The C3 subtypes are differentially regulated after immunostimulation in rainbow trout, but head kidney macrophages do not contribute to C3 transcription
The complement system plays key roles in innate and adaptive immunity through mediating phagocytosis, chemotaxis and cell lysis. Complement component C3 is a central component in the complement cascade and belongs to the acute-phase proteins whose synthesis increase immediately upon inflammatory sti...
Published in: | Veterinary Immunology and Immunopathology |
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2007
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ftopenagrar:oai:www.openagrar.de:openagrar_mods_00012076 2023-05-15T15:31:55+02:00 The C3 subtypes are differentially regulated after immunostimulation in rainbow trout, but head kidney macrophages do not contribute to C3 transcription Lovoll, M. Fischer, Uwe Mathisen, G.S. Bogwald, J. Ototake, M Dalmo, R.A. 2007 https://doi.org/10.1016/j.vetimm.2007.03.005 https://www.openagrar.de/receive/openagrar_mods_00012076 https://www.openagrar.de/servlets/MCRFileNodeServlet/Document_derivate_00008653/SD200777.pdf eng eng Veterinary immunology and immunopathology -- Vet Immunol Immunopathol -- 0165-2427 -- 1873-2534 -- 754160-0 -- 1498915-3 https://nbn-resolving.org/10.1016/j.vetimm.2007.03.005 https://www.openagrar.de/receive/openagrar_mods_00012076 https://www.openagrar.de/servlets/MCRFileNodeServlet/Document_derivate_00008653/SD200777.pdf doi:10.1016/j.vetimm.2007.03.005 only signed in user info:eu-repo/semantics/openAccess Text ddc:630 immunostimulants subtypes head kidney macrophages regulation Atlantic salmon rainbow trout article Text 2007 ftopenagrar https://doi.org/10.1016/j.vetimm.2007.03.005 2023-03-06T00:14:46Z The complement system plays key roles in innate and adaptive immunity through mediating phagocytosis, chemotaxis and cell lysis. Complement component C3 is a central component in the complement cascade and belongs to the acute-phase proteins whose synthesis increase immediately upon inflammatory stimuli. The liver is the main producer of C3 and it is a well-known fact that the mammalian monocyte-macrophage lineage is a major contributor to extrahepatic C3. Immunomodulators, such as LPS and beta-glucan, can stimulate complement, lysozyme, natural killer cells and antibody responses in fish, thus enhancing the resistance to bacterial pathogens and parasitic infections. The aim of this study was to assess the effects of LPS and beta-glucan on the expression of interleukins (IL-1 beta 1, IL-1 beta 2 and IL-6) and the modulated expression of C3 subtypes (C3-1, C3-3 and C3-4) in the rainbow trout (Oncorhynchus mykiss) using real-time RT-PCR. From in vitro studies, we demonstrated that head kidney macrophages from rainbow trout and Atlantic salmon showed no basal transcription of C3. After immunostimulation, the cells responded by increased levels of ILs, but transcription of C3 was not induced. In contrast to the in vitro findings, the rainbow trout complement C3 subtypes were differentially regulated 48 h after in vivo stimulation with LPS and beta-glucan. These results support the previous findings of absence of C3 in macrophages of the spotted wolffish (Anarhichas minor) and is the first functional study showing differential regulation of the C3 subtypes in any vertebrate. Article in Journal/Newspaper Atlantic salmon OpenAgrar (OA) Veterinary Immunology and Immunopathology 117 3-4 284 295 |
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ftopenagrar |
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English |
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Text ddc:630 immunostimulants subtypes head kidney macrophages regulation Atlantic salmon rainbow trout |
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Text ddc:630 immunostimulants subtypes head kidney macrophages regulation Atlantic salmon rainbow trout Lovoll, M. Fischer, Uwe Mathisen, G.S. Bogwald, J. Ototake, M Dalmo, R.A. The C3 subtypes are differentially regulated after immunostimulation in rainbow trout, but head kidney macrophages do not contribute to C3 transcription |
