Toll-like receptors in maraena whitefish: Evolutionary relationship among salmonid fishes and patterns of response to Aeromonas salmonicida
Toll-like receptors (TLRs) interact directly with particular pathogenic structures and are thus highly important to innate immunity. The present manuscript characterises a suite of 14 TLRs in maraena whitefish (Coregonus maraena), a salmonid species with increasing importance for aquaculture. Whitef...
Published in: | Fish & Shellfish Immunology |
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ftopenagrar:oai:www.openagrar.de:openagrar_mods_00020089 2024-09-15T17:56:30+00:00 Toll-like receptors in maraena whitefish: Evolutionary relationship among salmonid fishes and patterns of response to Aeromonas salmonicida Altmann, S. Korytar, Tomas Kaczmarzyk, D. Nipkow, M. Kühn, C. Goldammer, T. Rebl, A. 2016-04-27 https://doi.org/10.1016/j.fsi.2016.04.125 https://www.openagrar.de/receive/openagrar_mods_00020089 https://www.openagrar.de/servlets/MCRFileNodeServlet/Document_derivate_00014462/SD2016191.pdf http://www.sciencedirect.com/science/article/pii/S1050464816302765 eng eng Fish & shellfish immunology -- Fish and Shellfish Immunology -- Fish Shellfish Immunol -- 1095-9947 -- 1050-4648 -- 1467514-6 -- 1067738-0 -- http://www.sciencedirect.com/science/journal/10504648 -- http://www.bibliothek.uni-regensburg.de/ezeit/?1467514 https://doi.org/10.1016/j.fsi.2016.04.125 https://www.openagrar.de/receive/openagrar_mods_00020089 https://www.openagrar.de/servlets/MCRFileNodeServlet/Document_derivate_00014462/SD2016191.pdf http://www.sciencedirect.com/science/article/pii/S1050464816302765 only signed in user info:eu-repo/semantics/restrictedAccess article Text ddc:570 Aeromonas salmonicida Large granular lymphocytes Maraena whitefish Pathogen-associated molecular patterns Salmonidae Teleost article Text doc-type:article 2016 ftopenagrar https://doi.org/10.1016/j.fsi.2016.04.125 2024-07-08T23:56:25Z Toll-like receptors (TLRs) interact directly with particular pathogenic structures and are thus highly important to innate immunity. The present manuscript characterises a suite of 14 TLRs in maraena whitefish (Coregonus maraena), a salmonid species with increasing importance for aquaculture. Whitefish TLRs were structurally and evolutionary analysed. The results revealed a close relationship with TLRs from salmonid fish species rainbow trout and Atlantic salmon. Profiling the baseline expression of TLR genes in whitefish indicated that mainly members of the TLR11 family were highly expressed across all investigated tissues. A stimulation model with inactivated Aeromonas salmonicida was used to induce inflammation in the peritoneal cavity of whitefish. This bacterial challenge induced the expression of pro-inflammatory cytokine genes and evoked a strong influx of granulated cells of myeloid origin into the peritoneal cavity. As a likely consequence, the abundance of TLR-encoding transcripts increased moderately in peritoneal cells, with the highest levels of transcripts encoding non-mammalian TLR22a and a soluble TLR5 variant. In the course of inflammation, the proportion of granulated cells increased in peripheral blood accompanied by elevated TLR copy numbers in spleen and simultaneously reduced TLR copy numbers in head kidney at day 3 post-stimulation. Altogether, the present study provides in-vivo evidence for relatively modest TLR response patterns, but marked trafficking of myeloid cells as an immunophysiological consequence of A. salmonicida inflammation in whitefish. The present results contribute to improved understanding of the host-pathogen interaction in salmonid fish. Article in Journal/Newspaper Atlantic salmon OpenAgrar (OA) Fish & Shellfish Immunology 54 391 401 |
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language |
English |
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article Text ddc:570 Aeromonas salmonicida Large granular lymphocytes Maraena whitefish Pathogen-associated molecular patterns Salmonidae Teleost |
spellingShingle |
article Text ddc:570 Aeromonas salmonicida Large granular lymphocytes Maraena whitefish Pathogen-associated molecular patterns Salmonidae Teleost Altmann, S. Korytar, Tomas Kaczmarzyk, D. Nipkow, M. Kühn, C. Goldammer, T. Rebl, A. Toll-like receptors in maraena whitefish: Evolutionary relationship among salmonid fishes and patterns of response to Aeromonas salmonicida |
