The hare syphilis agent is related to, but distinct from, the treponeme causing rabbit syphilis
The treponemes infecting lagomorphs include Treponema paraluisleporidarum ecovar Cuniculus (TPeC) and ecovar Lepus (TPeL), infecting rabbits and hares, respectively. In this study, we described the first complete genome sequence of TPeL, isolate V3603-13, from an infected mountain hare (Lepus timidu...
Published in: | PLOS ONE |
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Main Authors: | , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2024
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Subjects: |
Genomics
> Hares
> Rabbits
> Syphilis
> Genetic loci
> Nucleotides
> Nucleotide
> Sequencing
> Polymerase chain reaction
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Online Access: | https://doi.org/10.1371/journal.pone.0307196 https://www.openagrar.de/receive/openagrar_mods_00098774 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00060922/SD2024262.pdf https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0307196 |
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ftopenagrar:oai:www.openagrar.de:openagrar_mods_00098774 2024-09-15T18:17:47+00:00 The hare syphilis agent is related to, but distinct from, the treponeme causing rabbit syphilis Pospíšilová, Petra Čejková, Darina Buršíková, Pavla Fedrová, Pavla Mikalová, Lenka Najt, David Tom, Nikola Hisgen, Linda Lueert, Simone Lumeij, Johannes T. Ågren, Erik O. Knauf, Sascha Šmajs, David 2024-08-12 https://doi.org/10.1371/journal.pone.0307196 https://www.openagrar.de/receive/openagrar_mods_00098774 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00060922/SD2024262.pdf https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0307196 eng eng Public Library of Science (PLoS) PLOS ONE -- PLoS One -- PLoS one -- PLoS ONE -- 1932-6203 -- 2267670-3 -- http://journals.plos.org/plosone/ -- https://www.ncbi.nlm.nih.gov/pmc/journals/440/ -- http://www.bibliothek.uni-regensburg.de/ezeit/?2267670 https://doi.org/10.1371/journal.pone.0307196 https://www.openagrar.de/receive/openagrar_mods_00098774 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00060922/SD2024262.pdf https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0307196 https://creativecommons.org/licenses/by/4.0/ public info:eu-repo/semantics/openAccess article Text ddc:570 Genomics -- Hares -- Rabbits -- Syphilis -- Genetic loci -- Nucleotides -- Nucleotide -- Sequencing -- Polymerase chain reaction article Text doc-type:article 2024 ftopenagrar https://doi.org/10.1371/journal.pone.0307196 2024-08-19T23:38:29Z The treponemes infecting lagomorphs include Treponema paraluisleporidarum ecovar Cuniculus (TPeC) and ecovar Lepus (TPeL), infecting rabbits and hares, respectively. In this study, we described the first complete genome sequence of TPeL, isolate V3603-13, from an infected mountain hare (Lepus timidus) in Sweden. In addition, we determined 99.0% of the genome sequence of isolate V246-08 (also from an infected mountain hare, Sweden) and 31.7% of the genome sequence of isolate Z27 A77/78 (from a European hare, Lepus europeaus, The Netherlands). The TPeL V3603-13 genome had considerable gene synteny with the TPeC Cuniculi A genome and with the human pathogen T. pallidum, which causes syphilis (ssp. pallidum, TPA), yaws (ssp. pertenue, TPE) and endemic syphilis (ssp. endemicum, TEN). Compared to the TPeC Cuniculi A genome, TPeL V3603-13 contained four insertions and 11 deletions longer than three nucleotides (ranging between 6 and2,932 nts). In addition, there were 25 additional indels, from one to three nucleotides long, altogether spanning 36 nts. The number of single nucleotide variants (SNVs) between TPeC Cuniculi A and TPeL V3603-13 were represented by 309 nucleotide differences. Major proteome coding differences between TPeL and TPeC were found in the tpr gene family, and (predicted) genes coding for outer membrane proteins, suggesting that these components are essential for host adaptation in lagomorph syphilis. The phylogeny revealed that the TPeL sample from the European brown hare was more distantly related to TPeC Cuniculi A than V3603-13 and V246-08. Article in Journal/Newspaper Lepus timidus mountain hare OpenAgrar (OA) PLOS ONE 19 8 e0307196 |
institution |
Open Polar |
collection |
OpenAgrar (OA) |
op_collection_id |
ftopenagrar |
language |
English |
topic |
article Text ddc:570 Genomics -- Hares -- Rabbits -- Syphilis -- Genetic loci -- Nucleotides -- Nucleotide -- Sequencing -- Polymerase chain reaction |
spellingShingle |
