Pseudogymnoascus destructans: Evidence of Virulent Skin Invasion for Bats Under Natural Conditions, Europe
While Pseudogymnoascus destructans has been responsible for mass bat mortalities from white-nose syndrome (WNS) in North America, its virulence in Europe has been questioned. To shed the light on the issue of host–pathogen interaction between European bats and P. destructans, we examined seventeen b...
Published in: | Transboundary and Emerging Diseases |
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2014
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ftopenagrar:oai:www.openagrar.de:openagrar_mods_00008423 2024-09-15T17:57:38+00:00 Pseudogymnoascus destructans: Evidence of Virulent Skin Invasion for Bats Under Natural Conditions, Europe Bandouchova, H. Bartonicka, T. Berkova, H. Brichta, J. Cerny, J. Kovacova, V. Kolarik, M. Köllner, Bernd Kulich, P. Martinkova, N. Rehak, Z. Turner, G.G. Zukal, J. Pikula, J. 2014-09-30 https://doi.org/10.1111/tbed.12282 https://www.openagrar.de/receive/openagrar_mods_00008423 https://www.openagrar.de/servlets/MCRFileNodeServlet/Document_derivate_00009022/SD201525.pdf http://onlinelibrary.wiley.com/doi/10.1111/tbed.12282/abstract eng eng Transboundary and Emerging Diseases -- Transbound Emerg Dis -- 1865-1682 -- 1865-1674 -- 2414822-2 -- 2408860-2 -- 10.1111/(ISSN)1865-1682 -- https://www.hindawi.com/journals/tbed/ -- https://onlinelibrary.wiley.com/journal/18651682 -- http://www.bibliothek.uni-regensburg.de/ezeit/?2414822 https://doi.org/10.1111/tbed.12282 https://www.openagrar.de/receive/openagrar_mods_00008423 https://www.openagrar.de/servlets/MCRFileNodeServlet/Document_derivate_00009022/SD201525.pdf http://onlinelibrary.wiley.com/doi/10.1111/tbed.12282/abstract only signed in user info:eu-repo/semantics/restrictedAccess article Text ddc:630 white-nose syndrome Chiroptera transmission electron microscopy ultraviolet light diagnosis morbidity mortality article Text doc-type:article 2014 ftopenagrar https://doi.org/10.1111/tbed.1228210.1111/(ISSN)1865-1682 2024-07-08T23:56:23Z While Pseudogymnoascus destructans has been responsible for mass bat mortalities from white-nose syndrome (WNS) in North America, its virulence in Europe has been questioned. To shed the light on the issue of host–pathogen interaction between European bats and P. destructans, we examined seventeen bats emerging from the fungus-positive underground hibernacula in the Czech Republic during early spring 2013. Dual wing-membrane biopsies were taken from Barbastella barbastellus (1), Myotis daubentonii (1), Myotis emarginatus (1), Myotis myotis (11), Myotis nattereri (1) and Plecotus auritus (2) for standard histopathology and transmission electron microscopy. Non-lethal collection of suspected WNS lesions was guided by trans-illumination of the wing membranes with ultraviolet light. All bats selected for the present study were PCR-positive for P. destructans and showed microscopic findings consistent with the histopathological criteria for WNS diagnosis. Ultramicroscopy revealed oedema of the connective tissue and derangement of the fibroblasts and elastic fibres associated with skin invasion by P. destructans. Extensive fungal infection induced a marked inflammatory infiltration by neutrophils at the interface between the damaged part of the wing membrane replaced by the fungus and membrane tissue not yet invaded by the pathogen. There was no sign of keratinolytic activity in the stratum corneum. Here, we show that lesions pathognomonic for WNS are common in European bats and may also include overwhelming full-thickness fungal growth through the wing membrane equal in severity to reports from North America. Inter-continental differences in the outcome of WNS in bats in terms of morbidity/mortality may therefore not be due to differences in the pathogen itself. Article in Journal/Newspaper Barbastella barbastellus Myotis nattereri OpenAgrar (OA) Transboundary and Emerging Diseases 62 1 1 5 |
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English |
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article Text ddc:630 white-nose syndrome Chiroptera transmission electron microscopy ultraviolet light diagnosis morbidity mortality |
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article Text ddc:630 white-nose syndrome Chiroptera transmission electron microscopy ultraviolet light diagnosis morbidity mortality Bandouchova, H. Bartonicka, T. Berkova, H. Brichta, J. Cerny, J. Kovacova, V. Kolarik, M. Köllner, Bernd Kulich, P. Martinkova, N. Rehak, Z. Turner, G.G. Zukal, J. Pikula, J. Pseudogymnoascus destructans: Evidence of Virulent Skin Invasion for Bats Under Natural Conditions, Europe |
