Comparative impacts of elevated XO2 and acidosis on the energy budget of gill and liver cells from Atlantic cod, Gadus morhua
Ocean acidification impacts fish and other marine species through increased seawater PCO2 levels (hypercapnia). Knowledge of the physiological mechanisms mediating effects in various tissues of fish is incomplete. Here we tested the effects of extracellular hypercapnia and acidosis on energy metabol...
Published in: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2015
|
Subjects: | |
Online Access: | https://doi.org/10.1016/j.cbpa.2015.05.009 https://www.openagrar.de/receive/openagrar_mods_00024658 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00001611/dn058134.pdf |
id |
ftopenagrar:oai:www.openagrar.de:openagrar_mods_00024658 |
---|---|
record_format |
openpolar |
spelling |
ftopenagrar:oai:www.openagrar.de:openagrar_mods_00024658 2024-09-15T17:55:28+00:00 Comparative impacts of elevated XO2 and acidosis on the energy budget of gill and liver cells from Atlantic cod, Gadus morhua Stapp, Laura S. Kreiß, Cornelia Pörtner, Hans Otto Lannig, Gisela 2015 https://doi.org/10.1016/j.cbpa.2015.05.009 https://www.openagrar.de/receive/openagrar_mods_00024658 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00001611/dn058134.pdf eng eng Comparative biochemistry and physiology / A, Molecular & integrative physiology -- Comp Biochem Physiol [A] -- Comp. Biochem. Physiol. A -- Comp. Biochem. Physiol. -- CBP -- 0300-9629 -- 1095-6433 -- 1531-4332 -- 121246-1 -- 1481599-0 -- https://www.sciencedirect.com/journal/comparative-biochemistry-and-physiology-part-a-molecular-and-integrative-physiology https://doi.org/10.1016/j.cbpa.2015.05.009 https://www.openagrar.de/receive/openagrar_mods_00024658 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00001611/dn058134.pdf only signed in user all rights reserved info:eu-repo/semantics/restrictedAccess Text article ddc:590 ocean acidification -- hypnercapnia -- protein biosythesis -- free amino acids -- metabolic deprssion article Text doc-type:article 2015 ftopenagrar https://doi.org/10.1016/j.cbpa.2015.05.009 2024-07-08T23:56:25Z Ocean acidification impacts fish and other marine species through increased seawater PCO2 levels (hypercapnia). Knowledge of the physiological mechanisms mediating effects in various tissues of fish is incomplete. Here we tested the effects of extracellular hypercapnia and acidosis on energy metabolism of gill and liver cells of Atlantic cod. Exposure media mimicked blood conditions in vivo, either during normo- or hypercapnia and at control or acidic extracellular pH (pH(e)). We determined metabolic rate and energy expenditure for protein biosynthesis, Na++ATPase and H+-ATPase and considered nutrition status by measurements of metabolic rate and protein biosynthesis in media with and without free amino acids (FAA). Addition of FAA stimulated hepatic but not branchial oxygen consumption. Normo- and hypercapnic acidosis as well as hypercapnia at control pH(e) depressed metabolic stimulation of hepatocytes. In gill cells, acidosis depressed respiration independent of PCO2 and FAA levels. For both cell types, depressed respiration was not correlated with the same reduction in energy allocated to protein biosynthesis or Na+/K+-ATPase. Hepatic energy expenditure for protein synthesis and Na+/K+-ATPase was even elevated at acidic compared to control pH(e) suggesting increased costs for ion regulation and cellular reorganization. Hypercapnia at control pH(e) strongly reduced oxygen demand of branchial Na+/K+-ATPase with a similar trend for H+-ATPase. We conclude that extracellular acidosis triggers metabolic depression in gill and metabolically stimulated liver cells. Additionally, hypercapnia itself seems to limit capacities for metabolic usage of amino acids in liver cells while it decreases the use and costs of ion regulatory ATPases in gill cells. Article in Journal/Newspaper atlantic cod Gadus morhua Ocean acidification OpenAgrar (OA) Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 187 160 167 |
institution |
Open Polar |
collection |
OpenAgrar (OA) |
op_collection_id |
ftopenagrar |
language |
English |
topic |
Text article ddc:590 ocean acidification -- hypnercapnia -- protein biosythesis -- free amino acids -- metabolic deprssion |
spellingShingle |
