Pentraxins CRP-I and CRP-II are post-translationally deiminated and differ in tissue specificity in cod (Gadus morhua L.) ontogeny
Publisher's version (útgefin grein) Pentraxins arefluid phase pattern recognition molecules that form an important part of the innate immunedefence and are conserved betweenfish and human. In Atlantic cod (Gadus morhuaL.), two pentraxin-likeproteins have been described, CRP-I and CRP-II. Here w...
Published in: | Developmental & Comparative Immunology |
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Format: | Article in Journal/Newspaper |
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Online Access: | https://hdl.handle.net/20.500.11815/1410 https://doi.org/10.1016/j.dci.2018.05.014 |
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ftopinvisindi:oai:opinvisindi.is:20.500.11815/1410 2023-05-15T15:27:47+02:00 Pentraxins CRP-I and CRP-II are post-translationally deiminated and differ in tissue specificity in cod (Gadus morhua L.) ontogeny Magnadottir, Bergljot Hayes, Polly Gísladóttir, Berglind Bragason, Birkir Hristova, Mariya Nicholas, Anthony P. Guðmundsdóttir, Sigríður Lange, Sigrun Tilraunastöð í meinafræði að Keldum (HÍ) Institute for Experimental Pathology, Keldur (UI) Háskóli Íslands (HÍ) University of Iceland (UI) 2018-10 1-11 https://hdl.handle.net/20.500.11815/1410 https://doi.org/10.1016/j.dci.2018.05.014 en eng Elsevier BV Developmental and Comparative Immunology;87 Magnadóttir, B., Hayes, P., Gísladóttir, B., Bragason, B. Þ., Hristova, M., Nicholas, A. P., . . . Lange, S. (2018). Pentraxins CRP-I and CRP-II are post-translationally deiminated and differ in tissue specificity in cod (Gadus morhua L.) ontogeny. Developmental & Comparative Immunology, 87, 1-11. doi:https://doi.org/10.1016/j.dci.2018.05.014 0145-305X https://hdl.handle.net/20.500.11815/1410 Developmental & Comparative Immunology doi:10.1016/j.dci.2018.05.014 info:eu-repo/semantics/openAccess Immunology Developmental Biology Pentraxin (CRP SAP) Protein deimination Mucosal immunity Amyloid Autoimmunity Cod (Gadus morhuaL.) Ontogeny Ónæmisfræði Þroskunarfræði Þorskur Prótín ÓNÆMI info:eu-repo/semantics/article 2018 ftopinvisindi https://doi.org/20.500.11815/1410 https://doi.org/10.1016/j.dci.2018.05.014 2022-11-18T06:51:48Z Publisher's version (útgefin grein) Pentraxins arefluid phase pattern recognition molecules that form an important part of the innate immunedefence and are conserved betweenfish and human. In Atlantic cod (Gadus morhuaL.), two pentraxin-likeproteins have been described, CRP-I and CRP-II. Here we show for thefirst time that these two CRP forms arepost-translationally deiminated (an irreversible conversion of arginine to citrulline) and differ with respect totissue specific localisation in cod ontogeny from 3 to 84 days post hatching. While both forms are expressed inliver, albeit at temporally differing levels, CRP-I shows a strong association with nervous tissue while CRP-II isstrongly associated to mucosal tissues of gut and skin. This indicates differing roles for the two pentraxin types inimmune responses and tissue remodelling, also elucidating novel roles for CRP-I in the nervous system. Thepresence of deimination positive bands for cod CRPs varied somewhat between mucus and serum, possiblyfacilitating CRP protein moonlighting, allowing the same protein to exhibit a range of biological functions andthus meeting different functional requirements in different tissues. The presentedfindings may further currentunderstanding of the diverse roles of pentraxins in teleost immune defences and tissue remodelling, as well as invarious human pathologies, including autoimmune diseases, amyloidosis and cancer. Thanks are due to Matthías Oddgeirsson, Agnar Steinarsson and other staff the staff at the Marine Institute's Mariculture Laboratory, Staður Grindavík, Iceland for providing sampling facilities and the fish. The authors also thank Margrét Jónsdóttir, Keldur, Institute for Experimental Pathology University of Iceland, for cod larvae sample preparation. This work was partly supported by The Icelandic Research Council (RANNIS), EC grant Fishaid QLK2-CT-2000-01076 and a University of Westminster start-up grant to SL. The authors declare no competing interest. Peer Reviewed Article in Journal/Newspaper atlantic cod Gadus morhua Grindavík Iceland Opin vísindi (Iceland) Grindavík ENVELOPE(-22.439,-22.439,63.838,63.838) Staður ENVELOPE(-22.367,-22.367,65.483,65.483) Westminster ENVELOPE(169.367,169.367,-84.983,-84.983) Developmental & Comparative Immunology 87 1 11 |
institution |
Open Polar |
collection |
Opin vísindi (Iceland) |
op_collection_id |
ftopinvisindi |
language |
English |
topic |
Immunology Developmental Biology Pentraxin (CRP SAP) Protein deimination Mucosal immunity Amyloid Autoimmunity Cod (Gadus morhuaL.) Ontogeny Ónæmisfræði Þroskunarfræði Þorskur Prótín ÓNÆMI |
spellingShingle |
Immunology Developmental Biology Pentraxin (CRP SAP) Protein deimination Mucosal immunity Amyloid Autoimmunity Cod (Gadus morhuaL.) Ontogeny Ónæmisfræði Þroskunarfræði Þorskur Prótín ÓNÆMI Magnadottir, Bergljot Hayes, Polly Gísladóttir, Berglind Bragason, Birkir Hristova, Mariya Nicholas, Anthony P. Guðmundsdóttir, Sigríður Lange, Sigrun Pentraxins CRP-I and CRP-II are post-translationally deiminated and differ in tissue specificity in cod (Gadus morhua L.) ontogeny |
