Deforestation for agriculture increases microbial carbon use efficiency in subarctic soils
This study investigated how and through which pathways deforestation and conversion to agricultural land (i.e. grassland, cropland) alters the microbial carbon use efficiency (CUE) in subarctic soils to allow the development of mitigation strategies to alleviate C losses. We assessed CUE using 18O-l...
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ftopenagrar:oai:www.openagrar.de:openagrar_mods_00089543 2023-10-01T03:59:43+02:00 Deforestation for agriculture increases microbial carbon use efficiency in subarctic soils Schroeder, Julia Peplau, Tino Pennekamp, Frank Gregorich, Edward Tebbe, Christoph C. Poeplau, Christopher 2022 https://doi.org/10.5281/zenodo.6720274 https://doi.org/10.5281/zenodo.6720275 https://www.openagrar.de/receive/openagrar_mods_00089543 eng eng Zenodo https://doi.org/10.5281/zenodo.6720274 https://doi.org/10.5281/zenodo.6720275 https://www.openagrar.de/receive/openagrar_mods_00089543 public https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/restrictedAccess Other ddc:333.7 Climate change -- land-use change -- structural equation modelling -- 18O-labelling method -- fungi -- archaea -- subarctic -- soil research_data 2022 ftopenagrar https://doi.org/10.5281/zenodo.672027410.5281/zenodo.6720275 2023-09-03T23:07:11Z This study investigated how and through which pathways deforestation and conversion to agricultural land (i.e. grassland, cropland) alters the microbial carbon use efficiency (CUE) in subarctic soils to allow the development of mitigation strategies to alleviate C losses. We assessed CUE using 18O-labelled water in a paired-plot approach on soils collected from 19 farms across the subarctic region of Yukon, Canada, comprising 14 pairs of forest-to-grassland conversion and 15 pairs of forest-to-cropland conversion. Microbial CUE significantly increased following conversion to grassland and cropland. Land-use conversion resulted in a lower estimated abundance of fungi, while the archaeal abundance increased, as assessed by qPCR. Interestingly, structural equation modelling revealed that increases in CUE were mediated by a rise in soil pH and a decrease in soil C:N ratio rather than by shifts in microbial community composition, i.e. the ratio of fungi, bacteria and archaea. Our findings indicate a direct control of abiotic factors on microbial CUE via improved nutrient availability and facilitated conditions for microbial growth. The R code was developed under R v3.6.3 and adapted to work under version R v.4.1.2. The repository includes the following files: general_soil_parameters_per_site.csv - general soil data assessed on pooled reference forest plot (n=19) general_soil_parameters_per_plot.csv - general soil data assessed on pooled replicated field samples (n=48) sample_data.csv - data measured for each laboratory sample (n=147) Land-use change effects on 18O-CUE.Rproj - Rproject (load project to work on provided scripts and data) load_data_script.R - loads required data Multivariate_normality_script.R - tests for multivariate normaility in dataset PCA_script.R - calculates PC1 and 2 of clay mineralogy data to reduce dimensions map_Yukon_script.R - create Figure 1 plot_density_script.R - create Figure 2 linear_mixed-effects_models_script.R - calculates response ratios plot_boxplots_script.R - plot boxplots per ... Dataset Subarctic Yukon OpenAgrar (OA) Yukon Canada |
institution |
Open Polar |
collection |
OpenAgrar (OA) |
op_collection_id |
ftopenagrar |
language |
English |
topic |
Other ddc:333.7 Climate change -- land-use change -- structural equation modelling -- 18O-labelling method -- fungi -- archaea -- subarctic -- soil |
spellingShingle |
Other ddc:333.7 Climate change -- land-use change -- structural equation modelling -- 18O-labelling method -- fungi -- archaea -- subarctic -- soil Schroeder, Julia Peplau, Tino Pennekamp, Frank Gregorich, Edward Tebbe, Christoph C. Poeplau, Christopher Deforestation for agriculture increases microbial carbon use efficiency in subarctic soils |
topic_facet |
Other ddc:333.7 Climate change -- land-use change -- structural equation modelling -- 18O-labelling method -- fungi -- archaea -- subarctic -- soil |
description |
This study investigated how and through which pathways deforestation and conversion to agricultural land (i.e. grassland, cropland) alters the microbial carbon use efficiency (CUE) in subarctic soils to allow the development of mitigation strategies to alleviate C losses. We assessed CUE using 18O-labelled water in a paired-plot approach on soils collected from 19 farms across the subarctic region of Yukon, Canada, comprising 14 pairs of forest-to-grassland conversion and 15 pairs of forest-to-cropland conversion. Microbial CUE significantly increased following conversion to grassland and cropland. Land-use conversion resulted in a lower estimated abundance of fungi, while the archaeal abundance increased, as assessed by qPCR. Interestingly, structural equation modelling revealed that increases in CUE were mediated by a rise in soil pH and a decrease in soil C:N ratio rather than by shifts in microbial community composition, i.e. the ratio of fungi, bacteria and archaea. Our findings indicate a direct control of abiotic factors on microbial CUE via improved nutrient availability and facilitated conditions for microbial growth. The R code was developed under R v3.6.3 and adapted to work under version R v.4.1.2. The repository includes the following files: general_soil_parameters_per_site.csv - general soil data assessed on pooled reference forest plot (n=19) general_soil_parameters_per_plot.csv - general soil data assessed on pooled replicated field samples (n=48) sample_data.csv - data measured for each laboratory sample (n=147) Land-use change effects on 18O-CUE.Rproj - Rproject (load project to work on provided scripts and data) load_data_script.R - loads required data Multivariate_normality_script.R - tests for multivariate normaility in dataset PCA_script.R - calculates PC1 and 2 of clay mineralogy data to reduce dimensions map_Yukon_script.R - create Figure 1 plot_density_script.R - create Figure 2 linear_mixed-effects_models_script.R - calculates response ratios plot_boxplots_script.R - plot boxplots per ... |
format |
Dataset |
author |
Schroeder, Julia Peplau, Tino Pennekamp, Frank Gregorich, Edward Tebbe, Christoph C. Poeplau, Christopher |
author_facet |
Schroeder, Julia Peplau, Tino Pennekamp, Frank Gregorich, Edward Tebbe, Christoph C. Poeplau, Christopher |
author_sort |
Schroeder, Julia |
title |
Deforestation for agriculture increases microbial carbon use efficiency in subarctic soils |
title_short |
Deforestation for agriculture increases microbial carbon use efficiency in subarctic soils |
title_full |
Deforestation for agriculture increases microbial carbon use efficiency in subarctic soils |
title_fullStr |
Deforestation for agriculture increases microbial carbon use efficiency in subarctic soils |
title_full_unstemmed |
Deforestation for agriculture increases microbial carbon use efficiency in subarctic soils |
title_sort |
deforestation for agriculture increases microbial carbon use efficiency in subarctic soils |
publisher |
Zenodo |
publishDate |
2022 |
url |
https://doi.org/10.5281/zenodo.6720274 https://doi.org/10.5281/zenodo.6720275 https://www.openagrar.de/receive/openagrar_mods_00089543 |
geographic |
Yukon Canada |
geographic_facet |
Yukon Canada |
genre |
Subarctic Yukon |
genre_facet |
Subarctic Yukon |
op_relation |
https://doi.org/10.5281/zenodo.6720274 https://doi.org/10.5281/zenodo.6720275 https://www.openagrar.de/receive/openagrar_mods_00089543 |
op_rights |
public https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.5281/zenodo.672027410.5281/zenodo.6720275 |
_version_ |
1778534076618113024 |