Out of the reservoir: Phenotypic and genotypic characterization of a novel cowpox virus isolated from a common vole
The incidence of human cowpox virus (CPXV) infections has increased significantly in recent years. Serological surveys have suggested wild rodents as the main CPXV-reservoir. We characterized a CPXV isolated during a large scale screening from a feral common vole. A comparison of the full-length DNA...
Published in: | Journal of Virology |
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Online Access: | https://doi.org/10.1128/JVI.01195-15 https://www.openagrar.de/receive/openagrar_mods_00016548 https://www.openagrar.de/servlets/MCRFileNodeServlet/Document_derivate_00012669/SD2015299.pdf http://jvi.asm.org/content/89/21/10959 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621121/ |
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ftopenagrar:oai:www.openagrar.de:openagrar_mods_00016548 2024-09-15T18:02:47+00:00 Out of the reservoir: Phenotypic and genotypic characterization of a novel cowpox virus isolated from a common vole Hoffmann, Donata Franke, A. Jenckel, Maria Tamošiūnaitė, A. Schluckebier, Julia Granzow, Harald Hoffmann, Bernd Fischer, Stefan Ulrich, Rainer Höper, Dirk Goller, Katja Osterrieder, N. Beer, Martin 2015-08-26 https://doi.org/10.1128/JVI.01195-15 https://www.openagrar.de/receive/openagrar_mods_00016548 https://www.openagrar.de/servlets/MCRFileNodeServlet/Document_derivate_00012669/SD2015299.pdf http://jvi.asm.org/content/89/21/10959 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621121/ eng eng Journal of Virology -- J. Virol.; JVI -- 1098-5514 -- 0022-538X -- 1495529-5 -- 80174-4 -- http://jvi.asm.org/ -- https://www.ncbi.nlm.nih.gov/pmc/journals/89/ -- http://www.bibliothek.uni-regensburg.de/ezeit/?1495529 https://doi.org/10.1128/JVI.01195-15 https://www.openagrar.de/receive/openagrar_mods_00016548 https://www.openagrar.de/servlets/MCRFileNodeServlet/Document_derivate_00012669/SD2015299.pdf http://jvi.asm.org/content/89/21/10959 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621121/ public info:eu-repo/semantics/openAccess article Text ddc:570 article Text doc-type:article 2015 ftopenagrar https://doi.org/10.1128/JVI.01195-15 2024-07-08T23:56:25Z The incidence of human cowpox virus (CPXV) infections has increased significantly in recent years. Serological surveys have suggested wild rodents as the main CPXV-reservoir. We characterized a CPXV isolated during a large scale screening from a feral common vole. A comparison of the full-length DNA-sequence of this CPXV-strain with a highly virulent pet rat CPXV-isolate showed a sequence identity of 96%, including a large additional ORF of about 6,000 nucleotides, which is absent in the reference CPXV-strain Brighton Red. Electron microscopy analysis demonstrated that the vole isolate, in contrast to the rat strain, forms A-type inclusion bodies (ATI) with incorporated virions, consistent with the presence of complete ati and p4c genes. Experimental infections showed that the vole CPXV-strain caused only mild clinical symptoms in its natural host, while all rats developed severe respiratory symptoms followed by a systemic rash. In contrast, common voles infected with a high dose of the rat CPXV showed severe signs of respiratory disease but no skin lesions, whereas infection with a low dose led to virus excretion with only mild clinical signs. We concluded that the common vole is susceptible to infection with different CPXV-strains. The spectrum ranges from well-adapted viruses causing limited clinical symptoms to highly virulent strains causing severe respiratory symptoms. In addition, the low pathogenicity of the vole isolate in its eponymous host suggests a role of common voles as a major CPXV reservoir, and future research will focus on the correlation between viral genotype and phenotype/pathotype in accidental and reservoir species. Article in Journal/Newspaper Common vole OpenAgrar (OA) Journal of Virology 89 21 10959 10969 |
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article Text ddc:570 |
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article Text ddc:570 Hoffmann, Donata Franke, A. Jenckel, Maria Tamošiūnaitė, A. Schluckebier, Julia Granzow, Harald Hoffmann, Bernd Fischer, Stefan Ulrich, Rainer Höper, Dirk Goller, Katja Osterrieder, N. Beer, Martin Out of the reservoir: Phenotypic and genotypic characterization of a novel cowpox virus isolated from a common vole |
