Experimental infection of chicken embryos with recently described Brucella microti: Pathogenicity and pathological findings
Brucellae are facultative intracellular pathogens causing disease in a wide range of domestic and wild animals as well as in humans. Brucella microti is a recently recognized species and was isolated from common voles (Microtus arvalis), red foxes and soil in Austria and the Czech Republic. Its path...
Published in: | Comparative Immunology, Microbiology and Infectious Diseases |
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ftopenagrar:oai:www.openagrar.de:openagrar_mods_00015236 2024-09-15T18:18:46+00:00 Experimental infection of chicken embryos with recently described Brucella microti: Pathogenicity and pathological findings Wareth, Gamal Böttcher, D. Melzer, Falk Shehata, A.A. Roesler, U. Neubauer, Heinrich Karl Johann Schoon, H.-A. 2015-06-30 https://doi.org/10.1016/j.cimid.2015.06.002 https://www.openagrar.de/receive/openagrar_mods_00015236 https://www.openagrar.de/servlets/MCRFileNodeServlet/Document_derivate_00011763/SD2015226.pdf http://www.sciencedirect.com/science/article/pii/S0147957115000417 eng eng Comparative immunology, microbiology and infectious diseases -- Comp Immunol Microbiol Infect Dis -- 1878-1667 -- 0147-9571 -- 2025439-8 -- 436522-7 https://doi.org/10.1016/j.cimid.2015.06.002 https://www.openagrar.de/receive/openagrar_mods_00015236 https://www.openagrar.de/servlets/MCRFileNodeServlet/Document_derivate_00011763/SD2015226.pdf http://www.sciencedirect.com/science/article/pii/S0147957115000417 only signed in user info:eu-repo/semantics/restrictedAccess article Text ddc:570 Brucella microti Chicken embryo Experimental inoculation Histopathology Model of infection Pathogenesis article Text doc-type:article 2015 ftopenagrar https://doi.org/10.1016/j.cimid.2015.06.002 2024-07-08T23:56:25Z Brucellae are facultative intracellular pathogens causing disease in a wide range of domestic and wild animals as well as in humans. Brucella microti is a recently recognized species and was isolated from common voles (Microtus arvalis), red foxes and soil in Austria and the Czech Republic. Its pathogenicity for livestock and its zoonotic potential has not been confirmed yet. In the present study 25 SPF chicken embryos were inoculated at day 11 of age with 1.6 × 103 and 1.6 × 105Brucella microti by yolk sac and allantoic sac routes. Re-isolation of Brucella microti indicated rapid multiplication of bacteria (up to 1.7 × 1012 CFU). Brucella microti provoked marked gross lesions, i.e. hemorrhages and necroses. All inoculated embryos were dead (100% mortality) in between 2nd and 4th day post inoculation. The predominant histopathological lesion was necroses in liver, kidneys, lungs, spleen, gastrointestinal tract, spinal meninges, yolk sac and chorioallantoic membrane. Immunohistochemical examination showed the presence of Brucella antigen in nearly all of these organs, with infection being mainly restricted to non-epithelial cells or tissues. This study provides the first results on the multiplication and pathogenicity of the mouse pathogenic Brucella microti in chicken embryos. These data suggest that, even though chicken are not mammals, they could provide a useful tool for understanding the pathogenesis of Brucella microti. Article in Journal/Newspaper Microtus arvalis OpenAgrar (OA) Comparative Immunology, Microbiology and Infectious Diseases 41 28 34 |
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ftopenagrar |
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English |
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article Text ddc:570 Brucella microti Chicken embryo Experimental inoculation Histopathology Model of infection Pathogenesis |
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article Text ddc:570 Brucella microti Chicken embryo Experimental inoculation Histopathology Model of infection Pathogenesis Wareth, Gamal Böttcher, D. Melzer, Falk Shehata, A.A. Roesler, U. Neubauer, Heinrich Karl Johann Schoon, H.-A. Experimental infection of chicken embryos with recently described Brucella microti: Pathogenicity and pathological findings |
