Adjuvants Enhance the Induction of Germinal Center and Antibody Secreting Cells in Spleen and Their Persistence in Bone Marrow of Neonatal Mice
Publisher's version (útgefin grein). The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fimmu.2019.02214/full#supplementary-material Immaturity of the immune system contributes to poor vaccine responses in early life. Germinal center...
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Online Access: | https://hdl.handle.net/20.500.11815/1643 https://doi.org/10.3389/fimmu.2019.02214 |
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ftopinvisindi:oai:opinvisindi.is:20.500.11815/1643 2023-05-15T16:49:37+02:00 Adjuvants Enhance the Induction of Germinal Center and Antibody Secreting Cells in Spleen and Their Persistence in Bone Marrow of Neonatal Mice Aradóttir Pind, Auður Anna Dubik, Magdalena Þórsdóttir, Sigrún Meinke, Andreas Harandi, Ali M. Holmgren, Jan Del Giudice, Giuseppe Jonsdottir, Ingileif Bjarnarson, Stefanía P Læknadeild (HÍ) Faculty of Medicine (UI) Heilbrigðisvísindasvið (HÍ) School of Health Sciences (UI) Háskóli Íslands University of Iceland 2019-09-26 2214 https://hdl.handle.net/20.500.11815/1643 https://doi.org/10.3389/fimmu.2019.02214 en eng Frontiers Media SA Frontiers in Immunology;10 https://www.frontiersin.org/article/10.3389/fimmu.2019.02214/full Aradottir Pind AA, Dubik M, Thorsdottir S, Meinke A, Harandi AM, Holmgren J, Del Giudice G, Jonsdottir I and Bjarnarson SP (2019) Adjuvants Enhance the Induction of Germinal Center and Antibody Secreting Cells in Spleen and Their Persistence in Bone Marrow of Neonatal Mice. Frontiers in Immunology 10:2214. doi:10.3389/fimmu.2019.02214 1664-3224 https://hdl.handle.net/20.500.11815/1643 Frontiers in Immunology doi:10.3389/fimmu.2019.02214 info:eu-repo/semantics/openAccess Adjuvant Antibody-secreting cell persistence Bone marrow Germinal center Neonate Protective antibodies Spleen Vaccination Bólusetningar Ónæmisfræði Milta Mótefni Beinmergur info:eu-repo/semantics/article 2019 ftopinvisindi https://doi.org/20.500.11815/1643 https://doi.org/10.3389/fimmu.2019.02214 2022-11-18T06:51:52Z Publisher's version (útgefin grein). The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fimmu.2019.02214/full#supplementary-material Immaturity of the immune system contributes to poor vaccine responses in early life. Germinal center (GC) activation is limited due to poorly developed follicular dendritic cells (FDC), causing generation of few antibody-secreting cells (ASCs) with limited survival and transient antibody responses. Herein, we compared the potential of five adjuvants, namely LT-K63, mmCT, MF59, IC31, and alum to overcome limitations of the neonatal immune system and to enhance and prolong responses of neonatal mice to a pneumococcal conjugate vaccine Pnc1-TT. The adjuvants LT-K63, mmCT, MF59, and IC31 significantly enhanced GC formation and FDC maturation in neonatal mice when co-administered with Pnc1-TT. This enhanced GC induction correlated with significantly enhanced vaccine-specific ASCs by LT-K63, mmCT, and MF59 in spleen 14 days after immunization. Furthermore, mmCT, MF59, and IC31 prolonged the induction of vaccine-specific ASCs in spleen and increased their persistence in bone marrow up to 9 weeks after immunization, as previously shown for LT-K63. Accordingly, serum Abs persisted above protective levels against pneumococcal bacteremia and pneumonia. In contrast, alum only enhanced the primary induction of vaccine-specific IgG Abs, which was transient. Our comparative study demonstrated that, in contrast to alum, LT-K63, mmCT, MF59, and IC31 can overcome limitations of the neonatal immune system and enhance both induction and persistence of protective immune response when administered with Pnc1-TT. These adjuvants are promising candidates for early life vaccination. AA was a recipient of a doctoral study grant from the University of Iceland Research Fund (2015-18). This study was financially supported by grants from the Icelandic Research Fund (130675051-53), The University of Iceland Research Fund (2014-17) and the ... Article in Journal/Newspaper Iceland Opin vísindi (Iceland) Frontiers in Immunology 10 |
institution |
Open Polar |
collection |
Opin vísindi (Iceland) |
op_collection_id |
ftopinvisindi |
language |
English |
topic |
Adjuvant Antibody-secreting cell persistence Bone marrow Germinal center Neonate Protective antibodies Spleen Vaccination Bólusetningar Ónæmisfræði Milta Mótefni Beinmergur |
spellingShingle |
Adjuvant Antibody-secreting cell persistence Bone marrow Germinal center Neonate Protective antibodies Spleen Vaccination Bólusetningar Ónæmisfræði Milta Mótefni Beinmergur Aradóttir Pind, Auður Anna Dubik, Magdalena Þórsdóttir, Sigrún Meinke, Andreas Harandi, Ali M. Holmgren, Jan Del Giudice, Giuseppe Jonsdottir, Ingileif Bjarnarson, Stefanía P Adjuvants Enhance the Induction of Germinal Center and Antibody Secreting Cells in Spleen and Their Persistence in Bone Marrow of Neonatal Mice |
