Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe
Bat-associated hantaviruses have been detected in Asia, Africa and Europe. Recently, a novel hantavirus (Brno loanvirus, BRNV) was identified in common noctule bats (Nyctalus noctula) in the Czech Republic, but nothing is known about its geographical range and prevalence. The objective of this study...
Published in: | Virus Genes |
---|---|
Main Authors: | , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Springer Science and Business Media LLC
2022
|
Subjects: | |
Online Access: | https://doi.org/10.1007/s11262-022-01952-2 https://www.openagrar.de/receive/openagrar_mods_00084564 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00052214/SD2022496_SD2023103.pdf https://link.springer.com/content/pdf/10.1007/s11262-022-01952-2.pdf https://link.springer.com/article/10.1007/s11262-022-01952-2 |
id |
ftopenagrar:oai:www.openagrar.de:openagrar_mods_00084564 |
---|---|
record_format |
openpolar |
spelling |
ftopenagrar:oai:www.openagrar.de:openagrar_mods_00084564 2024-09-15T18:27:23+00:00 Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe Dafalla, Maysaa Abdulla Mohammed Orłowska, Anna Keleş, Sinan Julian Straková, Petra Schlottau, Kore Jeske, Kathrin Hoffmann, Bernd Wibbelt, Gudrun Smreczak, Marcin Müller, Thomas Freuling, Conrad Martin Wang, Xuejing Rola, Jerzy Drewes, Stephan Fereidouni, Sasan R. Heckel, Gerald Ulrich, Rainer G. 2022-12-21 https://doi.org/10.1007/s11262-022-01952-2 https://www.openagrar.de/receive/openagrar_mods_00084564 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00052214/SD2022496_SD2023103.pdf https://link.springer.com/content/pdf/10.1007/s11262-022-01952-2.pdf https://link.springer.com/article/10.1007/s11262-022-01952-2 eng eng Springer Science and Business Media LLC Virus Genes -- 1572-994X -- 0920-8569 -- 2011138-1 -- 639496-6 -- http://link.springer.com/journal/11262 -- http://www.bibliothek.uni-regensburg.de/ezeit/?2011138 -- http://d-nb.info/020340982 https://doi.org/10.1007/s11262-022-01952-2 https://www.openagrar.de/receive/openagrar_mods_00084564 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00052214/SD2022496_SD2023103.pdf https://link.springer.com/content/pdf/10.1007/s11262-022-01952-2.pdf https://link.springer.com/article/10.1007/s11262-022-01952-2 https://creativecommons.org/licenses/by/4.0/ public info:eu-repo/semantics/openAccess article Text ddc:570 Bats -- Europe -- Hantavirus -- Host -- Host specificity -- RT-qPCR article Text doc-type:article 2022 ftopenagrar https://doi.org/10.1007/s11262-022-01952-2 2024-07-08T23:56:24Z Bat-associated hantaviruses have been detected in Asia, Africa and Europe. Recently, a novel hantavirus (Brno loanvirus, BRNV) was identified in common noctule bats (Nyctalus noctula) in the Czech Republic, but nothing is known about its geographical range and prevalence. The objective of this study was to evaluate the distribution and host specificity of BRNV by testing bats from neighbouring countries Germany, Austria and Poland. One thousand forty-seven bats representing 21 species from Germany, 464 bats representing 18 species from Austria and 77 bats representing 12 species from Poland were screened by L segment broad-spectrum nested reverse transcription-polymerase chain reaction (RT-PCR) or by BRNV-specific real-time RT-PCR. Three common noctules from Germany, one common noctule from Austria and three common noctules from Poland were positive in the hantavirus RNA screening. Conventional RT-PCR and primer walking resulted in the amplification of partial L segment and (almost) complete S and M segment coding sequences for samples from Germany and partial L segment sequences for samples from Poland. Phylogenetic analysis of these nucleotide sequences showed highest similarity to BRNV from Czech Republic. The exclusive detection of BRNV in common noctules from different countries suggests high host specificity. The RNA detection rate in common noctules ranged between 1 of 207 (0.5%; Austria), 3 of 245 (1.2%; Germany) and 3 of 20 (15%; Poland). In conclusion, this study demonstrates a broader distribution of BRNV in common noctules in Central Europe, but at low to moderate prevalence. Additional studies are needed to prove the zoonotic potential of this hantavirus and evaluate its transmission within bat populations. Article in Journal/Newspaper Nyctalus noctula OpenAgrar (OA) Virus Genes |
institution |
Open Polar |
collection |
OpenAgrar (OA) |
op_collection_id |
ftopenagrar |
language |
English |
topic |
article Text ddc:570 Bats -- Europe -- Hantavirus -- Host -- Host specificity -- RT-qPCR |
spellingShingle |