topic_facet |
article Text ddc:570 |
description |
The incidence of human cowpox virus (CPXV) infections has increased significantly in recent years. Serological surveys have suggested wild rodents as the main CPXV-reservoir. We characterized a CPXV isolated during a large scale screening from a feral common vole. A comparison of the full-length DNA-sequence of this CPXV-strain with a highly virulent pet rat CPXV-isolate showed a sequence identity of 96%, including a large additional ORF of about 6,000 nucleotides, which is absent in the reference CPXV-strain Brighton Red. Electron microscopy analysis demonstrated that the vole isolate, in contrast to the rat strain, forms A-type inclusion bodies (ATI) with incorporated virions, consistent with the presence of complete ati and p4c genes. Experimental infections showed that the vole CPXV-strain caused only mild clinical symptoms in its natural host, while all rats developed severe respiratory symptoms followed by a systemic rash. In contrast, common voles infected with a high dose of the rat CPXV showed severe signs of respiratory disease but no skin lesions, whereas infection with a low dose led to virus excretion with only mild clinical signs. We concluded that the common vole is susceptible to infection with different CPXV-strains. The spectrum ranges from well-adapted viruses causing limited clinical symptoms to highly virulent strains causing severe respiratory symptoms. In addition, the low pathogenicity of the vole isolate in its eponymous host suggests a role of common voles as a major CPXV reservoir, and future research will focus on the correlation between viral genotype and phenotype/pathotype in accidental and reservoir species. |
format |
Article in Journal/Newspaper |
author |
Hoffmann, Donata Franke, A. Jenckel, Maria Tamošiūnaitė, A. Schluckebier, Julia Granzow, Harald Hoffmann, Bernd Fischer, Stefan Ulrich, Rainer Höper, Dirk Goller, Katja Osterrieder, N. Beer, Martin |
author_facet |
Hoffmann, Donata Franke, A. Jenckel, Maria Tamošiūnaitė, A. Schluckebier, Julia Granzow, Harald Hoffmann, Bernd Fischer, Stefan Ulrich, Rainer Höper, Dirk Goller, Katja Osterrieder, N. Beer, Martin |
author_sort |
Hoffmann, Donata |
title |
Out of the reservoir: Phenotypic and genotypic characterization of a novel cowpox virus isolated from a common vole |
title_short |
Out of the reservoir: Phenotypic and genotypic characterization of a novel cowpox virus isolated from a common vole |
title_full |
Out of the reservoir: Phenotypic and genotypic characterization of a novel cowpox virus isolated from a common vole |
title_fullStr |
Out of the reservoir: Phenotypic and genotypic characterization of a novel cowpox virus isolated from a common vole |
title_full_unstemmed |
Out of the reservoir: Phenotypic and genotypic characterization of a novel cowpox virus isolated from a common vole |
title_sort |
out of the reservoir: phenotypic and genotypic characterization of a novel cowpox virus isolated from a common vole |
publishDate |
2015 |
url |
https://doi.org/10.1128/JVI.01195-15 https://www.openagrar.de/receive/openagrar_mods_00016548 https://www.openagrar.de/servlets/MCRFileNodeServlet/Document_derivate_00012669/SD2015299.pdf http://jvi.asm.org/content/89/21/10959 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621121/ |
genre |
Common vole |
genre_facet |
Common vole |
op_relation |
Journal of Virology -- J. Virol.; JVI -- 1098-5514 -- 0022-538X -- 1495529-5 -- 80174-4 -- http://jvi.asm.org/ -- https://www.ncbi.nlm.nih.gov/pmc/journals/89/ -- http://www.bibliothek.uni-regensburg.de/ezeit/?1495529 https://doi.org/10.1128/JVI.01195-15 https://www.openagrar.de/receive/openagrar_mods_00016548 https://www.openagrar.de/servlets/MCRFileNodeServlet/Document_derivate_00012669/SD2015299.pdf http://jvi.asm.org/content/89/21/10959 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621121/ |
op_rights |
public info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1128/JVI.01195-15 |
container_title |
Journal of Virology |
container_volume |
89 |
container_issue |
21 |
container_start_page |
10959 |
op_container_end_page |
10969 |
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1810440204669943808 |