respectively). It is concluded that low-value by-products from abattoirs can be used as ingredients for the cultivation of oligogenic Thraustochytriaceae. The demand for novel sources of marine oils, which contain polyunsaturated fatty acids (PUFAs), has increased due to the realization of the importance of PUFAs, e.g., docosahexaenoic acid (DHA), in the human diet. However, the natural supply is limited. By-product peptones (BYPP) intended as a growth medium for the PUFA-producing strain Sicyoidochytrium minutum of family Thraustochytriaceae were produced after several experiments on the pancreatic digestion of bovine lungs and spleens. S. minutum was able ... |
author2 |
School of Business and Science |
format |
Article in Journal/Newspaper |
author |
Eiríksdóttir, Heiðrún Stefánsson, Magnús Örn Einarsson, Hjörleifur |
author_facet |
Eiríksdóttir, Heiðrún Stefánsson, Magnús Örn Einarsson, Hjörleifur |
author_sort |
Eiríksdóttir, Heiðrún |
title |
Development of growth media from agricultural by-products for cultivation of PUFA-producing sicyoidochytrium minutum |
title_short |
Development of growth media from agricultural by-products for cultivation of PUFA-producing sicyoidochytrium minutum |
title_full |
Development of growth media from agricultural by-products for cultivation of PUFA-producing sicyoidochytrium minutum |
title_fullStr |
Development of growth media from agricultural by-products for cultivation of PUFA-producing sicyoidochytrium minutum |
title_full_unstemmed |
Development of growth media from agricultural by-products for cultivation of PUFA-producing sicyoidochytrium minutum |
title_sort |
development of growth media from agricultural by-products for cultivation of pufa-producing sicyoidochytrium minutum |
publishDate |
2021 |
url |
https://hdl.handle.net/20.500.11815/2868 https://doi.org/10.3390/md20010008 |
genre |
Iceland |
genre_facet |
Iceland |
op_relation |
Marine Drugs; 20(1) http://www.scopus.com/inward/record.url?scp=85121579117&partnerID=8YFLogxK Eiríksdóttir , H , Stefánsson , M Ö & Einarsson , H 2021 , ' Development of growth media from agricultural by-products for cultivation of PUFA-producing sicyoidochytrium minutum ' , Marine Drugs , vol. 20 , no. 1 , 8 . https://doi.org/10.3390/md20010008 1660-3397 45175993 ff395288-415d-4a49-99f5-a579808903ce 85121579117 https://hdl.handle.net/20.500.11815/2868 doi:10.3390/md20010008 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/20.500.11815/286810.3390/md20010008 |
container_title |
Marine Drugs |
container_volume |
20 |
container_issue |
1 |
container_start_page |
8 |
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