Increasing genomic information in bivalves through new EST T collections in four species: development of new genetic markers for environmental studies and genome evolution
The generation of EST information is an essential step in the genomic characterisation of species. In the context of the European Network Marine Genomics, a common goal was to significantly increase the amount of ESTs in commercial marine mollusk species and more specifically in the less studied but...
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ftopenagrar:oai:www.openagrar.de:timport_mods_00037542 2023-05-15T15:58:41+02:00 Increasing genomic information in bivalves through new EST T collections in four species: development of new genetic markers for environmental studies and genome evolution Tanguy, Arnaud Bierne, Nicolas Saavedra, Carlos Pina, Benjamin Bachere, Evelyne Kube, Michael Bazin, Eric Bonhomme, Francois Boudry, Pierre Boulo, Viviane Boutet, Isabelle Cancela, Leonor Dossat, Carole Favrel, Pascal Huvet, Arnaud Jarque, Sergio Jollivet, Didier Klages, Sven Lapegue, Sylvie Leite, Ricardo 2008 9 https://doi.org/10.1016/j.gene.2007.10.021 https://www.openagrar.de/receive/timport_mods_00037542 https://www.openagrar.de/servlets/MCRFileNodeServlet/timport_derivate_00037542/dn056628.pdf eng eng Gene -- Gene (Amst) -- 0378-1119 -- 1879-0038 -- 391792-7 -- 1491012-3 -- http://www.sciencedirect.com/science/journal/03781119 -- http://www.bibliothek.uni-regensburg.de/ezeit/?1491012 https://doi.org/DOI:10.1016/j.gene.2007.10.021 https://www.openagrar.de/receive/timport_mods_00037542 https://www.openagrar.de/servlets/MCRFileNodeServlet/timport_derivate_00037542/dn056628.pdf only signed in user info:eu-repo/semantics/openAccess Text molluscs ESTs environment evolution article Text 2008 ftopenagrar https://doi.org/10.1016/j.gene.2007.10.021 2023-03-06T00:03:59Z The generation of EST information is an essential step in the genomic characterisation of species. In the context of the European Network Marine Genomics, a common goal was to significantly increase the amount of ESTs in commercial marine mollusk species and more specifically in the less studied but ecologically and commercially important groups, such as mussel and clam genera. Normalized cDNA libraries were constructed for four different relevant bivalves species (Crassostrea gigas, Mytilus edulis, Ruditapes decussatus and Bathymodiolus azoricus), using numerous tissues and physiological conditions. In this paper, we present the analysis of the 13,013 expressed sequence tags (ESTs) generated. Each EST library was independently assembled and 1300–3000 unique sequences were identified in each species. For the different species, functional categories could be assigned to only about 16 to 27% of ESTs using the GO annotation tool. All sequences have been incorporated into a publicly available database and form the basis for subsequent microarray design, SNP detection and polymorphism analysis, and the placement of novel markers on genetic linkage maps. Article in Journal/Newspaper Crassostrea gigas OpenAgrar (OA) Gene 408 1-2 27 36 |
institution |
Open Polar |
collection |
OpenAgrar (OA) |
op_collection_id |
ftopenagrar |
language |
English |
topic |
Text molluscs ESTs environment evolution |
spellingShingle |
Text molluscs ESTs environment evolution Tanguy, Arnaud Bierne, Nicolas Saavedra, Carlos Pina, Benjamin Bachere, Evelyne Kube, Michael Bazin, Eric Bonhomme, Francois Boudry, Pierre Boulo, Viviane Boutet, Isabelle Cancela, Leonor Dossat, Carole Favrel, Pascal Huvet, Arnaud Jarque, Sergio Jollivet, Didier Klages, Sven Lapegue, Sylvie Leite, Ricardo Increasing genomic information in bivalves through new EST T collections in four species: development of new genetic markers for environmental studies and genome evolution |
topic_facet |
Text molluscs ESTs environment evolution |
description |
The generation of EST information is an essential step in the genomic characterisation of species. In the context of the European Network Marine Genomics, a common goal was to significantly increase the amount of ESTs in commercial marine mollusk species and more specifically in the less studied but ecologically and commercially important groups, such as mussel and clam genera. Normalized cDNA libraries were constructed for four different relevant bivalves species (Crassostrea gigas, Mytilus edulis, Ruditapes decussatus and Bathymodiolus azoricus), using numerous tissues and physiological conditions. In this paper, we present the analysis of the 13,013 expressed sequence tags (ESTs) generated. Each EST library was independently assembled and 1300–3000 unique sequences were identified in each species. For the different species, functional categories could be assigned to only about 16 to 27% of ESTs using the GO annotation tool. All sequences have been incorporated into a publicly available database and form the basis for subsequent microarray design, SNP detection and polymorphism analysis, and the placement of novel markers on genetic linkage maps. |
format |
Article in Journal/Newspaper |
author |
Tanguy, Arnaud Bierne, Nicolas Saavedra, Carlos Pina, Benjamin Bachere, Evelyne Kube, Michael Bazin, Eric Bonhomme, Francois Boudry, Pierre Boulo, Viviane Boutet, Isabelle Cancela, Leonor Dossat, Carole Favrel, Pascal Huvet, Arnaud Jarque, Sergio Jollivet, Didier Klages, Sven Lapegue, Sylvie Leite, Ricardo |
author_facet |
Tanguy, Arnaud Bierne, Nicolas Saavedra, Carlos Pina, Benjamin Bachere, Evelyne Kube, Michael Bazin, Eric Bonhomme, Francois Boudry, Pierre Boulo, Viviane Boutet, Isabelle Cancela, Leonor Dossat, Carole Favrel, Pascal Huvet, Arnaud Jarque, Sergio Jollivet, Didier Klages, Sven Lapegue, Sylvie Leite, Ricardo |
author_sort |
Tanguy, Arnaud |
title |
Increasing genomic information in bivalves through new EST T collections in four species: development of new genetic markers for environmental studies and genome evolution |
title_short |
Increasing genomic information in bivalves through new EST T collections in four species: development of new genetic markers for environmental studies and genome evolution |
title_full |
Increasing genomic information in bivalves through new EST T collections in four species: development of new genetic markers for environmental studies and genome evolution |
title_fullStr |
Increasing genomic information in bivalves through new EST T collections in four species: development of new genetic markers for environmental studies and genome evolution |
title_full_unstemmed |
Increasing genomic information in bivalves through new EST T collections in four species: development of new genetic markers for environmental studies and genome evolution |
title_sort |
increasing genomic information in bivalves through new est t collections in four species: development of new genetic markers for environmental studies and genome evolution |
publishDate |
2008 |
url |
https://doi.org/10.1016/j.gene.2007.10.021 https://www.openagrar.de/receive/timport_mods_00037542 https://www.openagrar.de/servlets/MCRFileNodeServlet/timport_derivate_00037542/dn056628.pdf |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
Gene -- Gene (Amst) -- 0378-1119 -- 1879-0038 -- 391792-7 -- 1491012-3 -- http://www.sciencedirect.com/science/journal/03781119 -- http://www.bibliothek.uni-regensburg.de/ezeit/?1491012 https://doi.org/DOI:10.1016/j.gene.2007.10.021 https://www.openagrar.de/receive/timport_mods_00037542 https://www.openagrar.de/servlets/MCRFileNodeServlet/timport_derivate_00037542/dn056628.pdf |
op_rights |
only signed in user info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.gene.2007.10.021 |
container_title |
Gene |
container_volume |
408 |
container_issue |
1-2 |
container_start_page |
27 |
op_container_end_page |
36 |
_version_ |
1766394451051151360 |