Outbreak of viral haemorrhagic septicaemia (VHS) in lumpfish (Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV
Publisher's version (útgefin grein) A novel viral haemorrhagic septicaemia virus (VHSV) of genotype IV was isolated from wild lumpfish (Cyclopterus lumpus), brought to a land-based farm in Iceland, to serve as broodfish. Two groups of lumpfish juveniles, kept in tanks in the same facility, got...
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Online Access: | https://hdl.handle.net/20.500.11815/1336 https://doi.org/10.1111/jfd.12910 |
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ftopinvisindi:oai:opinvisindi.is:20.500.11815/1336 2023-05-15T15:30:50+02:00 Outbreak of viral haemorrhagic septicaemia (VHS) in lumpfish (Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV Guðmundsdóttir, Sigríður Vendramin, Niccoló Cuenca, Argelia Sigurðardóttir, Heiða Kristmundsson, Árni Iburg, Tine Moesgaard Olesen, Niels Jørgen Tilraunastöð í meinafræði að Keldum (HÍ) Institute for Experimental Pathology, Keldur (UI) Háskóli Íslands University of Iceland 2018-11-05 47-62 https://hdl.handle.net/20.500.11815/1336 https://doi.org/10.1111/jfd.12910 en eng Wiley info:eu-repo/grantAgreement/EC/H2020/634429 Journal of Fish Diseases;42(1) Guðmundsdóttir, S, Vendramin, N, Cuenca, A, et al. Outbreak of viral haemorrhagic septicaemia (VHS) in lumpfish (Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV. J Fish Dis. 2019; 42: 47– 62. https://doi.org/10.1111/jfd.12910 0140-7775 1365-2761 (eISSN) https://hdl.handle.net/20.500.11815/1336 Journal of Fish Diseases doi:10.1111/jfd.12910 info:eu-repo/semantics/openAccess Challenge models Genotype IV Iceland Lumpfish Viral haemorrhagic septicaemia virus (VHSV) Hrognkelsi Veirur info:eu-repo/semantics/article 2018 ftopinvisindi https://doi.org/20.500.11815/1336 https://doi.org/10.1111/jfd.12910 2022-11-18T06:51:46Z Publisher's version (útgefin grein) A novel viral haemorrhagic septicaemia virus (VHSV) of genotype IV was isolated from wild lumpfish (Cyclopterus lumpus), brought to a land-based farm in Iceland, to serve as broodfish. Two groups of lumpfish juveniles, kept in tanks in the same facility, got infected. The virus isolated was identified as VHSV by ELISA and real-time RT-PCR. Phylogenetic analysis, based on the glycoprotein (G) gene sequences, may indicate a novel subgroup of VHSV genotype IV. In controlled laboratory exposure studies with this new isolate, there was 3% survival in the I.P. injection challenged group while there was 90% survival in the immersion group. VHSV was not re-isolated from fish challenged by immersion. In a cohabitation trial, lumpfish infected I.P. (shedders) were placed in tanks with naïve lumpfish as well as naïve Atlantic salmon (Salmo salar L.). 10% of the lumpfish shedders and 43%–50% of the cohabiting lumpfish survived after 4 weeks. 80%–92% of the Atlantic salmon survived, but no viral RNA was detected by real-time RT-PCR nor VHSV was isolated from Atlantic salmon. This is the first isolation of a notifiable virus in Iceland and the first report of VHSV of genotype IV in European waters. H2020 SFS-2014-2 ParaFishControl, Grant/ Award Number: 634429; European Union Reference Laboratory for Fish Diseases Grant Decision SI2.725290 Peer Reviewed Article in Journal/Newspaper Atlantic salmon Iceland Salmo salar Opin vísindi (Iceland) Journal of Fish Diseases 42 1 47 62 |
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Open Polar |
collection |
Opin vísindi (Iceland) |
op_collection_id |
ftopinvisindi |
language |
English |
topic |
Challenge models Genotype IV Iceland Lumpfish Viral haemorrhagic septicaemia virus (VHSV) Hrognkelsi Veirur |
spellingShingle |
Challenge models Genotype IV Iceland Lumpfish Viral haemorrhagic septicaemia virus (VHSV) Hrognkelsi Veirur Guðmundsdóttir, Sigríður Vendramin, Niccoló Cuenca, Argelia Sigurðardóttir, Heiða Kristmundsson, Árni Iburg, Tine Moesgaard Olesen, Niels Jørgen Outbreak of viral haemorrhagic septicaemia (VHS) in lumpfish (Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV |
