Genomic and spatial variability of a European common vole hepevirus
Rodents host different orthohepeviruses, namely orthohepevirus C genotype HEV-C1 (rat hepatitis E virus, HEV) and the additional putative genotypes HEV-C3 and HEV-C4. Here, we screened 2,961 rodents from Central Europe by reverse transcription polymerase chain reaction (RT-PCR) and identified HEV RN...
Published in: | Archives of Virology |
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ftopenagrar:oai:www.openagrar.de:openagrar_mods_00050548 2024-09-09T19:36:57+00:00 Genomic and spatial variability of a European common vole hepevirus Ryll, René Heckel, Gerald Corman, Victor M. Drexler, Jan Felix Ulrich, Rainer 2019-08-09 https://doi.org/10.1007/s00705-019-04347-1 https://www.openagrar.de/receive/openagrar_mods_00050548 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00023683/SD2019310.pdf https://link.springer.com/article/10.1007/s00705-019-04347 eng eng Springer Science and Business Media LLC Archives of Virology -- Arch Virol -- 1432-8798 -- 0304-8608 -- 1458460-8 -- 7491-3 -- http://link.springer.com/journal/705 -- http://www.bibliothek.uni-regensburg.de/ezeit/?1458460 https://doi.org/10.1007/s00705-019-04347-1 https://www.openagrar.de/receive/openagrar_mods_00050548 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00023683/SD2019310.pdf https://link.springer.com/article/10.1007/s00705-019-04347 all rights reserved only signed in user info:eu-repo/semantics/restrictedAccess article Text ddc:570 article Text doc-type:article 2019 ftopenagrar https://doi.org/10.1007/s00705-019-04347-1 2024-07-08T23:56:25Z Rodents host different orthohepeviruses, namely orthohepevirus C genotype HEV-C1 (rat hepatitis E virus, HEV) and the additional putative genotypes HEV-C3 and HEV-C4. Here, we screened 2,961 rodents from Central Europe by reverse transcription polymerase chain reaction (RT-PCR) and identified HEV RNA in 13 common voles (Microtus arvalis) and one bank vole (Myodes glareolus) with detection rates of 2% (95% confidence interval [CI]: 1-3.4) and 0.08% (95% CI: 0.002-0.46), respectively. Sequencing of a 279-nucleotide RT-PCR amplicon corresponding to a region within open reading frame (ORF) 1 showed a high degree of similarity to recently described common vole-associated HEV (cvHEV) sequences from Hungary. Five novel complete cvHEV genome sequences from Central Europe showed the typical HEV genome organization with ORF1, ORF2 and ORF3 and RNA secondary structure. Uncommon features included a noncanonical start codon in ORF3, multiple insertions and deletions within ORF1 and ORF2/ORF3, and the absence of a putative ORF4. Phylogenetic analysis showed all of the novel cvHEV sequences to be monophyletic, clustering most closely with an unassigned bird-derived sequence and other sequences of the species Orthohepevirus C. The nucleotide and amino acid sequence divergence of the common vole-derived sequences was significantly correlated with the spatial distance between the trapping sites, indicating mostly local evolutionary processes. Detection of closely related HEV sequences in common voles in multiple localities over a distance of 800 kilometers suggested that common voles are infected by cvHEV across broad geographic distances. The common vole-associated HEV strain is clearly divergent from HEV sequences recently found in narrow-headed voles (Microtus gregalis) and other cricetid rodents. Article in Journal/Newspaper Common vole Microtus arvalis OpenAgrar (OA) Archives of Virology 164 11 2671 2682 |
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article Text ddc:570 Ryll, René Heckel, Gerald Corman, Victor M. Drexler, Jan Felix Ulrich, Rainer Genomic and spatial variability of a European common vole hepevirus |
topic_facet |
article Text ddc:570 |
description |
Rodents host different orthohepeviruses, namely orthohepevirus C genotype HEV-C1 (rat hepatitis E virus, HEV) and the additional putative genotypes HEV-C3 and HEV-C4. Here, we screened 2,961 rodents from Central Europe by reverse transcription polymerase chain reaction (RT-PCR) and identified HEV RNA in 13 common voles (Microtus arvalis) and one bank vole (Myodes glareolus) with detection rates of 2% (95% confidence interval [CI]: 1-3.4) and 0.08% (95% CI: 0.002-0.46), respectively. Sequencing of a 279-nucleotide RT-PCR amplicon corresponding to a region within open reading frame (ORF) 1 showed a high degree of similarity to recently described common vole-associated HEV (cvHEV) sequences from Hungary. Five novel complete cvHEV genome sequences from Central Europe showed the typical HEV genome organization with ORF1, ORF2 and ORF3 and RNA secondary structure. Uncommon features included a noncanonical start codon in ORF3, multiple insertions and deletions within ORF1 and ORF2/ORF3, and the absence of a putative ORF4. Phylogenetic analysis showed all of the novel cvHEV sequences to be monophyletic, clustering most closely with an unassigned bird-derived sequence and other sequences of the species Orthohepevirus C. The nucleotide and amino acid sequence divergence of the common vole-derived sequences was significantly correlated with the spatial distance between the trapping sites, indicating mostly local evolutionary processes. Detection of closely related HEV sequences in common voles in multiple localities over a distance of 800 kilometers suggested that common voles are infected by cvHEV across broad geographic distances. The common vole-associated HEV strain is clearly divergent from HEV sequences recently found in narrow-headed voles (Microtus gregalis) and other cricetid rodents. |
format |
Article in Journal/Newspaper |
author |
Ryll, René Heckel, Gerald Corman, Victor M. Drexler, Jan Felix Ulrich, Rainer |
author_facet |
Ryll, René Heckel, Gerald Corman, Victor M. Drexler, Jan Felix Ulrich, Rainer |
author_sort |
Ryll, René |
title |
Genomic and spatial variability of a European common vole hepevirus |
title_short |
Genomic and spatial variability of a European common vole hepevirus |
title_full |
Genomic and spatial variability of a European common vole hepevirus |
title_fullStr |
Genomic and spatial variability of a European common vole hepevirus |
title_full_unstemmed |
Genomic and spatial variability of a European common vole hepevirus |
title_sort |
genomic and spatial variability of a european common vole hepevirus |
publisher |
Springer Science and Business Media LLC |
publishDate |
2019 |
url |
https://doi.org/10.1007/s00705-019-04347-1 https://www.openagrar.de/receive/openagrar_mods_00050548 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00023683/SD2019310.pdf https://link.springer.com/article/10.1007/s00705-019-04347 |
genre |
Common vole Microtus arvalis |
genre_facet |
Common vole Microtus arvalis |
op_relation |
Archives of Virology -- Arch Virol -- 1432-8798 -- 0304-8608 -- 1458460-8 -- 7491-3 -- http://link.springer.com/journal/705 -- http://www.bibliothek.uni-regensburg.de/ezeit/?1458460 https://doi.org/10.1007/s00705-019-04347-1 https://www.openagrar.de/receive/openagrar_mods_00050548 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00023683/SD2019310.pdf https://link.springer.com/article/10.1007/s00705-019-04347 |
op_rights |
all rights reserved only signed in user info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1007/s00705-019-04347-1 |
container_title |
Archives of Virology |
container_volume |
164 |
container_issue |
11 |
container_start_page |
2671 |
op_container_end_page |
2682 |
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1809906070440640512 |