topic_facet |
article Text ddc:570 Aeromonas salmonicida Large granular lymphocytes Maraena whitefish Pathogen-associated molecular patterns Salmonidae Teleost |
description |
Toll-like receptors (TLRs) interact directly with particular pathogenic structures and are thus highly important to innate immunity. The present manuscript characterises a suite of 14 TLRs in maraena whitefish (Coregonus maraena), a salmonid species with increasing importance for aquaculture. Whitefish TLRs were structurally and evolutionary analysed. The results revealed a close relationship with TLRs from salmonid fish species rainbow trout and Atlantic salmon. Profiling the baseline expression of TLR genes in whitefish indicated that mainly members of the TLR11 family were highly expressed across all investigated tissues. A stimulation model with inactivated Aeromonas salmonicida was used to induce inflammation in the peritoneal cavity of whitefish. This bacterial challenge induced the expression of pro-inflammatory cytokine genes and evoked a strong influx of granulated cells of myeloid origin into the peritoneal cavity. As a likely consequence, the abundance of TLR-encoding transcripts increased moderately in peritoneal cells, with the highest levels of transcripts encoding non-mammalian TLR22a and a soluble TLR5 variant. In the course of inflammation, the proportion of granulated cells increased in peripheral blood accompanied by elevated TLR copy numbers in spleen and simultaneously reduced TLR copy numbers in head kidney at day 3 post-stimulation. Altogether, the present study provides in-vivo evidence for relatively modest TLR response patterns, but marked trafficking of myeloid cells as an immunophysiological consequence of A. salmonicida inflammation in whitefish. The present results contribute to improved understanding of the host-pathogen interaction in salmonid fish. |
format |
Article in Journal/Newspaper |
author |
Altmann, S. Korytar, Tomas Kaczmarzyk, D. Nipkow, M. Kühn, C. Goldammer, T. Rebl, A. |
author_facet |
Altmann, S. Korytar, Tomas Kaczmarzyk, D. Nipkow, M. Kühn, C. Goldammer, T. Rebl, A. |
author_sort |
Altmann, S. |
title |
Toll-like receptors in maraena whitefish: Evolutionary relationship among salmonid fishes and patterns of response to Aeromonas salmonicida |
title_short |
Toll-like receptors in maraena whitefish: Evolutionary relationship among salmonid fishes and patterns of response to Aeromonas salmonicida |
title_full |
Toll-like receptors in maraena whitefish: Evolutionary relationship among salmonid fishes and patterns of response to Aeromonas salmonicida |
title_fullStr |
Toll-like receptors in maraena whitefish: Evolutionary relationship among salmonid fishes and patterns of response to Aeromonas salmonicida |
title_full_unstemmed |
Toll-like receptors in maraena whitefish: Evolutionary relationship among salmonid fishes and patterns of response to Aeromonas salmonicida |
title_sort |
toll-like receptors in maraena whitefish: evolutionary relationship among salmonid fishes and patterns of response to aeromonas salmonicida |
publishDate |
2016 |
url |
https://doi.org/10.1016/j.fsi.2016.04.125 https://www.openagrar.de/receive/openagrar_mods_00020089 https://www.openagrar.de/servlets/MCRFileNodeServlet/Document_derivate_00014462/SD2016191.pdf http://www.sciencedirect.com/science/article/pii/S1050464816302765 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
Fish & shellfish immunology -- Fish and Shellfish Immunology -- Fish Shellfish Immunol -- 1095-9947 -- 1050-4648 -- 1467514-6 -- 1067738-0 -- http://www.sciencedirect.com/science/journal/10504648 -- http://www.bibliothek.uni-regensburg.de/ezeit/?1467514 https://doi.org/10.1016/j.fsi.2016.04.125 https://www.openagrar.de/receive/openagrar_mods_00020089 https://www.openagrar.de/servlets/MCRFileNodeServlet/Document_derivate_00014462/SD2016191.pdf http://www.sciencedirect.com/science/article/pii/S1050464816302765 |
op_rights |
only signed in user info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.fsi.2016.04.125 |
container_title |
Fish & Shellfish Immunology |
container_volume |
54 |
container_start_page |
391 |
op_container_end_page |
401 |
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1810432710838059008 |