Genome sequence and functional genomic analysis of the oil-degrading bacteriumOleispira antarctica
Ubiquitous bacteria from the genus Oleispira drive oil degradation in the largest environment on Earth, the cold and deep sea. Here we report the genome sequence of Oleispira antarctica and show that compared with Alcanivorax borkumensis—the paradigm of mesophilic hydrocarbonoclastic bacteria—O. ant...
Published in: | Nature Communications |
---|---|
Main Authors: | , , , , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2013
|
Subjects: | |
Online Access: | https://doi.org/10.1038/ncomms3156 https://www.openagrar.de/receive/timport_mods_00037970 https://www.openagrar.de/servlets/MCRFileNodeServlet/timport_derivate_00037970/dn056501.pdf http://www.nature.com/ncomms/2013/130723/ncomms3156/full/ncomms3156.html |
id |
ftopenagrar:oai:www.openagrar.de:timport_mods_00037970 |
---|---|
record_format |
openpolar |
spelling |
ftopenagrar:oai:www.openagrar.de:timport_mods_00037970 2023-05-15T14:00:29+02:00 Genome sequence and functional genomic analysis of the oil-degrading bacteriumOleispira antarctica Kube, Michael Chernikova, Tatyana N. Al-Ramahi, Yamal Beloqui, Ana Lopez-Cortez, Nieves Guazzaroni, Maria-Eugenia Heipieper, Hermann J. Klages, Sven Kotsyurbenko, Oleg R. Langer, Ines Nechitaylo, Taras Y. Lünsdorf, Heinrich Fernandez, Marisol Juarez, Silvia Ciordia, Sergio Singer, Alexander U. Kagan, Olga Egorova, Olga Petit, Pierre Alain Stogios, Peter 2013 https://doi.org/10.1038/ncomms3156 https://www.openagrar.de/receive/timport_mods_00037970 https://www.openagrar.de/servlets/MCRFileNodeServlet/timport_derivate_00037970/dn056501.pdf http://www.nature.com/ncomms/2013/130723/ncomms3156/full/ncomms3156.html eng eng Nature Communications -- Nat Commun -- 2041-1723 -- 2553671-0 -- http://www.nature.com/ncomms/ -- http://www.bibliothek.uni-regensburg.de/ezeit/?2553671 -- http://d-nb.info/1002399459 https://doi.org/DOI:10.1038/ncomms3156 https://www.openagrar.de/receive/timport_mods_00037970 https://www.openagrar.de/servlets/MCRFileNodeServlet/timport_derivate_00037970/dn056501.pdf http://www.nature.com/ncomms/2013/130723/ncomms3156/full/ncomms3156.html public info:eu-repo/semantics/openAccess Text article Text 2013 ftopenagrar https://doi.org/10.1038/ncomms3156 2023-03-06T00:03:53Z Ubiquitous bacteria from the genus Oleispira drive oil degradation in the largest environment on Earth, the cold and deep sea. Here we report the genome sequence of Oleispira antarctica and show that compared with Alcanivorax borkumensis—the paradigm of mesophilic hydrocarbonoclastic bacteria—O. antarctica has a larger genome that has witnessed massive gene-transfer events. We identify an array of alkane monooxygenases, osmoprotectants, siderophores and micronutrient-scavenging pathways. We also show that at low temperatures, the main protein-folding machine Cpn60 functions as a single heptameric barrel that uses larger proteins as substrates compared with the classical double-barrel structure observed at higher temperatures. With 11 protein crystal structures, we further report the largest set of structures from one psychrotolerant organism. The most common structural feature is an increased content of surface-exposed negatively charged residues compared to their mesophilic counterparts. Our findings are relevant in the context of microbial cold-adaptation mechanisms and the development of strategies for oil-spill mitigation in cold environments. Article in Journal/Newspaper Antarc* Antarctica OpenAgrar (OA) Nature Communications 4 1 |
institution |
Open Polar |
collection |
OpenAgrar (OA) |
op_collection_id |
ftopenagrar |
language |
English |
topic |
Text |
spellingShingle |