article Text ddc:570 Bats -- Europe -- Hantavirus -- Host -- Host specificity -- RT-qPCR Dafalla, Maysaa Abdulla Mohammed Orłowska, Anna Keleş, Sinan Julian Straková, Petra Schlottau, Kore Jeske, Kathrin Hoffmann, Bernd Wibbelt, Gudrun Smreczak, Marcin Müller, Thomas Freuling, Conrad Martin Wang, Xuejing Rola, Jerzy Drewes, Stephan Fereidouni, Sasan R. Heckel, Gerald Ulrich, Rainer G. Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe |
topic_facet |
article Text ddc:570 Bats -- Europe -- Hantavirus -- Host -- Host specificity -- RT-qPCR |
description |
Bat-associated hantaviruses have been detected in Asia, Africa and Europe. Recently, a novel hantavirus (Brno loanvirus, BRNV) was identified in common noctule bats (Nyctalus noctula) in the Czech Republic, but nothing is known about its geographical range and prevalence. The objective of this study was to evaluate the distribution and host specificity of BRNV by testing bats from neighbouring countries Germany, Austria and Poland. One thousand forty-seven bats representing 21 species from Germany, 464 bats representing 18 species from Austria and 77 bats representing 12 species from Poland were screened by L segment broad-spectrum nested reverse transcription-polymerase chain reaction (RT-PCR) or by BRNV-specific real-time RT-PCR. Three common noctules from Germany, one common noctule from Austria and three common noctules from Poland were positive in the hantavirus RNA screening. Conventional RT-PCR and primer walking resulted in the amplification of partial L segment and (almost) complete S and M segment coding sequences for samples from Germany and partial L segment sequences for samples from Poland. Phylogenetic analysis of these nucleotide sequences showed highest similarity to BRNV from Czech Republic. The exclusive detection of BRNV in common noctules from different countries suggests high host specificity. The RNA detection rate in common noctules ranged between 1 of 207 (0.5%; Austria), 3 of 245 (1.2%; Germany) and 3 of 20 (15%; Poland). In conclusion, this study demonstrates a broader distribution of BRNV in common noctules in Central Europe, but at low to moderate prevalence. Additional studies are needed to prove the zoonotic potential of this hantavirus and evaluate its transmission within bat populations. |
format |
Article in Journal/Newspaper |
author |
Dafalla, Maysaa Abdulla Mohammed Orłowska, Anna Keleş, Sinan Julian Straková, Petra Schlottau, Kore Jeske, Kathrin Hoffmann, Bernd Wibbelt, Gudrun Smreczak, Marcin Müller, Thomas Freuling, Conrad Martin Wang, Xuejing Rola, Jerzy Drewes, Stephan Fereidouni, Sasan R. Heckel, Gerald Ulrich, Rainer G. |
author_facet |
Dafalla, Maysaa Abdulla Mohammed Orłowska, Anna Keleş, Sinan Julian Straková, Petra Schlottau, Kore Jeske, Kathrin Hoffmann, Bernd Wibbelt, Gudrun Smreczak, Marcin Müller, Thomas Freuling, Conrad Martin Wang, Xuejing Rola, Jerzy Drewes, Stephan Fereidouni, Sasan R. Heckel, Gerald Ulrich, Rainer G. |
author_sort |
Dafalla, Maysaa Abdulla Mohammed |
title |
Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe |
title_short |
Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe |
title_full |
Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe |
title_fullStr |
Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe |
title_full_unstemmed |
Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe |
title_sort |
hantavirus brno loanvirus is highly specific to the common noctule bat (nyctalus noctula) and widespread in central europe |
publisher |
Springer Science and Business Media LLC |
publishDate |
2022 |
url |
https://doi.org/10.1007/s11262-022-01952-2 https://www.openagrar.de/receive/openagrar_mods_00084564 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00052214/SD2022496_SD2023103.pdf https://link.springer.com/content/pdf/10.1007/s11262-022-01952-2.pdf https://link.springer.com/article/10.1007/s11262-022-01952-2 |
genre |
Nyctalus noctula |
genre_facet |
Nyctalus noctula |
op_relation |
Virus Genes -- 1572-994X -- 0920-8569 -- 2011138-1 -- 639496-6 -- http://link.springer.com/journal/11262 -- http://www.bibliothek.uni-regensburg.de/ezeit/?2011138 -- http://d-nb.info/020340982 https://doi.org/10.1007/s11262-022-01952-2 https://www.openagrar.de/receive/openagrar_mods_00084564 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00052214/SD2022496_SD2023103.pdf https://link.springer.com/content/pdf/10.1007/s11262-022-01952-2.pdf https://link.springer.com/article/10.1007/s11262-022-01952-2 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ public info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1007/s11262-022-01952-2 |
container_title |
Virus Genes |
_version_ |
1810468610153381888 |