article Text ddc:570 Genomics -- Hares -- Rabbits -- Syphilis -- Genetic loci -- Nucleotides -- Nucleotide -- Sequencing -- Polymerase chain reaction Pospíšilová, Petra Čejková, Darina Buršíková, Pavla Fedrová, Pavla Mikalová, Lenka Najt, David Tom, Nikola Hisgen, Linda Lueert, Simone Lumeij, Johannes T. Ågren, Erik O. Knauf, Sascha Šmajs, David The hare syphilis agent is related to, but distinct from, the treponeme causing rabbit syphilis |
topic_facet |
article Text ddc:570 Genomics -- Hares -- Rabbits -- Syphilis -- Genetic loci -- Nucleotides -- Nucleotide -- Sequencing -- Polymerase chain reaction |
description |
The treponemes infecting lagomorphs include Treponema paraluisleporidarum ecovar Cuniculus (TPeC) and ecovar Lepus (TPeL), infecting rabbits and hares, respectively. In this study, we described the first complete genome sequence of TPeL, isolate V3603-13, from an infected mountain hare (Lepus timidus) in Sweden. In addition, we determined 99.0% of the genome sequence of isolate V246-08 (also from an infected mountain hare, Sweden) and 31.7% of the genome sequence of isolate Z27 A77/78 (from a European hare, Lepus europeaus, The Netherlands). The TPeL V3603-13 genome had considerable gene synteny with the TPeC Cuniculi A genome and with the human pathogen T. pallidum, which causes syphilis (ssp. pallidum, TPA), yaws (ssp. pertenue, TPE) and endemic syphilis (ssp. endemicum, TEN). Compared to the TPeC Cuniculi A genome, TPeL V3603-13 contained four insertions and 11 deletions longer than three nucleotides (ranging between 6 and2,932 nts). In addition, there were 25 additional indels, from one to three nucleotides long, altogether spanning 36 nts. The number of single nucleotide variants (SNVs) between TPeC Cuniculi A and TPeL V3603-13 were represented by 309 nucleotide differences. Major proteome coding differences between TPeL and TPeC were found in the tpr gene family, and (predicted) genes coding for outer membrane proteins, suggesting that these components are essential for host adaptation in lagomorph syphilis. The phylogeny revealed that the TPeL sample from the European brown hare was more distantly related to TPeC Cuniculi A than V3603-13 and V246-08. |
format |
Article in Journal/Newspaper |
author |
Pospíšilová, Petra Čejková, Darina Buršíková, Pavla Fedrová, Pavla Mikalová, Lenka Najt, David Tom, Nikola Hisgen, Linda Lueert, Simone Lumeij, Johannes T. Ågren, Erik O. Knauf, Sascha Šmajs, David |
author_facet |
Pospíšilová, Petra Čejková, Darina Buršíková, Pavla Fedrová, Pavla Mikalová, Lenka Najt, David Tom, Nikola Hisgen, Linda Lueert, Simone Lumeij, Johannes T. Ågren, Erik O. Knauf, Sascha Šmajs, David |
author_sort |
Pospíšilová, Petra |
title |
The hare syphilis agent is related to, but distinct from, the treponeme causing rabbit syphilis |
title_short |
The hare syphilis agent is related to, but distinct from, the treponeme causing rabbit syphilis |
title_full |
The hare syphilis agent is related to, but distinct from, the treponeme causing rabbit syphilis |
title_fullStr |
The hare syphilis agent is related to, but distinct from, the treponeme causing rabbit syphilis |
title_full_unstemmed |
The hare syphilis agent is related to, but distinct from, the treponeme causing rabbit syphilis |
title_sort |
hare syphilis agent is related to, but distinct from, the treponeme causing rabbit syphilis |
publisher |
Public Library of Science (PLoS) |
publishDate |
2024 |
url |
https://doi.org/10.1371/journal.pone.0307196 https://www.openagrar.de/receive/openagrar_mods_00098774 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00060922/SD2024262.pdf https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0307196 |
genre |
Lepus timidus mountain hare |
genre_facet |
Lepus timidus mountain hare |
op_relation |
PLOS ONE -- PLoS One -- PLoS one -- PLoS ONE -- 1932-6203 -- 2267670-3 -- http://journals.plos.org/plosone/ -- https://www.ncbi.nlm.nih.gov/pmc/journals/440/ -- http://www.bibliothek.uni-regensburg.de/ezeit/?2267670 https://doi.org/10.1371/journal.pone.0307196 https://www.openagrar.de/receive/openagrar_mods_00098774 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00060922/SD2024262.pdf https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0307196 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ public info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0307196 |
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PLOS ONE |
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19 |
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