topic_facet |
article Text ddc:570 Brucella microti Chicken embryo Experimental inoculation Histopathology Model of infection Pathogenesis |
description |
Brucellae are facultative intracellular pathogens causing disease in a wide range of domestic and wild animals as well as in humans. Brucella microti is a recently recognized species and was isolated from common voles (Microtus arvalis), red foxes and soil in Austria and the Czech Republic. Its pathogenicity for livestock and its zoonotic potential has not been confirmed yet. In the present study 25 SPF chicken embryos were inoculated at day 11 of age with 1.6 × 103 and 1.6 × 105Brucella microti by yolk sac and allantoic sac routes. Re-isolation of Brucella microti indicated rapid multiplication of bacteria (up to 1.7 × 1012 CFU). Brucella microti provoked marked gross lesions, i.e. hemorrhages and necroses. All inoculated embryos were dead (100% mortality) in between 2nd and 4th day post inoculation. The predominant histopathological lesion was necroses in liver, kidneys, lungs, spleen, gastrointestinal tract, spinal meninges, yolk sac and chorioallantoic membrane. Immunohistochemical examination showed the presence of Brucella antigen in nearly all of these organs, with infection being mainly restricted to non-epithelial cells or tissues. This study provides the first results on the multiplication and pathogenicity of the mouse pathogenic Brucella microti in chicken embryos. These data suggest that, even though chicken are not mammals, they could provide a useful tool for understanding the pathogenesis of Brucella microti. |
format |
Article in Journal/Newspaper |
author |
Wareth, Gamal Böttcher, D. Melzer, Falk Shehata, A.A. Roesler, U. Neubauer, Heinrich Karl Johann Schoon, H.-A. |
author_facet |
Wareth, Gamal Böttcher, D. Melzer, Falk Shehata, A.A. Roesler, U. Neubauer, Heinrich Karl Johann Schoon, H.-A. |
author_sort |
Wareth, Gamal |
title |
Experimental infection of chicken embryos with recently described Brucella microti: Pathogenicity and pathological findings |
title_short |
Experimental infection of chicken embryos with recently described Brucella microti: Pathogenicity and pathological findings |
title_full |
Experimental infection of chicken embryos with recently described Brucella microti: Pathogenicity and pathological findings |
title_fullStr |
Experimental infection of chicken embryos with recently described Brucella microti: Pathogenicity and pathological findings |
title_full_unstemmed |
Experimental infection of chicken embryos with recently described Brucella microti: Pathogenicity and pathological findings |
title_sort |
experimental infection of chicken embryos with recently described brucella microti: pathogenicity and pathological findings |
publishDate |
2015 |
url |
https://doi.org/10.1016/j.cimid.2015.06.002 https://www.openagrar.de/receive/openagrar_mods_00015236 https://www.openagrar.de/servlets/MCRFileNodeServlet/Document_derivate_00011763/SD2015226.pdf http://www.sciencedirect.com/science/article/pii/S0147957115000417 |
genre |
Microtus arvalis |
genre_facet |
Microtus arvalis |
op_relation |
Comparative immunology, microbiology and infectious diseases -- Comp Immunol Microbiol Infect Dis -- 1878-1667 -- 0147-9571 -- 2025439-8 -- 436522-7 https://doi.org/10.1016/j.cimid.2015.06.002 https://www.openagrar.de/receive/openagrar_mods_00015236 https://www.openagrar.de/servlets/MCRFileNodeServlet/Document_derivate_00011763/SD2015226.pdf http://www.sciencedirect.com/science/article/pii/S0147957115000417 |
op_rights |
only signed in user info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.cimid.2015.06.002 |
container_title |
Comparative Immunology, Microbiology and Infectious Diseases |
container_volume |
41 |
container_start_page |
28 |
op_container_end_page |
34 |
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1810456851156828160 |