The methodologies and application potential of genetically modified farm animals
The first transgenic livestock were produced in the mid-1980s by microinjection of foreign DNA into zygotic pronuclei and remained the method of choice for more than 15 years, when it was gradually replaced by functional somatic cell nuclear transfer (SCNT) protocols. Recently, molecular scissors, i...
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ftopenagrar:oai:www.openagrar.de:openagrar_mods_00056989 2023-05-15T15:32:42+02:00 The methodologies and application potential of genetically modified farm animals Niemann, Heiner Lucas-Hahn, Andrea Petersen, Björn 2019-07-31 https://doi.org/10.1016/B978-0-444-64046-8.00292-5 https://www.openagrar.de/receive/openagrar_mods_00056989 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00042439/SD2019533.pdf https://www.sciencedirect.com/science/article/pii/B9780444640468002925 eng eng Comprehensive Biotechnology -- https://www.sciencedirect.com/referencework/9780444640475/comprehensive-biotechnology -- 978-0-444-64047-5 -- 978-0-444-64046-8 https://doi.org/10.1016/B978-0-444-64046-8.00292-5 https://www.openagrar.de/receive/openagrar_mods_00056989 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00042439/SD2019533.pdf https://www.sciencedirect.com/science/article/pii/B9780444640468002925 all rights reserved only signed in user info:eu-repo/semantics/openAccess Text ddc:570 DNA microinjection into pronuclei of zygotes DNA nucleases Gene editing Gene targeting Homology directed repair (HDR) Nonhomologous endjoining (NHEJ) Precision breeding concepts Somatic cell nuclear transfer (SCNT) Transgenic farm animals for biomedicine and agriculture Transgenic pigs for human disease models Xenotransplantation entry Text 2019 ftopenagrar https://doi.org/10.1016/B978-0-444-64046-8.00292-5 2023-02-20T00:16:41Z The first transgenic livestock were produced in the mid-1980s by microinjection of foreign DNA into zygotic pronuclei and remained the method of choice for more than 15 years, when it was gradually replaced by functional somatic cell nuclear transfer (SCNT) protocols. Recently, molecular scissors, including Zinc Finger Nucleases (ZFN), Transcription Activator-like Effector Nucleases (TALEN) and the CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats) system have been successfully applied in the livestock species and were shown to allow precise modifications of the genome. Although the efficiency of transgenic animal production by microinjection technology was low, many animals with agriculturally important transgenic traits were produced by this method, including improved carcass composition, lactational performance, wool production, enhanced disease resistance and reduced environmental impact. However, none of the transgenic livestock with enhanced production traits has been accepted for commercial use, with the exception of the AquaAdvantage Atlantic salmon from the company Aquabounty. In contrast, at least three recombinant pharmaceutical proteins produced from the mammary gland of transgenic animals were approved by the supervisory agencies in Europe and North America. Moreover, numerous transgenic pig models for important human diseases have been produced and pigs with multiple genetic modifications have contributed to significant progress in xenotransplantation research. The use of DNA nucleases will pave the way to precision breeding concepts in livestock. Text Atlantic salmon OpenAgrar (OA) 466 480 |
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Text ddc:570 DNA microinjection into pronuclei of zygotes DNA nucleases Gene editing Gene targeting Homology directed repair (HDR) Nonhomologous endjoining (NHEJ) Precision breeding concepts Somatic cell nuclear transfer (SCNT) Transgenic farm animals for biomedicine and agriculture Transgenic pigs for human disease models Xenotransplantation |
spellingShingle |
Text ddc:570 DNA microinjection into pronuclei of zygotes DNA nucleases Gene editing Gene targeting Homology directed repair (HDR) Nonhomologous endjoining (NHEJ) Precision breeding concepts Somatic cell nuclear transfer (SCNT) Transgenic farm animals for biomedicine and agriculture Transgenic pigs for human disease models Xenotransplantation Niemann, Heiner Lucas-Hahn, Andrea Petersen, Björn The methodologies and application potential of genetically modified farm animals |
topic_facet |
Text ddc:570 DNA microinjection into pronuclei of zygotes DNA nucleases Gene editing Gene targeting Homology directed repair (HDR) Nonhomologous endjoining (NHEJ) Precision breeding concepts Somatic cell nuclear transfer (SCNT) Transgenic farm animals for biomedicine and agriculture Transgenic pigs for human disease models Xenotransplantation |
description |
The first transgenic livestock were produced in the mid-1980s by microinjection of foreign DNA into zygotic pronuclei and remained the method of choice for more than 15 years, when it was gradually replaced by functional somatic cell nuclear transfer (SCNT) protocols. Recently, molecular scissors, including Zinc Finger Nucleases (ZFN), Transcription Activator-like Effector Nucleases (TALEN) and the CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats) system have been successfully applied in the livestock species and were shown to allow precise modifications of the genome. Although the efficiency of transgenic animal production by microinjection technology was low, many animals with agriculturally important transgenic traits were produced by this method, including improved carcass composition, lactational performance, wool production, enhanced disease resistance and reduced environmental impact. However, none of the transgenic livestock with enhanced production traits has been accepted for commercial use, with the exception of the AquaAdvantage Atlantic salmon from the company Aquabounty. In contrast, at least three recombinant pharmaceutical proteins produced from the mammary gland of transgenic animals were approved by the supervisory agencies in Europe and North America. Moreover, numerous transgenic pig models for important human diseases have been produced and pigs with multiple genetic modifications have contributed to significant progress in xenotransplantation research. The use of DNA nucleases will pave the way to precision breeding concepts in livestock. |
format |
Text |
author |
Niemann, Heiner Lucas-Hahn, Andrea Petersen, Björn |
author_facet |
Niemann, Heiner Lucas-Hahn, Andrea Petersen, Björn |
author_sort |
Niemann, Heiner |
title |
The methodologies and application potential of genetically modified farm animals |
title_short |
The methodologies and application potential of genetically modified farm animals |
title_full |
The methodologies and application potential of genetically modified farm animals |
title_fullStr |
The methodologies and application potential of genetically modified farm animals |
title_full_unstemmed |
The methodologies and application potential of genetically modified farm animals |
title_sort |
methodologies and application potential of genetically modified farm animals |
publishDate |
2019 |
url |
https://doi.org/10.1016/B978-0-444-64046-8.00292-5 https://www.openagrar.de/receive/openagrar_mods_00056989 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00042439/SD2019533.pdf https://www.sciencedirect.com/science/article/pii/B9780444640468002925 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
Comprehensive Biotechnology -- https://www.sciencedirect.com/referencework/9780444640475/comprehensive-biotechnology -- 978-0-444-64047-5 -- 978-0-444-64046-8 https://doi.org/10.1016/B978-0-444-64046-8.00292-5 https://www.openagrar.de/receive/openagrar_mods_00056989 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00042439/SD2019533.pdf https://www.sciencedirect.com/science/article/pii/B9780444640468002925 |
op_rights |
all rights reserved only signed in user info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/B978-0-444-64046-8.00292-5 |
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