Text article ddc:590 ocean acidification -- hypnercapnia -- protein biosythesis -- free amino acids -- metabolic deprssion Stapp, Laura S. Kreiß, Cornelia Pörtner, Hans Otto Lannig, Gisela Comparative impacts of elevated XO2 and acidosis on the energy budget of gill and liver cells from Atlantic cod, Gadus morhua |
topic_facet |
Text article ddc:590 ocean acidification -- hypnercapnia -- protein biosythesis -- free amino acids -- metabolic deprssion |
description |
Ocean acidification impacts fish and other marine species through increased seawater PCO2 levels (hypercapnia). Knowledge of the physiological mechanisms mediating effects in various tissues of fish is incomplete. Here we tested the effects of extracellular hypercapnia and acidosis on energy metabolism of gill and liver cells of Atlantic cod. Exposure media mimicked blood conditions in vivo, either during normo- or hypercapnia and at control or acidic extracellular pH (pH(e)). We determined metabolic rate and energy expenditure for protein biosynthesis, Na++ATPase and H+-ATPase and considered nutrition status by measurements of metabolic rate and protein biosynthesis in media with and without free amino acids (FAA). Addition of FAA stimulated hepatic but not branchial oxygen consumption. Normo- and hypercapnic acidosis as well as hypercapnia at control pH(e) depressed metabolic stimulation of hepatocytes. In gill cells, acidosis depressed respiration independent of PCO2 and FAA levels. For both cell types, depressed respiration was not correlated with the same reduction in energy allocated to protein biosynthesis or Na+/K+-ATPase. Hepatic energy expenditure for protein synthesis and Na+/K+-ATPase was even elevated at acidic compared to control pH(e) suggesting increased costs for ion regulation and cellular reorganization. Hypercapnia at control pH(e) strongly reduced oxygen demand of branchial Na+/K+-ATPase with a similar trend for H+-ATPase. We conclude that extracellular acidosis triggers metabolic depression in gill and metabolically stimulated liver cells. Additionally, hypercapnia itself seems to limit capacities for metabolic usage of amino acids in liver cells while it decreases the use and costs of ion regulatory ATPases in gill cells. |
format |
Article in Journal/Newspaper |
author |
Stapp, Laura S. Kreiß, Cornelia Pörtner, Hans Otto Lannig, Gisela |
author_facet |
Stapp, Laura S. Kreiß, Cornelia Pörtner, Hans Otto Lannig, Gisela |
author_sort |
Stapp, Laura S. |
title |
Comparative impacts of elevated XO2 and acidosis on the energy budget of gill and liver cells from Atlantic cod, Gadus morhua |
title_short |
Comparative impacts of elevated XO2 and acidosis on the energy budget of gill and liver cells from Atlantic cod, Gadus morhua |
title_full |
Comparative impacts of elevated XO2 and acidosis on the energy budget of gill and liver cells from Atlantic cod, Gadus morhua |
title_fullStr |
Comparative impacts of elevated XO2 and acidosis on the energy budget of gill and liver cells from Atlantic cod, Gadus morhua |
title_full_unstemmed |
Comparative impacts of elevated XO2 and acidosis on the energy budget of gill and liver cells from Atlantic cod, Gadus morhua |
title_sort |
comparative impacts of elevated xo2 and acidosis on the energy budget of gill and liver cells from atlantic cod, gadus morhua |
publishDate |
2015 |
url |
https://doi.org/10.1016/j.cbpa.2015.05.009 https://www.openagrar.de/receive/openagrar_mods_00024658 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00001611/dn058134.pdf |
genre |
atlantic cod Gadus morhua Ocean acidification |
genre_facet |
atlantic cod Gadus morhua Ocean acidification |
op_relation |
Comparative biochemistry and physiology / A, Molecular & integrative physiology -- Comp Biochem Physiol [A] -- Comp. Biochem. Physiol. A -- Comp. Biochem. Physiol. -- CBP -- 0300-9629 -- 1095-6433 -- 1531-4332 -- 121246-1 -- 1481599-0 -- https://www.sciencedirect.com/journal/comparative-biochemistry-and-physiology-part-a-molecular-and-integrative-physiology https://doi.org/10.1016/j.cbpa.2015.05.009 https://www.openagrar.de/receive/openagrar_mods_00024658 https://www.openagrar.de/servlets/MCRFileNodeServlet/openagrar_derivate_00001611/dn058134.pdf |
op_rights |
only signed in user all rights reserved info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.cbpa.2015.05.009 |
container_title |
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
container_volume |
187 |
container_start_page |
160 |
op_container_end_page |
167 |
_version_ |
1810431752938717184 |