Arctic microbes – The phylogenetic and functional diversity of prokaryotes at Colour Peak
The aquifer fed springs of Colour Peak in the Canadian arctic are perennially cold, highly saline and sulphur rich. These properties have led to the springs being considered analogous to subsurface aqueous environments on Mars[1-3]. Therefore, understanding the phylogenetic and functional diversity...
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ftopenunivgb:oai:oro.open.ac.uk:62326 2023-06-11T04:07:43+02:00 Arctic microbes – The phylogenetic and functional diversity of prokaryotes at Colour Peak Macey, Michael Christopher Fox-Powell, Mark Stephens, Ben Schwenzer, Susanne P. Pearson, Victoria K. Cousins, Claire R. Olsson-Francis, Karen 2018 https://oro.open.ac.uk/62326/ http://venueswales.com/mmeg2018/ unknown Macey, Michael Christopher <http://oro.open.ac.uk/view/person/mm34528.html>; Fox-Powell, Mark; Stephens, Ben <http://oro.open.ac.uk/view/person/bs5968.html>; Schwenzer, Susanne P. <http://oro.open.ac.uk/view/person/ss24846.html>; Pearson, Victoria K. <http://oro.open.ac.uk/view/person/vkp23.html>; Cousins, Claire R. and Olsson-Francis, Karen <http://oro.open.ac.uk/view/person/ko627.html> (2018). Arctic microbes – The phylogenetic and functional diversity of prokaryotes at Colour Peak. In: Molecular Microbial Ecology Group (MMEG) Meeting 2018, 17-19 Dec 2018, Swansea. Conference or Workshop Item None PeerReviewed 2018 ftopenunivgb 2023-05-28T06:00:17Z The aquifer fed springs of Colour Peak in the Canadian arctic are perennially cold, highly saline and sulphur rich. These properties have led to the springs being considered analogous to subsurface aqueous environments on Mars[1-3]. Therefore, understanding the phylogenetic and functional diversity of prokaryotes that are capable of growing in this environment is important for understanding the mechanisms conducive to life within these extra-terrestrial environments. Cultivation-dependant and -independent techniques were used to assess the diversity of prokaryotes present in sediment collected from a hyper-saline cold pool at Colour Peak. The microbial community that is present and active at the site was characterised through the sequencing of 16S rRNA gene amplicons amplified following the extraction of DNA and RNA from the sediment. To further investigate the metabolic profile of the prokaryotes within the site, the presence and expression of functional genes relating to the cycling of carbon, nitrogen and sulphur compounds was also assayed using PCR. The results gained from the cultivation-independent work was used to inform enrichment strategies, allowing for the isolation and characterisation of several halophilic isolates. Through assessment of the physiologies of the isolates it was possible to develop a further insight into the metabolic potential underpinning survival in Colour Peak. Through this work we have been able to develop an indication as to identity of the key players and metabolic guilds within the microbial community of this site and further our understanding of survival under this range of selection pressures. Conference Object Arctic Arctic microbes Arctic The Open University: Open Research Online (ORO) Arctic Colour Peak ENVELOPE(-91.284,-91.284,79.469,79.469) |
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Open Polar |
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The Open University: Open Research Online (ORO) |
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ftopenunivgb |
language |
unknown |
description |
The aquifer fed springs of Colour Peak in the Canadian arctic are perennially cold, highly saline and sulphur rich. These properties have led to the springs being considered analogous to subsurface aqueous environments on Mars[1-3]. Therefore, understanding the phylogenetic and functional diversity of prokaryotes that are capable of growing in this environment is important for understanding the mechanisms conducive to life within these extra-terrestrial environments. Cultivation-dependant and -independent techniques were used to assess the diversity of prokaryotes present in sediment collected from a hyper-saline cold pool at Colour Peak. The microbial community that is present and active at the site was characterised through the sequencing of 16S rRNA gene amplicons amplified following the extraction of DNA and RNA from the sediment. To further investigate the metabolic profile of the prokaryotes within the site, the presence and expression of functional genes relating to the cycling of carbon, nitrogen and sulphur compounds was also assayed using PCR. The results gained from the cultivation-independent work was used to inform enrichment strategies, allowing for the isolation and characterisation of several halophilic isolates. Through assessment of the physiologies of the isolates it was possible to develop a further insight into the metabolic potential underpinning survival in Colour Peak. Through this work we have been able to develop an indication as to identity of the key players and metabolic guilds within the microbial community of this site and further our understanding of survival under this range of selection pressures. |
format |
Conference Object |
author |
Macey, Michael Christopher Fox-Powell, Mark Stephens, Ben Schwenzer, Susanne P. Pearson, Victoria K. Cousins, Claire R. Olsson-Francis, Karen |
spellingShingle |
Macey, Michael Christopher Fox-Powell, Mark Stephens, Ben Schwenzer, Susanne P. Pearson, Victoria K. Cousins, Claire R. Olsson-Francis, Karen Arctic microbes – The phylogenetic and functional diversity of prokaryotes at Colour Peak |
author_facet |
Macey, Michael Christopher Fox-Powell, Mark Stephens, Ben Schwenzer, Susanne P. Pearson, Victoria K. Cousins, Claire R. Olsson-Francis, Karen |
author_sort |
Macey, Michael Christopher |
title |
Arctic microbes – The phylogenetic and functional diversity of prokaryotes at Colour Peak |
title_short |
Arctic microbes – The phylogenetic and functional diversity of prokaryotes at Colour Peak |
title_full |
Arctic microbes – The phylogenetic and functional diversity of prokaryotes at Colour Peak |
title_fullStr |
Arctic microbes – The phylogenetic and functional diversity of prokaryotes at Colour Peak |
title_full_unstemmed |
Arctic microbes – The phylogenetic and functional diversity of prokaryotes at Colour Peak |
title_sort |
arctic microbes – the phylogenetic and functional diversity of prokaryotes at colour peak |
publishDate |
2018 |
url |
https://oro.open.ac.uk/62326/ http://venueswales.com/mmeg2018/ |
long_lat |
ENVELOPE(-91.284,-91.284,79.469,79.469) |
geographic |
Arctic Colour Peak |
geographic_facet |
Arctic Colour Peak |
genre |
Arctic Arctic microbes Arctic |
genre_facet |
Arctic Arctic microbes Arctic |
op_relation |
Macey, Michael Christopher <http://oro.open.ac.uk/view/person/mm34528.html>; Fox-Powell, Mark; Stephens, Ben <http://oro.open.ac.uk/view/person/bs5968.html>; Schwenzer, Susanne P. <http://oro.open.ac.uk/view/person/ss24846.html>; Pearson, Victoria K. <http://oro.open.ac.uk/view/person/vkp23.html>; Cousins, Claire R. and Olsson-Francis, Karen <http://oro.open.ac.uk/view/person/ko627.html> (2018). Arctic microbes – The phylogenetic and functional diversity of prokaryotes at Colour Peak. In: Molecular Microbial Ecology Group (MMEG) Meeting 2018, 17-19 Dec 2018, Swansea. |
_version_ |
1768380874668113920 |