Text Kube, Michael Chernikova, Tatyana N. Al-Ramahi, Yamal Beloqui, Ana Lopez-Cortez, Nieves Guazzaroni, Maria-Eugenia Heipieper, Hermann J. Klages, Sven Kotsyurbenko, Oleg R. Langer, Ines Nechitaylo, Taras Y. Lünsdorf, Heinrich Fernandez, Marisol Juarez, Silvia Ciordia, Sergio Singer, Alexander U. Kagan, Olga Egorova, Olga Petit, Pierre Alain Stogios, Peter Genome sequence and functional genomic analysis of the oil-degrading bacteriumOleispira antarctica |
topic_facet |
Text |
description |
Ubiquitous bacteria from the genus Oleispira drive oil degradation in the largest environment on Earth, the cold and deep sea. Here we report the genome sequence of Oleispira antarctica and show that compared with Alcanivorax borkumensis—the paradigm of mesophilic hydrocarbonoclastic bacteria—O. antarctica has a larger genome that has witnessed massive gene-transfer events. We identify an array of alkane monooxygenases, osmoprotectants, siderophores and micronutrient-scavenging pathways. We also show that at low temperatures, the main protein-folding machine Cpn60 functions as a single heptameric barrel that uses larger proteins as substrates compared with the classical double-barrel structure observed at higher temperatures. With 11 protein crystal structures, we further report the largest set of structures from one psychrotolerant organism. The most common structural feature is an increased content of surface-exposed negatively charged residues compared to their mesophilic counterparts. Our findings are relevant in the context of microbial cold-adaptation mechanisms and the development of strategies for oil-spill mitigation in cold environments. |
format |
Article in Journal/Newspaper |
author |
Kube, Michael Chernikova, Tatyana N. Al-Ramahi, Yamal Beloqui, Ana Lopez-Cortez, Nieves Guazzaroni, Maria-Eugenia Heipieper, Hermann J. Klages, Sven Kotsyurbenko, Oleg R. Langer, Ines Nechitaylo, Taras Y. Lünsdorf, Heinrich Fernandez, Marisol Juarez, Silvia Ciordia, Sergio Singer, Alexander U. Kagan, Olga Egorova, Olga Petit, Pierre Alain Stogios, Peter |
author_facet |
Kube, Michael Chernikova, Tatyana N. Al-Ramahi, Yamal Beloqui, Ana Lopez-Cortez, Nieves Guazzaroni, Maria-Eugenia Heipieper, Hermann J. Klages, Sven Kotsyurbenko, Oleg R. Langer, Ines Nechitaylo, Taras Y. Lünsdorf, Heinrich Fernandez, Marisol Juarez, Silvia Ciordia, Sergio Singer, Alexander U. Kagan, Olga Egorova, Olga Petit, Pierre Alain Stogios, Peter |
author_sort |
Kube, Michael |
title |
Genome sequence and functional genomic analysis of the oil-degrading bacteriumOleispira antarctica |
title_short |
Genome sequence and functional genomic analysis of the oil-degrading bacteriumOleispira antarctica |
title_full |
Genome sequence and functional genomic analysis of the oil-degrading bacteriumOleispira antarctica |
title_fullStr |
Genome sequence and functional genomic analysis of the oil-degrading bacteriumOleispira antarctica |
title_full_unstemmed |
Genome sequence and functional genomic analysis of the oil-degrading bacteriumOleispira antarctica |
title_sort |
genome sequence and functional genomic analysis of the oil-degrading bacteriumoleispira antarctica |
publishDate |
2013 |
url |
https://doi.org/10.1038/ncomms3156 https://www.openagrar.de/receive/timport_mods_00037970 https://www.openagrar.de/servlets/MCRFileNodeServlet/timport_derivate_00037970/dn056501.pdf http://www.nature.com/ncomms/2013/130723/ncomms3156/full/ncomms3156.html |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
Nature Communications -- Nat Commun -- 2041-1723 -- 2553671-0 -- http://www.nature.com/ncomms/ -- http://www.bibliothek.uni-regensburg.de/ezeit/?2553671 -- http://d-nb.info/1002399459 https://doi.org/DOI:10.1038/ncomms3156 https://www.openagrar.de/receive/timport_mods_00037970 https://www.openagrar.de/servlets/MCRFileNodeServlet/timport_derivate_00037970/dn056501.pdf http://www.nature.com/ncomms/2013/130723/ncomms3156/full/ncomms3156.html |
op_rights |
public info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1038/ncomms3156 |
container_title |
Nature Communications |
container_volume |
4 |
container_issue |
1 |
_version_ |
1766269648500686848 |