Antigenic Characterization of Street Rabies Virus Isolates from Nigeria Using Monoclonal-Antibodies
Twenty isolates of street rabies virus were recovered in mouse neutroblastoma cells from 84 rabies suspect brain specimens from Nigeria dogs and a cat. They were characterized with the Tübingen monoclonal antibody panels directed against nucleocapsid and glycoprotein antigens. Antigenic variatons we...
Published in: | Journal of Veterinary Medicine, Series B |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
1990
|
Subjects: | |
Online Access: | https://doi.org/10.1111/j.1439-0450.1990.tb01050.x https://www.openagrar.de/receive/openagrar_mods_00049289 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00021791/SD199040.pdf |
id |
ftopenagrar:oai:www.openagrar.de:openagrar_mods_00049289 |
---|---|
record_format |
openpolar |
spelling |
ftopenagrar:oai:www.openagrar.de:openagrar_mods_00049289 2023-05-15T15:25:05+02:00 Antigenic Characterization of Street Rabies Virus Isolates from Nigeria Using Monoclonal-Antibodies Umoh, J. U. Cox, James H. Schneider, Lothar 1990 https://doi.org/10.1111/j.1439-0450.1990.tb01050.x https://www.openagrar.de/receive/openagrar_mods_00049289 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00021791/SD199040.pdf eng eng Journal of veterinary medicine / Series B, Infectious diseases, immunobiology, food hygiene, public health -- Zentralblatt für Veterinärmedizin -- J Vet Med B Infect Dis Vet Public Health -- 0931-1793 -- 0721-1856 -- 1439-0450 -- 632892-1 -- 2020603-3 https://doi.org/10.1111/j.1439-0450.1990.tb01050.x https://www.openagrar.de/receive/openagrar_mods_00049289 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00021791/SD199040.pdf only signed in user all rights reserved info:eu-repo/semantics/openAccess Text ddc:570 article Text 1990 ftopenagrar https://doi.org/10.1111/j.1439-0450.1990.tb01050.x 2023-02-20T00:17:41Z Twenty isolates of street rabies virus were recovered in mouse neutroblastoma cells from 84 rabies suspect brain specimens from Nigeria dogs and a cat. They were characterized with the Tübingen monoclonal antibody panels directed against nucleocapsid and glycoprotein antigens. Antigenic variatons were detected both with antinucleocapsid (anti‐NC) and antiglycoprotein (anti‐GP) monoclonal antibodies (mabs). One isolate reacted positively with anti‐NC mab P41 which hitherto has been known to react positively with polar rabies. Another isolate did not react with anti‐NC mab 187.5; a reaction normally seen with ERA/SAD strains of rabies virus. With anti‐GP mabs it was possible to group the isolates by their area of origin. Isolates from Plateau State were not neutralized by anti‐GP mabs ERA 543 and P44.7.2. The isolates studied had glycoprotein antigenic patterns different from the pattern for low egg passage (LEP) Flury strain vaccine virus used in Nigeria for immunization of dogs. Aus Gehirnproben von 84 Hunden und einer Katze aus Nigeria, in denen Tollwut‐Straßenvirus vermutet wurde, konnten 20 Isolate an Maus‐Neuroblastomzellen angepaßt werden. Diese wurden mit den in Tübingen verwendeten monoklonalen Antikörpern (MKA) auf Nukleokapsid‐ und Glykoproteinantigene charakterisiert. Antigenunterschiede gab es mit Antinukleokapsid‐MKA und Antiglykoprotein‐MKA. Ein Isolat reagierte positiv mit Antinukleokapsid‐MKA P41, das üblicher‐weise mit Isolaten der arktischen Tollwut positiv reagiert. Ein anderes Isolat reagierte nicht mit Antinukleokapsid‐MKA 187.5, eine Reaktion, die man üblicherweise bei ERA/SAD‐Stämmen des Tollwutvirus sieht. Mit Antiglykoprotein‐MKA war es möglich, die Isolate nach der geographischen Herkunft einzuordnen. Isolate vom Plateau State (Nigeria) wurden von den Antiglykoprotein‐MKA ERA 543 und P44.7.2 nicht neutralisiert. Die untersuchten Isolate hatten ein Glykoproteinantigenmuster, das unterschiedlich war vom Muster für den Low Egg Passage (LEP) Flury‐Stamm, der in Nigeria zur Herstellung von ... Article in Journal/Newspaper Arktis* OpenAgrar (OA) Journal of Veterinary Medicine, Series B 37 1-10 222 228 |
institution |
Open Polar |
collection |
OpenAgrar (OA) |
op_collection_id |
ftopenagrar |
language |
English |
topic |
Text ddc:570 |