topic_facet |
Immunology Developmental Biology Pentraxin (CRP SAP) Protein deimination Mucosal immunity Amyloid Autoimmunity Cod (Gadus morhuaL.) Ontogeny Ónæmisfræði Þroskunarfræði Þorskur Prótín ÓNÆMI |
description |
Publisher's version (útgefin grein) Pentraxins arefluid phase pattern recognition molecules that form an important part of the innate immunedefence and are conserved betweenfish and human. In Atlantic cod (Gadus morhuaL.), two pentraxin-likeproteins have been described, CRP-I and CRP-II. Here we show for thefirst time that these two CRP forms arepost-translationally deiminated (an irreversible conversion of arginine to citrulline) and differ with respect totissue specific localisation in cod ontogeny from 3 to 84 days post hatching. While both forms are expressed inliver, albeit at temporally differing levels, CRP-I shows a strong association with nervous tissue while CRP-II isstrongly associated to mucosal tissues of gut and skin. This indicates differing roles for the two pentraxin types inimmune responses and tissue remodelling, also elucidating novel roles for CRP-I in the nervous system. Thepresence of deimination positive bands for cod CRPs varied somewhat between mucus and serum, possiblyfacilitating CRP protein moonlighting, allowing the same protein to exhibit a range of biological functions andthus meeting different functional requirements in different tissues. The presentedfindings may further currentunderstanding of the diverse roles of pentraxins in teleost immune defences and tissue remodelling, as well as invarious human pathologies, including autoimmune diseases, amyloidosis and cancer. Thanks are due to Matthías Oddgeirsson, Agnar Steinarsson and other staff the staff at the Marine Institute's Mariculture Laboratory, Staður Grindavík, Iceland for providing sampling facilities and the fish. The authors also thank Margrét Jónsdóttir, Keldur, Institute for Experimental Pathology University of Iceland, for cod larvae sample preparation. This work was partly supported by The Icelandic Research Council (RANNIS), EC grant Fishaid QLK2-CT-2000-01076 and a University of Westminster start-up grant to SL. The authors declare no competing interest. Peer Reviewed |
author2 |
Tilraunastöð í meinafræði að Keldum (HÍ) Institute for Experimental Pathology, Keldur (UI) Háskóli Íslands (HÍ) University of Iceland (UI) |
format |
Article in Journal/Newspaper |
author |
Magnadottir, Bergljot Hayes, Polly Gísladóttir, Berglind Bragason, Birkir Hristova, Mariya Nicholas, Anthony P. Guðmundsdóttir, Sigríður Lange, Sigrun |
author_facet |
Magnadottir, Bergljot Hayes, Polly Gísladóttir, Berglind Bragason, Birkir Hristova, Mariya Nicholas, Anthony P. Guðmundsdóttir, Sigríður Lange, Sigrun |
author_sort |
Magnadottir, Bergljot |
title |
Pentraxins CRP-I and CRP-II are post-translationally deiminated and differ in tissue specificity in cod (Gadus morhua L.) ontogeny |
title_short |
Pentraxins CRP-I and CRP-II are post-translationally deiminated and differ in tissue specificity in cod (Gadus morhua L.) ontogeny |
title_full |
Pentraxins CRP-I and CRP-II are post-translationally deiminated and differ in tissue specificity in cod (Gadus morhua L.) ontogeny |
title_fullStr |
Pentraxins CRP-I and CRP-II are post-translationally deiminated and differ in tissue specificity in cod (Gadus morhua L.) ontogeny |
title_full_unstemmed |
Pentraxins CRP-I and CRP-II are post-translationally deiminated and differ in tissue specificity in cod (Gadus morhua L.) ontogeny |
title_sort |
pentraxins crp-i and crp-ii are post-translationally deiminated and differ in tissue specificity in cod (gadus morhua l.) ontogeny |
publisher |
Elsevier BV |
publishDate |
2018 |
url |
https://hdl.handle.net/20.500.11815/1410 https://doi.org/10.1016/j.dci.2018.05.014 |
long_lat |
ENVELOPE(-22.439,-22.439,63.838,63.838) ENVELOPE(-22.367,-22.367,65.483,65.483) ENVELOPE(169.367,169.367,-84.983,-84.983) |
geographic |
Grindavík Staður Westminster |
geographic_facet |
Grindavík Staður Westminster |
genre |
atlantic cod Gadus morhua Grindavík Iceland |
genre_facet |
atlantic cod Gadus morhua Grindavík Iceland |
op_relation |
Developmental and Comparative Immunology;87 Magnadóttir, B., Hayes, P., Gísladóttir, B., Bragason, B. Þ., Hristova, M., Nicholas, A. P., . . . Lange, S. (2018). Pentraxins CRP-I and CRP-II are post-translationally deiminated and differ in tissue specificity in cod (Gadus morhua L.) ontogeny. Developmental & Comparative Immunology, 87, 1-11. doi:https://doi.org/10.1016/j.dci.2018.05.014 0145-305X https://hdl.handle.net/20.500.11815/1410 Developmental & Comparative Immunology doi:10.1016/j.dci.2018.05.014 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/20.500.11815/1410 https://doi.org/10.1016/j.dci.2018.05.014 |
container_title |
Developmental & Comparative Immunology |
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87 |
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1 |
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11 |
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1766358190744666112 |