Detection of Herpesvirus anguillae (AngHV-1) in European eel Anguilla anguilla (L.) originating from northern Poland - assessment of suitability of selected diagnostic methods
The Community Action Plan requests EU member states to implement measures that ensure the recovery of the severely depleted European eel stocks. One of the main threats is posed by Anguillid herpesvirus 1 (AngHV-1) leading to increased mortality in both wild and farmed eels. Following recommendation...
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ftopenagrar:oai:www.openagrar.de:openagrar_mods_00028758 2024-09-15T17:39:35+00:00 Detection of Herpesvirus anguillae (AngHV-1) in European eel Anguilla anguilla (L.) originating from northern Poland - assessment of suitability of selected diagnostic methods Nguyen, T. T. Jin, Yeonhwa Kiełpińska, J. Bergmann, Sven Lenk, Matthias Panicz, R. 2017-08-24 https://doi.org/10.1111/jfd.12689 https://www.openagrar.de/receive/openagrar_mods_00028758 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00005427/SD2017348.pdf http://onlinelibrary.wiley.com/doi/10.1111/jfd.12689/full eng eng Journal of fish diseases -- J Fish Dis -- 1365-2761 -- 0140-7775 -- 10.1111/(ISSN)1365-2761 -- 2020444-9 -- 432109-1 -- https://onlinelibrary.wiley.com/journal/13652761 -- http://www.bibliothek.uni-regensburg.de/ezeit/?2020444 https://doi.org/10.1111/jfd.12689 https://www.openagrar.de/receive/openagrar_mods_00028758 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00005427/SD2017348.pdf http://onlinelibrary.wiley.com/doi/10.1111/jfd.12689/full only signed in user all rights reserved info:eu-repo/semantics/restrictedAccess Text article ddc:570 AngHV-1 Anguilla anguilla nested PCR PCR virus isolation article Text doc-type:article 2017 ftopenagrar https://doi.org/10.1111/jfd.1268910.1111/(ISSN)1365-2761 2024-07-08T23:56:25Z The Community Action Plan requests EU member states to implement measures that ensure the recovery of the severely depleted European eel stocks. One of the main threats is posed by Anguillid herpesvirus 1 (AngHV-1) leading to increased mortality in both wild and farmed eels. Following recommendations of the OIE to minimize the risk of obtaining false-negative results, the main aim of the study was to optimize diagnostic methods for AngHV-1 detection using conventional PCR, nested PCR and in situ hybridization assay. While 53.3% of the individual organ samples were tested positive for AngHV-1 by PCR, the additional virus analysis via nested PCR revealed that the actual prevalence was 93.3%. In the cell cultivation passages, a cytopathic effect was hardly found in the first two rounds. In the third passage onto cell cultures, a lytic CPE was detected. The identification and confirmation of the viruses obtained from cell cultures as well as directly from the organ tissues were proceeded by PCR, nested PCR and sequencing of the PCR products. While no positive signal was detectable in the first round by PCR using samples from the third cell culture passages, the nested PCR provided weak but visible positive signals. Article in Journal/Newspaper Anguilla anguilla OpenAgrar (OA) Journal of Fish Diseases 40 11 1717 1723 |
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Open Polar |
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ftopenagrar |
language |
English |
topic |
Text article ddc:570 AngHV-1 Anguilla anguilla nested PCR PCR virus isolation |
spellingShingle |
Text article ddc:570 AngHV-1 Anguilla anguilla nested PCR PCR virus isolation Nguyen, T. T. Jin, Yeonhwa Kiełpińska, J. Bergmann, Sven Lenk, Matthias Panicz, R. Detection of Herpesvirus anguillae (AngHV-1) in European eel Anguilla anguilla (L.) originating from northern Poland - assessment of suitability of selected diagnostic methods |
topic_facet |
Text article ddc:570 AngHV-1 Anguilla anguilla nested PCR PCR virus isolation |
description |
The Community Action Plan requests EU member states to implement measures that ensure the recovery of the severely depleted European eel stocks. One of the main threats is posed by Anguillid herpesvirus 1 (AngHV-1) leading to increased mortality in both wild and farmed eels. Following recommendations of the OIE to minimize the risk of obtaining false-negative results, the main aim of the study was to optimize diagnostic methods for AngHV-1 detection using conventional PCR, nested PCR and in situ hybridization assay. While 53.3% of the individual organ samples were tested positive for AngHV-1 by PCR, the additional virus analysis via nested PCR revealed that the actual prevalence was 93.3%. In the cell cultivation passages, a cytopathic effect was hardly found in the first two rounds. In the third passage onto cell cultures, a lytic CPE was detected. The identification and confirmation of the viruses obtained from cell cultures as well as directly from the organ tissues were proceeded by PCR, nested PCR and sequencing of the PCR products. While no positive signal was detectable in the first round by PCR using samples from the third cell culture passages, the nested PCR provided weak but visible positive signals. |
format |
Article in Journal/Newspaper |
author |
Nguyen, T. T. Jin, Yeonhwa Kiełpińska, J. Bergmann, Sven Lenk, Matthias Panicz, R. |
author_facet |
Nguyen, T. T. Jin, Yeonhwa Kiełpińska, J. Bergmann, Sven Lenk, Matthias Panicz, R. |
author_sort |
Nguyen, T. T. |
title |
Detection of Herpesvirus anguillae (AngHV-1) in European eel Anguilla anguilla (L.) originating from northern Poland - assessment of suitability of selected diagnostic methods |
title_short |
Detection of Herpesvirus anguillae (AngHV-1) in European eel Anguilla anguilla (L.) originating from northern Poland - assessment of suitability of selected diagnostic methods |
title_full |
Detection of Herpesvirus anguillae (AngHV-1) in European eel Anguilla anguilla (L.) originating from northern Poland - assessment of suitability of selected diagnostic methods |
title_fullStr |
Detection of Herpesvirus anguillae (AngHV-1) in European eel Anguilla anguilla (L.) originating from northern Poland - assessment of suitability of selected diagnostic methods |
title_full_unstemmed |
Detection of Herpesvirus anguillae (AngHV-1) in European eel Anguilla anguilla (L.) originating from northern Poland - assessment of suitability of selected diagnostic methods |
title_sort |
detection of herpesvirus anguillae (anghv-1) in european eel anguilla anguilla (l.) originating from northern poland - assessment of suitability of selected diagnostic methods |
publishDate |
2017 |
url |
https://doi.org/10.1111/jfd.12689 https://www.openagrar.de/receive/openagrar_mods_00028758 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00005427/SD2017348.pdf http://onlinelibrary.wiley.com/doi/10.1111/jfd.12689/full |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
Journal of fish diseases -- J Fish Dis -- 1365-2761 -- 0140-7775 -- 10.1111/(ISSN)1365-2761 -- 2020444-9 -- 432109-1 -- https://onlinelibrary.wiley.com/journal/13652761 -- http://www.bibliothek.uni-regensburg.de/ezeit/?2020444 https://doi.org/10.1111/jfd.12689 https://www.openagrar.de/receive/openagrar_mods_00028758 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00005427/SD2017348.pdf http://onlinelibrary.wiley.com/doi/10.1111/jfd.12689/full |
op_rights |
only signed in user all rights reserved info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1111/jfd.1268910.1111/(ISSN)1365-2761 |
container_title |
Journal of Fish Diseases |
container_volume |
40 |
container_issue |
11 |
container_start_page |
1717 |
op_container_end_page |
1723 |
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1810480985402245120 |