spellingShingle |
Text ddc:570 Umoh, J. U. Cox, James H. Schneider, Lothar Antigenic Characterization of Street Rabies Virus Isolates from Nigeria Using Monoclonal-Antibodies |
topic_facet |
Text ddc:570 |
description |
Twenty isolates of street rabies virus were recovered in mouse neutroblastoma cells from 84 rabies suspect brain specimens from Nigeria dogs and a cat. They were characterized with the Tübingen monoclonal antibody panels directed against nucleocapsid and glycoprotein antigens. Antigenic variatons were detected both with antinucleocapsid (anti‐NC) and antiglycoprotein (anti‐GP) monoclonal antibodies (mabs). One isolate reacted positively with anti‐NC mab P41 which hitherto has been known to react positively with polar rabies. Another isolate did not react with anti‐NC mab 187.5; a reaction normally seen with ERA/SAD strains of rabies virus. With anti‐GP mabs it was possible to group the isolates by their area of origin. Isolates from Plateau State were not neutralized by anti‐GP mabs ERA 543 and P44.7.2. The isolates studied had glycoprotein antigenic patterns different from the pattern for low egg passage (LEP) Flury strain vaccine virus used in Nigeria for immunization of dogs. Aus Gehirnproben von 84 Hunden und einer Katze aus Nigeria, in denen Tollwut‐Straßenvirus vermutet wurde, konnten 20 Isolate an Maus‐Neuroblastomzellen angepaßt werden. Diese wurden mit den in Tübingen verwendeten monoklonalen Antikörpern (MKA) auf Nukleokapsid‐ und Glykoproteinantigene charakterisiert. Antigenunterschiede gab es mit Antinukleokapsid‐MKA und Antiglykoprotein‐MKA. Ein Isolat reagierte positiv mit Antinukleokapsid‐MKA P41, das üblicher‐weise mit Isolaten der arktischen Tollwut positiv reagiert. Ein anderes Isolat reagierte nicht mit Antinukleokapsid‐MKA 187.5, eine Reaktion, die man üblicherweise bei ERA/SAD‐Stämmen des Tollwutvirus sieht. Mit Antiglykoprotein‐MKA war es möglich, die Isolate nach der geographischen Herkunft einzuordnen. Isolate vom Plateau State (Nigeria) wurden von den Antiglykoprotein‐MKA ERA 543 und P44.7.2 nicht neutralisiert. Die untersuchten Isolate hatten ein Glykoproteinantigenmuster, das unterschiedlich war vom Muster für den Low Egg Passage (LEP) Flury‐Stamm, der in Nigeria zur Herstellung von ... |
format |
Article in Journal/Newspaper |
author |
Umoh, J. U. Cox, James H. Schneider, Lothar |
author_facet |
Umoh, J. U. Cox, James H. Schneider, Lothar |
author_sort |
Umoh, J. U. |
title |
Antigenic Characterization of Street Rabies Virus Isolates from Nigeria Using Monoclonal-Antibodies |
title_short |
Antigenic Characterization of Street Rabies Virus Isolates from Nigeria Using Monoclonal-Antibodies |
title_full |
Antigenic Characterization of Street Rabies Virus Isolates from Nigeria Using Monoclonal-Antibodies |
title_fullStr |
Antigenic Characterization of Street Rabies Virus Isolates from Nigeria Using Monoclonal-Antibodies |
title_full_unstemmed |
Antigenic Characterization of Street Rabies Virus Isolates from Nigeria Using Monoclonal-Antibodies |
title_sort |
antigenic characterization of street rabies virus isolates from nigeria using monoclonal-antibodies |
publishDate |
1990 |
url |
https://doi.org/10.1111/j.1439-0450.1990.tb01050.x https://www.openagrar.de/receive/openagrar_mods_00049289 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00021791/SD199040.pdf |
genre |
Arktis* |
genre_facet |
Arktis* |
op_relation |
Journal of veterinary medicine / Series B, Infectious diseases, immunobiology, food hygiene, public health -- Zentralblatt für Veterinärmedizin -- J Vet Med B Infect Dis Vet Public Health -- 0931-1793 -- 0721-1856 -- 1439-0450 -- 632892-1 -- 2020603-3 https://doi.org/10.1111/j.1439-0450.1990.tb01050.x https://www.openagrar.de/receive/openagrar_mods_00049289 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00021791/SD199040.pdf |
op_rights |
only signed in user all rights reserved info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1111/j.1439-0450.1990.tb01050.x |
container_title |
Journal of Veterinary Medicine, Series B |
container_volume |
37 |
container_issue |
1-10 |
container_start_page |
222 |
op_container_end_page |
228 |
_version_ |
1766355720726380544 |