Diversity of Borrelia burgdorferi sensu lato in ticks and small mammals from different habitats

• Published in: Parasites & Vectors
• Main Authors: Król, Nina, Obiegala, Anna, Imholt, Christian, Arz, Charlotte, Schmidt, Elisabeth, Jeske, Kathrin, Ulrich, Rainer Günter, Rentería-Solís, Zaida, Jacob, Jens, Pfeffer, Martin
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2022
• Subjects: article; Text; ddc:570; ddc:630; Germany; Grassland; Forest; Sequence type; Multilocus sequence typing; Clethrionomys; Microtus; Apodemus; Sorex; Ixodes; Common vole; Microtus arvalis
• Online Access: https://doi.org/10.1186/s13071-022-05326-3; https://www.openagrar.de/receive/openagrar_mods_00080127; https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00046574/SD2022217.pdf; https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-022-05326-3; https://link.springer.com/article/10.1186/s13071-022-05326-3

Background: Ixodid ticks are important vectors for zoonotic pathogens, with Ixodes ricinus being the most important in Europe. Rodents are hosts of immature life stages of I. ricinus ticks and are considered main reservoirs for tick-borne pathogens, e.g. Borrelia burgdorferi. The aim of this study was to analyse the prevalence as well as genospecies and sequence type (ST) diversity of Borrelia burgdorferi sensu lato in ticks and small mammals from central Germany and to elaborate on the influence of environmental and/or individual host and vector factors on Borrelia prevalence. Methods: After species identification, 1167 small mammal skin samples and 1094 ticks from vegetation were screened by B. burgdorferi sensu lato real-time polymerase chain reaction, and positive samples were characterized by multilocus sequence typing. Generalized linear (mixed) models were used to estimate how seasonality, small mammal species/tick life stage and habitat affect individual infection status. Results: In total, 10 small mammal species and three tick species, Ixodes ricinus, Ixodes inopinatus (both considered members of the I. ricinus complex) and Dermacentor reticulatus, were investigated. Borrelia DNA was detected in eight host species, i.e. the striped field mouse (Apodemus agrarius), the yellow-necked field mouse (Apodemus flavicollis), the wood mouse (Apodemus sylvaticus), the water vole (Arvicola amphibius), the bank vole (Clethrionomys glareolus), the field vole (Microtus agrestis), the common vole (Microtus arvalis), and the common shrew (Sorex araneus). Two species were Borrelia negative, the greater white-toothed shrew (Crocidura russula) and the pygmy shrew (Sorex minutus). The average prevalence was 6.2%, with two genospecies detected, Borrelia afzelii and Borrelia garinii, and at least three STs that had not been previously reported in small mammals. Borrelia prevalence in small mammals did not differ between seasons. Six genospecies of Borrelia—Borrelia afzelii, Borrelia valaisiana, Borrelia garinii, Borrelia ...