topic_facet |
Challenge models Genotype IV Iceland Lumpfish Viral haemorrhagic septicaemia virus (VHSV) Hrognkelsi Veirur |
description |
Publisher's version (útgefin grein) A novel viral haemorrhagic septicaemia virus (VHSV) of genotype IV was isolated from wild lumpfish (Cyclopterus lumpus), brought to a land-based farm in Iceland, to serve as broodfish. Two groups of lumpfish juveniles, kept in tanks in the same facility, got infected. The virus isolated was identified as VHSV by ELISA and real-time RT-PCR. Phylogenetic analysis, based on the glycoprotein (G) gene sequences, may indicate a novel subgroup of VHSV genotype IV. In controlled laboratory exposure studies with this new isolate, there was 3% survival in the I.P. injection challenged group while there was 90% survival in the immersion group. VHSV was not re-isolated from fish challenged by immersion. In a cohabitation trial, lumpfish infected I.P. (shedders) were placed in tanks with naïve lumpfish as well as naïve Atlantic salmon (Salmo salar L.). 10% of the lumpfish shedders and 43%–50% of the cohabiting lumpfish survived after 4 weeks. 80%–92% of the Atlantic salmon survived, but no viral RNA was detected by real-time RT-PCR nor VHSV was isolated from Atlantic salmon. This is the first isolation of a notifiable virus in Iceland and the first report of VHSV of genotype IV in European waters. H2020 SFS-2014-2 ParaFishControl, Grant/ Award Number: 634429; European Union Reference Laboratory for Fish Diseases Grant Decision SI2.725290 Peer Reviewed |
author2 |
Tilraunastöð í meinafræði að Keldum (HÍ) Institute for Experimental Pathology, Keldur (UI) Háskóli Íslands University of Iceland |
format |
Article in Journal/Newspaper |
author |
Guðmundsdóttir, Sigríður Vendramin, Niccoló Cuenca, Argelia Sigurðardóttir, Heiða Kristmundsson, Árni Iburg, Tine Moesgaard Olesen, Niels Jørgen |
author_facet |
Guðmundsdóttir, Sigríður Vendramin, Niccoló Cuenca, Argelia Sigurðardóttir, Heiða Kristmundsson, Árni Iburg, Tine Moesgaard Olesen, Niels Jørgen |
author_sort |
Guðmundsdóttir, Sigríður |
title |
Outbreak of viral haemorrhagic septicaemia (VHS) in lumpfish (Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV |
title_short |
Outbreak of viral haemorrhagic septicaemia (VHS) in lumpfish (Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV |
title_full |
Outbreak of viral haemorrhagic septicaemia (VHS) in lumpfish (Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV |
title_fullStr |
Outbreak of viral haemorrhagic septicaemia (VHS) in lumpfish (Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV |
title_full_unstemmed |
Outbreak of viral haemorrhagic septicaemia (VHS) in lumpfish (Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV |
title_sort |
outbreak of viral haemorrhagic septicaemia (vhs) in lumpfish (cyclopterus lumpus) in iceland caused by vhs virus genotype iv |
publisher |
Wiley |
publishDate |
2018 |
url |
https://hdl.handle.net/20.500.11815/1336 https://doi.org/10.1111/jfd.12910 |
genre |
Atlantic salmon Iceland Salmo salar |
genre_facet |
Atlantic salmon Iceland Salmo salar |
op_relation |
info:eu-repo/grantAgreement/EC/H2020/634429 Journal of Fish Diseases;42(1) Guðmundsdóttir, S, Vendramin, N, Cuenca, A, et al. Outbreak of viral haemorrhagic septicaemia (VHS) in lumpfish (Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV. J Fish Dis. 2019; 42: 47– 62. https://doi.org/10.1111/jfd.12910 0140-7775 1365-2761 (eISSN) https://hdl.handle.net/20.500.11815/1336 Journal of Fish Diseases doi:10.1111/jfd.12910 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/20.500.11815/1336 https://doi.org/10.1111/jfd.12910 |
container_title |
Journal of Fish Diseases |
container_volume |
42 |
container_issue |
1 |
container_start_page |
47 |
op_container_end_page |
62 |
_version_ |
1766361301376827392 |