topic_facet |
Adjuvant Antibody-secreting cell persistence Bone marrow Germinal center Neonate Protective antibodies Spleen Vaccination Bólusetningar Ónæmisfræði Milta Mótefni Beinmergur |
description |
Publisher's version (útgefin grein). The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fimmu.2019.02214/full#supplementary-material Immaturity of the immune system contributes to poor vaccine responses in early life. Germinal center (GC) activation is limited due to poorly developed follicular dendritic cells (FDC), causing generation of few antibody-secreting cells (ASCs) with limited survival and transient antibody responses. Herein, we compared the potential of five adjuvants, namely LT-K63, mmCT, MF59, IC31, and alum to overcome limitations of the neonatal immune system and to enhance and prolong responses of neonatal mice to a pneumococcal conjugate vaccine Pnc1-TT. The adjuvants LT-K63, mmCT, MF59, and IC31 significantly enhanced GC formation and FDC maturation in neonatal mice when co-administered with Pnc1-TT. This enhanced GC induction correlated with significantly enhanced vaccine-specific ASCs by LT-K63, mmCT, and MF59 in spleen 14 days after immunization. Furthermore, mmCT, MF59, and IC31 prolonged the induction of vaccine-specific ASCs in spleen and increased their persistence in bone marrow up to 9 weeks after immunization, as previously shown for LT-K63. Accordingly, serum Abs persisted above protective levels against pneumococcal bacteremia and pneumonia. In contrast, alum only enhanced the primary induction of vaccine-specific IgG Abs, which was transient. Our comparative study demonstrated that, in contrast to alum, LT-K63, mmCT, MF59, and IC31 can overcome limitations of the neonatal immune system and enhance both induction and persistence of protective immune response when administered with Pnc1-TT. These adjuvants are promising candidates for early life vaccination. AA was a recipient of a doctoral study grant from the University of Iceland Research Fund (2015-18). This study was financially supported by grants from the Icelandic Research Fund (130675051-53), The University of Iceland Research Fund (2014-17) and the ... |
author2 |
Læknadeild (HÍ) Faculty of Medicine (UI) Heilbrigðisvísindasvið (HÍ) School of Health Sciences (UI) Háskóli Íslands University of Iceland |
format |
Article in Journal/Newspaper |
author |
Aradóttir Pind, Auður Anna Dubik, Magdalena Þórsdóttir, Sigrún Meinke, Andreas Harandi, Ali M. Holmgren, Jan Del Giudice, Giuseppe Jonsdottir, Ingileif Bjarnarson, Stefanía P |
author_facet |
Aradóttir Pind, Auður Anna Dubik, Magdalena Þórsdóttir, Sigrún Meinke, Andreas Harandi, Ali M. Holmgren, Jan Del Giudice, Giuseppe Jonsdottir, Ingileif Bjarnarson, Stefanía P |
author_sort |
Aradóttir Pind, Auður Anna |
title |
Adjuvants Enhance the Induction of Germinal Center and Antibody Secreting Cells in Spleen and Their Persistence in Bone Marrow of Neonatal Mice |
title_short |
Adjuvants Enhance the Induction of Germinal Center and Antibody Secreting Cells in Spleen and Their Persistence in Bone Marrow of Neonatal Mice |
title_full |
Adjuvants Enhance the Induction of Germinal Center and Antibody Secreting Cells in Spleen and Their Persistence in Bone Marrow of Neonatal Mice |
title_fullStr |
Adjuvants Enhance the Induction of Germinal Center and Antibody Secreting Cells in Spleen and Their Persistence in Bone Marrow of Neonatal Mice |
title_full_unstemmed |
Adjuvants Enhance the Induction of Germinal Center and Antibody Secreting Cells in Spleen and Their Persistence in Bone Marrow of Neonatal Mice |
title_sort |
adjuvants enhance the induction of germinal center and antibody secreting cells in spleen and their persistence in bone marrow of neonatal mice |
publisher |
Frontiers Media SA |
publishDate |
2019 |
url |
https://hdl.handle.net/20.500.11815/1643 https://doi.org/10.3389/fimmu.2019.02214 |
genre |
Iceland |
genre_facet |
Iceland |
op_relation |
Frontiers in Immunology;10 https://www.frontiersin.org/article/10.3389/fimmu.2019.02214/full Aradottir Pind AA, Dubik M, Thorsdottir S, Meinke A, Harandi AM, Holmgren J, Del Giudice G, Jonsdottir I and Bjarnarson SP (2019) Adjuvants Enhance the Induction of Germinal Center and Antibody Secreting Cells in Spleen and Their Persistence in Bone Marrow of Neonatal Mice. Frontiers in Immunology 10:2214. doi:10.3389/fimmu.2019.02214 1664-3224 https://hdl.handle.net/20.500.11815/1643 Frontiers in Immunology doi:10.3389/fimmu.2019.02214 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/20.500.11815/1643 https://doi.org/10.3389/fimmu.2019.02214 |
container_title |
Frontiers in Immunology |
container_volume |
10 |
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1766039744525893632 |