topic_facet |
Text ddc:630 immunostimulants subtypes head kidney macrophages regulation Atlantic salmon rainbow trout |
description |
The complement system plays key roles in innate and adaptive immunity through mediating phagocytosis, chemotaxis and cell lysis. Complement component C3 is a central component in the complement cascade and belongs to the acute-phase proteins whose synthesis increase immediately upon inflammatory stimuli. The liver is the main producer of C3 and it is a well-known fact that the mammalian monocyte-macrophage lineage is a major contributor to extrahepatic C3. Immunomodulators, such as LPS and beta-glucan, can stimulate complement, lysozyme, natural killer cells and antibody responses in fish, thus enhancing the resistance to bacterial pathogens and parasitic infections. The aim of this study was to assess the effects of LPS and beta-glucan on the expression of interleukins (IL-1 beta 1, IL-1 beta 2 and IL-6) and the modulated expression of C3 subtypes (C3-1, C3-3 and C3-4) in the rainbow trout (Oncorhynchus mykiss) using real-time RT-PCR. From in vitro studies, we demonstrated that head kidney macrophages from rainbow trout and Atlantic salmon showed no basal transcription of C3. After immunostimulation, the cells responded by increased levels of ILs, but transcription of C3 was not induced. In contrast to the in vitro findings, the rainbow trout complement C3 subtypes were differentially regulated 48 h after in vivo stimulation with LPS and beta-glucan. These results support the previous findings of absence of C3 in macrophages of the spotted wolffish (Anarhichas minor) and is the first functional study showing differential regulation of the C3 subtypes in any vertebrate. |
format |
Article in Journal/Newspaper |
author |
Lovoll, M. Fischer, Uwe Mathisen, G.S. Bogwald, J. Ototake, M Dalmo, R.A. |
author_facet |
Lovoll, M. Fischer, Uwe Mathisen, G.S. Bogwald, J. Ototake, M Dalmo, R.A. |
author_sort |
Lovoll, M. |
title |
The C3 subtypes are differentially regulated after immunostimulation in rainbow trout, but head kidney macrophages do not contribute to C3 transcription |
title_short |
The C3 subtypes are differentially regulated after immunostimulation in rainbow trout, but head kidney macrophages do not contribute to C3 transcription |
title_full |
The C3 subtypes are differentially regulated after immunostimulation in rainbow trout, but head kidney macrophages do not contribute to C3 transcription |
title_fullStr |
The C3 subtypes are differentially regulated after immunostimulation in rainbow trout, but head kidney macrophages do not contribute to C3 transcription |
title_full_unstemmed |
The C3 subtypes are differentially regulated after immunostimulation in rainbow trout, but head kidney macrophages do not contribute to C3 transcription |
title_sort |
c3 subtypes are differentially regulated after immunostimulation in rainbow trout, but head kidney macrophages do not contribute to c3 transcription |
publishDate |
2007 |
url |
https://doi.org/10.1016/j.vetimm.2007.03.005 https://www.openagrar.de/receive/openagrar_mods_00012076 https://www.openagrar.de/servlets/MCRFileNodeServlet/Document_derivate_00008653/SD200777.pdf |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
Veterinary immunology and immunopathology -- Vet Immunol Immunopathol -- 0165-2427 -- 1873-2534 -- 754160-0 -- 1498915-3 https://nbn-resolving.org/10.1016/j.vetimm.2007.03.005 https://www.openagrar.de/receive/openagrar_mods_00012076 https://www.openagrar.de/servlets/MCRFileNodeServlet/Document_derivate_00008653/SD200777.pdf doi:10.1016/j.vetimm.2007.03.005 |
op_rights |
only signed in user info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.vetimm.2007.03.005 |
container_title |
Veterinary Immunology and Immunopathology |
container_volume |
117 |
container_issue |
3-4 |
container_start_page |
284 |
op_container_end_page |
295 |
_version_ |
1766362412229853184 |