Solvent-Free Isoamyl Acetate Production via Enzymatic Esterification
Isoamyl acetate is an organic compound which is mainly used as flavor additive in food industries. Traditionally, the food flavor has been produced by extraction from plants, followed by chemical synthesis route which then shifted to biocatalytic route due to consumer’s awareness and inclination t...
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ftopenresearchl:oai:biblioboard.com:3fcda06b-9d2e-4825-8587-07677000b96a 2023-07-02T03:30:43+02:00 Solvent-Free Isoamyl Acetate Production via Enzymatic Esterification Abd Shukor, Syamsul Rizal Yusoff Azudin, Nurhazwani 2018-01-01T00:00:00Z application/pdf https://openresearchlibrary.org/viewer/3fcda06b-9d2e-4825-8587-07677000b96a https://openresearchlibrary.org/ext/api/media/3fcda06b-9d2e-4825-8587-07677000b96a/assets/external_content.pdf English eng https://openresearchlibrary.org/viewer/3fcda06b-9d2e-4825-8587-07677000b96a https://openresearchlibrary.org/ext/api/media/3fcda06b-9d2e-4825-8587-07677000b96a/assets/external_content.pdf https://creativecommons.org/licenses/by/4.0/legalcode MODID-6d55e02e354:IntechOpen Science / Life Sciences / Biochemistry bisacsh:SCI007000 CHAPTER 2018 ftopenresearchl 2023-06-11T22:29:59Z Isoamyl acetate is an organic compound which is mainly used as flavor additive in food industries. Traditionally, the food flavor has been produced by extraction from plants, followed by chemical synthesis route which then shifted to biocatalytic route due to consumer’s awareness and inclination toward natural products. This study was carried out to examine the reaction synthesis between acetic anhydride and isoamyl alcohol in the presence of Candida antarctica Lipase-B (CALB) as a catalyst in solvent-free system (SFS). Results show that two reactions took place between acetic anhydride and isoamyl alcohol. The effect of different reaction parameters on the final yield of isoamyl acetate and the optimization of process parameters using a statistical tool were also investigated with response surface methodology (RSM). It was found that the optimum isoamyl acetate yield is at reaction temperature 30°C, acid/alcohol molar ratio 0.10, and enzyme loading 4.14%. The regression coefficient for optimization based on RSM was 0.9961. Errors resulted from model validation is less than 1% and is acceptable for real-life application. RSM model and first principle model were selected to determine the reaction kinetics and yield of reaction for isoamyl acetate. The results showed that RSM model provides a good predication of the esterification system with R2 value of 0.90. Article in Journal/Newspaper Antarc* Antarctica Open Research Library |
institution |
Open Polar |
collection |
Open Research Library |
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ftopenresearchl |
language |
English |
topic |
Science / Life Sciences / Biochemistry bisacsh:SCI007000 |
spellingShingle |
Science / Life Sciences / Biochemistry bisacsh:SCI007000 Abd Shukor, Syamsul Rizal Solvent-Free Isoamyl Acetate Production via Enzymatic Esterification |
topic_facet |
Science / Life Sciences / Biochemistry bisacsh:SCI007000 |
description |
Isoamyl acetate is an organic compound which is mainly used as flavor additive in food industries. Traditionally, the food flavor has been produced by extraction from plants, followed by chemical synthesis route which then shifted to biocatalytic route due to consumer’s awareness and inclination toward natural products. This study was carried out to examine the reaction synthesis between acetic anhydride and isoamyl alcohol in the presence of Candida antarctica Lipase-B (CALB) as a catalyst in solvent-free system (SFS). Results show that two reactions took place between acetic anhydride and isoamyl alcohol. The effect of different reaction parameters on the final yield of isoamyl acetate and the optimization of process parameters using a statistical tool were also investigated with response surface methodology (RSM). It was found that the optimum isoamyl acetate yield is at reaction temperature 30°C, acid/alcohol molar ratio 0.10, and enzyme loading 4.14%. The regression coefficient for optimization based on RSM was 0.9961. Errors resulted from model validation is less than 1% and is acceptable for real-life application. RSM model and first principle model were selected to determine the reaction kinetics and yield of reaction for isoamyl acetate. The results showed that RSM model provides a good predication of the esterification system with R2 value of 0.90. |
author2 |
Yusoff Azudin, Nurhazwani |
format |
Article in Journal/Newspaper |
author |
Abd Shukor, Syamsul Rizal |
author_facet |
Abd Shukor, Syamsul Rizal |
author_sort |
Abd Shukor, Syamsul Rizal |
title |
Solvent-Free Isoamyl Acetate Production via Enzymatic Esterification |
title_short |
Solvent-Free Isoamyl Acetate Production via Enzymatic Esterification |
title_full |
Solvent-Free Isoamyl Acetate Production via Enzymatic Esterification |
title_fullStr |
Solvent-Free Isoamyl Acetate Production via Enzymatic Esterification |
title_full_unstemmed |
Solvent-Free Isoamyl Acetate Production via Enzymatic Esterification |
title_sort |
solvent-free isoamyl acetate production via enzymatic esterification |
publishDate |
2018 |
url |
https://openresearchlibrary.org/viewer/3fcda06b-9d2e-4825-8587-07677000b96a https://openresearchlibrary.org/ext/api/media/3fcda06b-9d2e-4825-8587-07677000b96a/assets/external_content.pdf |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
MODID-6d55e02e354:IntechOpen |
op_relation |
https://openresearchlibrary.org/viewer/3fcda06b-9d2e-4825-8587-07677000b96a https://openresearchlibrary.org/ext/api/media/3fcda06b-9d2e-4825-8587-07677000b96a/assets/external_content.pdf |
op_rights |
https://creativecommons.org/licenses/by/4.0/legalcode |
_version_ |
1770275003932082176 |