Land coverage influences the bacterial community composition in the critical zone of a sub-Arctic basaltic environment
Silicate weathering improves soils by releasing bioessential nutrients from the bedrock to the soil ecosystem. However, whether bacteria are capable of inhabiting subsurface critical zones (zone of active rock weathering), and their role therein, are unknown. Next-generation sequencing and community...
Published in: | FEMS Microbiology Ecology |
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2013
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Online Access: | https://oro.open.ac.uk/41345/ https://oro.open.ac.uk/41345/1/Summers%202013.pdf https://doi.org/10.1111/1574-6941.12167 |
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ftopenunivgb:oai:oro.open.ac.uk:41345 2023-06-11T04:07:36+02:00 Land coverage influences the bacterial community composition in the critical zone of a sub-Arctic basaltic environment Summers, Stephen Whiteley, Andrew S. Kelly, Laura C. Cockell, Charles S. 2013-12 application/pdf https://oro.open.ac.uk/41345/ https://oro.open.ac.uk/41345/1/Summers%202013.pdf https://doi.org/10.1111/1574-6941.12167 unknown https://oro.open.ac.uk/41345/1/Summers%202013.pdf Summers, Stephen <http://oro.open.ac.uk/view/person/ss29877.html>; Whiteley, Andrew S.; Kelly, Laura C. and Cockell, Charles S. (2013). Land coverage influences the bacterial community composition in the critical zone of a sub-Arctic basaltic environment. FEMS Microbiology Ecology, 86(3) pp. 381–393. Journal Item OU Users Only PeerReviewed 2013 ftopenunivgb https://doi.org/10.1111/1574-6941.12167 2023-05-28T05:51:54Z Silicate weathering improves soils by releasing bioessential nutrients from the bedrock to the soil ecosystem. However, whether bacteria are capable of inhabiting subsurface critical zones (zone of active rock weathering), and their role therein, are unknown. Next-generation sequencing and community fingerprinting permitted us to characterize communities from an Icelandic critical zone environment. Communities were compared with respect to physico-chemical properties of the environment to determine the factors influencing bacterial diversity. We showed that land coverage influenced critical zone communities. Analysis of tree-covered site (TCS) soils exhibited high cell densities (TCS = 2.25 9 10 7 g -1 ), whereas lichen- and moss-covered sites (LMS) had lower cell densities (LMS = 1.06 X 10 7 cells g -1 ), thought to be a result of the organic carbon produced by the trees. Differences in the bacterial community were observed from the abundance of 16S rRNA gene sequences affiliated with Acidobacteria and Proteobacteria , with TCS possessing higher abundances of Proteobacteria [no of sequences: LMS = 1526 (±497); TCS = 2214 (±531)], specifically Alpha- and Betaproteobacteria , and lower Acidobacteria numbers [no of sequences: LMS = 1244 (±338); TCS = 598 (±140)]. Diversity indices and 16S rRNA gene rarefaction showed that communities from TCS soils had lower α-diversity than sites without, indicative of specialized communities at sites with root-forming plants. Article in Journal/Newspaper Arctic Arctic The Open University: Open Research Online (ORO) Arctic FEMS Microbiology Ecology 86 3 381 393 |
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Open Polar |
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The Open University: Open Research Online (ORO) |
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ftopenunivgb |
language |
unknown |
description |
Silicate weathering improves soils by releasing bioessential nutrients from the bedrock to the soil ecosystem. However, whether bacteria are capable of inhabiting subsurface critical zones (zone of active rock weathering), and their role therein, are unknown. Next-generation sequencing and community fingerprinting permitted us to characterize communities from an Icelandic critical zone environment. Communities were compared with respect to physico-chemical properties of the environment to determine the factors influencing bacterial diversity. We showed that land coverage influenced critical zone communities. Analysis of tree-covered site (TCS) soils exhibited high cell densities (TCS = 2.25 9 10 7 g -1 ), whereas lichen- and moss-covered sites (LMS) had lower cell densities (LMS = 1.06 X 10 7 cells g -1 ), thought to be a result of the organic carbon produced by the trees. Differences in the bacterial community were observed from the abundance of 16S rRNA gene sequences affiliated with Acidobacteria and Proteobacteria , with TCS possessing higher abundances of Proteobacteria [no of sequences: LMS = 1526 (±497); TCS = 2214 (±531)], specifically Alpha- and Betaproteobacteria , and lower Acidobacteria numbers [no of sequences: LMS = 1244 (±338); TCS = 598 (±140)]. Diversity indices and 16S rRNA gene rarefaction showed that communities from TCS soils had lower α-diversity than sites without, indicative of specialized communities at sites with root-forming plants. |
format |
Article in Journal/Newspaper |
author |
Summers, Stephen Whiteley, Andrew S. Kelly, Laura C. Cockell, Charles S. |
spellingShingle |
Summers, Stephen Whiteley, Andrew S. Kelly, Laura C. Cockell, Charles S. Land coverage influences the bacterial community composition in the critical zone of a sub-Arctic basaltic environment |
author_facet |
Summers, Stephen Whiteley, Andrew S. Kelly, Laura C. Cockell, Charles S. |
author_sort |
Summers, Stephen |
title |
Land coverage influences the bacterial community composition in the critical zone of a sub-Arctic basaltic environment |
title_short |
Land coverage influences the bacterial community composition in the critical zone of a sub-Arctic basaltic environment |
title_full |
Land coverage influences the bacterial community composition in the critical zone of a sub-Arctic basaltic environment |
title_fullStr |
Land coverage influences the bacterial community composition in the critical zone of a sub-Arctic basaltic environment |
title_full_unstemmed |
Land coverage influences the bacterial community composition in the critical zone of a sub-Arctic basaltic environment |
title_sort |
land coverage influences the bacterial community composition in the critical zone of a sub-arctic basaltic environment |
publishDate |
2013 |
url |
https://oro.open.ac.uk/41345/ https://oro.open.ac.uk/41345/1/Summers%202013.pdf https://doi.org/10.1111/1574-6941.12167 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Arctic |
genre_facet |
Arctic Arctic |
op_relation |
https://oro.open.ac.uk/41345/1/Summers%202013.pdf Summers, Stephen <http://oro.open.ac.uk/view/person/ss29877.html>; Whiteley, Andrew S.; Kelly, Laura C. and Cockell, Charles S. (2013). Land coverage influences the bacterial community composition in the critical zone of a sub-Arctic basaltic environment. FEMS Microbiology Ecology, 86(3) pp. 381–393. |
op_doi |
https://doi.org/10.1111/1574-6941.12167 |
container_title |
FEMS Microbiology Ecology |
container_volume |
86 |
container_issue |
3 |
container_start_page |
381 |
op_container_end_page |
393 |
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1768380780272156672 |