topic_facet |
article Text ddc:630 white-nose syndrome Chiroptera transmission electron microscopy ultraviolet light diagnosis morbidity mortality |
description |
While Pseudogymnoascus destructans has been responsible for mass bat mortalities from white-nose syndrome (WNS) in North America, its virulence in Europe has been questioned. To shed the light on the issue of host–pathogen interaction between European bats and P. destructans, we examined seventeen bats emerging from the fungus-positive underground hibernacula in the Czech Republic during early spring 2013. Dual wing-membrane biopsies were taken from Barbastella barbastellus (1), Myotis daubentonii (1), Myotis emarginatus (1), Myotis myotis (11), Myotis nattereri (1) and Plecotus auritus (2) for standard histopathology and transmission electron microscopy. Non-lethal collection of suspected WNS lesions was guided by trans-illumination of the wing membranes with ultraviolet light. All bats selected for the present study were PCR-positive for P. destructans and showed microscopic findings consistent with the histopathological criteria for WNS diagnosis. Ultramicroscopy revealed oedema of the connective tissue and derangement of the fibroblasts and elastic fibres associated with skin invasion by P. destructans. Extensive fungal infection induced a marked inflammatory infiltration by neutrophils at the interface between the damaged part of the wing membrane replaced by the fungus and membrane tissue not yet invaded by the pathogen. There was no sign of keratinolytic activity in the stratum corneum. Here, we show that lesions pathognomonic for WNS are common in European bats and may also include overwhelming full-thickness fungal growth through the wing membrane equal in severity to reports from North America. Inter-continental differences in the outcome of WNS in bats in terms of morbidity/mortality may therefore not be due to differences in the pathogen itself. |
format |
Article in Journal/Newspaper |
author |
Bandouchova, H. Bartonicka, T. Berkova, H. Brichta, J. Cerny, J. Kovacova, V. Kolarik, M. Köllner, Bernd Kulich, P. Martinkova, N. Rehak, Z. Turner, G.G. Zukal, J. Pikula, J. |
author_facet |
Bandouchova, H. Bartonicka, T. Berkova, H. Brichta, J. Cerny, J. Kovacova, V. Kolarik, M. Köllner, Bernd Kulich, P. Martinkova, N. Rehak, Z. Turner, G.G. Zukal, J. Pikula, J. |
author_sort |
Bandouchova, H. |
title |
Pseudogymnoascus destructans: Evidence of Virulent Skin Invasion for Bats Under Natural Conditions, Europe |
title_short |
Pseudogymnoascus destructans: Evidence of Virulent Skin Invasion for Bats Under Natural Conditions, Europe |
title_full |
Pseudogymnoascus destructans: Evidence of Virulent Skin Invasion for Bats Under Natural Conditions, Europe |
title_fullStr |
Pseudogymnoascus destructans: Evidence of Virulent Skin Invasion for Bats Under Natural Conditions, Europe |
title_full_unstemmed |
Pseudogymnoascus destructans: Evidence of Virulent Skin Invasion for Bats Under Natural Conditions, Europe |
title_sort |
pseudogymnoascus destructans: evidence of virulent skin invasion for bats under natural conditions, europe |
publishDate |
2014 |
url |
https://doi.org/10.1111/tbed.12282 https://www.openagrar.de/receive/openagrar_mods_00008423 https://www.openagrar.de/servlets/MCRFileNodeServlet/Document_derivate_00009022/SD201525.pdf http://onlinelibrary.wiley.com/doi/10.1111/tbed.12282/abstract |
genre |
Barbastella barbastellus Myotis nattereri |
genre_facet |
Barbastella barbastellus Myotis nattereri |
op_relation |
Transboundary and Emerging Diseases -- Transbound Emerg Dis -- 1865-1682 -- 1865-1674 -- 2414822-2 -- 2408860-2 -- 10.1111/(ISSN)1865-1682 -- https://www.hindawi.com/journals/tbed/ -- https://onlinelibrary.wiley.com/journal/18651682 -- http://www.bibliothek.uni-regensburg.de/ezeit/?2414822 https://doi.org/10.1111/tbed.12282 https://www.openagrar.de/receive/openagrar_mods_00008423 https://www.openagrar.de/servlets/MCRFileNodeServlet/Document_derivate_00009022/SD201525.pdf http://onlinelibrary.wiley.com/doi/10.1111/tbed.12282/abstract |
op_rights |
only signed in user info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1111/tbed.1228210.1111/(ISSN)1865-1682 |
container_title |
Transboundary and Emerging Diseases |
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62 |
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5 |
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1810433775067201536 |